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A kind of dna extraction method

An extraction method and technology of an extraction device, which are applied in the fields of biotechnology and DNA extraction, can solve the problems of cumbersome operation steps for DNA extraction by magnetic beads method, high price of an automatic nucleic acid extractor, and low extraction purity, and simplify the DNA extraction process. , The effect of reducing skill requirements and saving experimental time

Active Publication Date: 2022-05-17
苏州含光微纳科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The operation steps of DNA extraction by magnetic bead method are cumbersome and the extraction purity is lower than that of silica gel membrane adsorption method. The price of fully automatic nucleic acid extraction instrument is beyond the tolerance of hospitals in remote areas and township health centers.

Method used

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  • A kind of dna extraction method
  • A kind of dna extraction method
  • A kind of dna extraction method

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Embodiment Construction

[0036] To make the above-described objects, features and advantages of the present invention can be more obvious and understandable, the following through specific embodiments and accompanying drawings, the present invention will be further elaborated in detail.

[0037] One. DNA extraction apparatus employed in the present invention

[0038] The DNA extraction apparatus employed in the present invention comprises a centrifuge and a DNA extraction centrifuge dish, the DNA extraction centrifuge dish is mounted on the centrifuge. as Figure 1As shown, the DNA extraction centrifuge disc of the present embodiment 100 is provided with a plurality of DNA extraction units 110 evenly distributed along the circumference. The solid line part is indicated to be located on the front of the centrifuge disc, and the dashed part is indicated to be located on the back of the centrifuge disc. Figure 2 The structure of the front of the centrifuge disc is shown separately, Figure 3 The structure on...

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Abstract

The invention relates to a DNA extraction method. The method comprises: adding blood and red blood cell lysate into the first liquid inlet, adding high-concentration salt ion solution into the second liquid inlet, adding DNA eluent into the third liquid inlet; The lysate is mixed evenly; the centrifuge rotates at speed 1, so that the solutions in the first liquid inlet and the second liquid inlet enter the filter area and mix; the centrifuge rotates at speed 2, so that the DNA in the filter area adheres to the DNA adsorption In terms of materials, the remaining impurities enter the first liquid storage area, and at the same time, the eluent in the third liquid inlet enters the temporary liquid storage area; the centrifugal disc rotates at a speed of 3, so that the DNA eluent in the temporary liquid storage area enters the filter area, And the impurities in the first liquid storage area enter the second liquid storage area; the centrifuge rotates at a speed of 4, so that the eluted DNA enters the first liquid storage area; and the DNA is taken out from the first liquid storage area. The invention can efficiently realize DNA extraction.

Description

Technical field [0001] The present invention belongs to the field of biotechnology, DNA extraction technology, specifically relates to a DNA extraction method, can be conveniently and efficiently realized DNA extraction. Background [0002] The main methods of DNA extraction are as follows: [0003] 1) Classic phenol-chloroform method. Generally refers to the addition of an equal volume of phenol chloroform mixture after cell lysis, according to the characteristics of DNA is easily soluble in water, not easy to dissolve in organic solvents, in the organic solvent environment, protein after denaturation precipitation and centrifugation, organic solvents exist in the bottom of the test tube, DNA exists in the upper water, protein precipitation exists between the two liquids, and finally with ethanol to separate the DNA from the water, precipitate, centrifugation after recovery. The phenol-chloroform extraction process requires multiple complex centrifugal operations, takes a long ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/10
CPCC12N15/1006C12N15/1017
Inventor 吴烨娴安光明陈兢
Owner 苏州含光微纳科技有限公司
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