Preparation method and application of planktonic algae containing pseudovacua
一种浮游藻类、浮游藻的技术，应用在浮游藻类的制备领域，能够解决细胞死亡、效率低、成本高等问题，达到生物安全性高、提高增殖速率、易于操作的效果
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[0037] In yet another aspect, the embodiments of the present invention also provide a method for preparing the planktonic algae containing pseudovacuoles in the above embodiments. The process flow of the method for screening and expanding the cultivation of the planktonic algae containing pseudovacuum is as follows: figure 1 As shown, it includes the following steps:
[0038] Step S01: inoculating planktonic algae containing pseudophyllocysts into the primary planktonic algae culture medium, and injecting sterile oxygen-containing gas into the planktonic algae culture medium for sterile aerobic culture treatment;
[0039] Step S02: After the planktonic algae are cultivated to the logarithmic growth phase, inoculate the planktonic algae in the logarithmic growth phase into the planktonic algae expansion medium for sterile aerobic expansion culture treatment;
[0040] Step S03: statically treat the phytoplankton that has undergone the aseptic aerobic expansion culture, and then...
Embodiment 1
[0064] Embodiment 1: the cultivation method of the planktonic algae containing pseudovacuum
[0065] The present embodiment provides a method for cultivating planktonic algae containing pseudovacuoles, comprising the steps of:
[0066] S11: Cultivation of Anabaena blooms in stagnation period (about 10 days): Open the lid of Anabaena blooms freeze-dried powder (purchased from ATCC, American Biological Standards Collection Center), place in a sterile environment at room temperature for one day to rewarm, Pour the freeze-dried powder into a 50ml Erlenmeyer flask, add cyanobacteria BG11 medium at a volume ratio of 1:50, shake well, insert a ventilation catheter into the Erlenmeyer flask to connect to the air pump, and fill the mouth of the Erlenmeyer flask with sterilized cotton, Seal it with a sealing strip (paraflim) to keep the bottle in a sterile environment; the culture light time is 14h light / 10h dark, the temperature is 25°C, and the shaker speed is 100rpm. After about 6 da...
Embodiment 2
[0070] Embodiment 2: the cultivation method of the planktonic algae containing pseudovacuum
[0071] The present embodiment provides a method for cultivating planktonic algae containing pseudovacuoles, comprising the steps of:
[0072] S11: Cultivation of Microcystis stagnation period (about 10 days): Pour Microcystis into a 50ml Erlenmeyer flask, add cyanobacteria BG11 medium at a volume ratio of 1:50, shake well, and insert a ventilation tube into the Erlenmeyer flask Connect the air pump, fill the mouth of the Erlenmeyer bottle with sterilized cotton, and seal it with a sealing strip (paraflim) to keep the bottle in a sterile environment; the culture light time is 14h light / 10h dark, the temperature is 25°C, and the shaker speed is 100rpm. After about 6 days of culture, when the culture medium changes from the initial colorless and transparent to light green, add an equal amount of fresh BG11 culture medium to continue the culture, and when the green color of the culture me...
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