SgRNA (small guide Ribonucleic Acid) for specifically identifying swine Tert (Telomerase Reverse Transcriptase) locus and encoding DNA (Deoxyribonucleic Acid) and application thereof

A specific recognition and locus technology, applied in the field of genetic engineering, can solve the problem that the molecular mechanism has not been fully resolved, and achieve the effect of efficient large fragment knockout and strong specificity
CN109679958AActive Publication Date: 2019-04-26FOSHAN UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
FOSHAN UNIVERSITY
Publication Date
2019-04-26

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Abstract

The invention provides sgRNA (small guide Ribonucleic Acid) for specifically identifying a swine Tert (Telomerase Reverse Transcriptase) locus. The sgRNA is formed by pTert-exon1-sgRNA and pTert-exon2-sgRNA, wherein a nucleotide sequence which is responsible for target fragments in the pTert-exon1-sgRNA is SEQ ID NO.1; a nucleotide sequence which is responsible for target fragments in the pTert-exon2-sgRNA is SEQ ID NO.2. The sgRNA for the swine Tert locus is strong in specificity. The sgRNA for the swine Tert locus can utilize CRISPR / Cas9 gene editing system and a bifluorescence enrichment method and is capable of efficiently realizing large-fragment knockout of the swine Tert gene, the homozygote efficiency can be up to 50 percent, and preliminary technical support is provided for follow-up research on swine Tert gene functions.
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Description

technical field

[0001] The invention relates to the technical field of genetic engineering, in particular to sgRNA specifically recognizing pig Tert site, its coding DNA and application. Background technique

[0002] Aging is the biggest risk factor for human diseases, and the risks of tumors, diabetes, cardiovascular diseases, and Alzheimer's all increase year by year with age. Telomere reverse transcriptase (Tert) gene plays an important role in the process of aging, but its molecular mechanism of regulating animal aging has not been fully resolved. The physiology and other aspects of pigs are highly similar to humans, making them ideal models for human diseases. If pigs are used as the research object, the Tert gene-edited pig cell model will be prepared by genome editing technology, and the molecular mechanism of Tert gene regulating pig aging phenotype will be revealed, which will provide new ideas for the mechanism elucidation of human aging-related diseases and the d...

Claims

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