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OSTN gene fragment used as molecular marker for porcine immune traits and applications of molecular marker

A technology of molecular markers and traits, applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of pig death, food safety hazards, and the difficulty of disease prevention and control in farms due to drug resistance of bacteria , to achieve good specificity and high sensitivity

Active Publication Date: 2019-04-12
HUAZHONG AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The outbreak of diseases in the process of pig breeding can directly lead to the death of pigs and reduce the slaughter rate, which in turn affects the economic benefits of pig farming
And in order to prevent diseases, a large number of drugs are used to increase the cost of breeding and drug residues, and the residual drugs destroy the ecological environment
In particular, the abuse of antibiotics not only leads to the emergence of drug resistance of bacteria and increases the difficulty of disease prevention and control in farms, but also threatens the quality and safety of pork, causing food safety hazards

Method used

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  • OSTN gene fragment used as molecular marker for porcine immune traits and applications of molecular marker
  • OSTN gene fragment used as molecular marker for porcine immune traits and applications of molecular marker
  • OSTN gene fragment used as molecular marker for porcine immune traits and applications of molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Genotyping Detection

[0037] (1) Using phenol extraction method to extract ear tissue DNA from Duroc×Erhualian F2 pig population

[0038] 1) Grind the ear tissue of Duroc × Erhualian F2 population (provided by Guangdong Huanong Wens Animal Husbandry Co., Ltd.) in liquid nitrogen, add an equal volume of 1 × SET (1mL), proteinase K (10ng / mL) To a final concentration of 200ug / mL, sodium dodecyl sulfate (ie SDS, 10%) to a final concentration of 0.5%, shake well. Incubate overnight in a 55°C water bath for digestion.

[0039] 2) Add an equal volume of Tris-saturated phenol to the digested tissue sample, slowly invert the centrifuge tube for 15 minutes, centrifuge in a low-temperature refrigerated centrifuge at 4°C and 11,000 rpm for 10 minutes, carefully transfer the supernatant to another centrifuge tube, and label with corresponding mark.

[0040] 3) Add an equal volume of phenol / chloroform / isoamyl alcohol (volume ratio 25:24:1), slowly invert the centrifuge...

Embodiment 2

[0074] Example 2: Application of OSTN molecular marker typing method in correlation analysis of pig immune traits

[0075] (1) Correlation analysis between OSTN molecular marker typing results and immune traits

[0076] The experimental pigs used for the detection and analysis of the association between genotype and immune traits come from the F2 generation population (conventional breed) of Duroc×Erhualian crossbred by Guangdong Huanong Wen's Animal Husbandry Co., Ltd. The DNA used for genotyping was extracted from the ear sample of the F2 generation of Duroc×Erhualian hybrid ("Duroc×Erhualian hybrid F2 generation" referred to as "pig" in the following text and table) was used for blood routine testing and The blood for flow cytometric analysis was collected from pigs aged 20, 33, 35 and 80 days. Pigs aged 20 and 33 were not inoculated with polymyocytes (PolyI:C), and piglets at the age of 35 were inoculated with polymyocytes (PolyI:C). :C) Blood samples were collected 4 hou...

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Abstract

The invention belongs to the technical field of porcine molecular marker screening, and particularly relates to OSTN gene fragment used as a molecular marker for porcine immune traits and applicationsof the molecular marker. The molecular marker is cloned from the porcine OSTN gene. The present invention screens out a molecular marker associated with porcine immune traits, the nucleotide sequenceof the molecular marker is as shown in SEQ ID NO:1, and an A / T allelic mutation exists at the 37th base of the sequence. The mutation results in Sequenom SNP typing polymorphism. The present invention discloses a method for screening the molecular marker and applications of an associated analytical method. The present invention provides a novel SNP molecular marker resource for marker-assisted selection of porcine immune traits.

Description

technical field [0001] The invention belongs to the technical field of pig molecular marker screening, and specifically relates to the molecular marker and application of OSTN gene fragments as pig immune traits. Background technique [0002] The pig industry occupies an important position in my country's animal husbandry. In recent years, not only have breeds or strains with local characteristics and excellent production performance been bred, but also the introduction of foreign breeds has accelerated the genetic improvement of pig quality, which has promoted the development of my country's pig industry. . With the popularization of the intensive production model, the problems of epidemic diseases have come one after another, mainly manifested in the weak ability to control certain epidemic diseases, and the improper handling of disease detection, diagnosis, prevention and extermination. Therefore, the disease problem is one of the key factors affecting the development of ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 赵书红李新云赵志超栾宁刘华珍刘向东朱猛进刘小磊马云龙余梅刘望宏施亮李家连钱平
Owner HUAZHONG AGRICULTURAL UNIVERSITY