Preparation technology of holothurian fucoidan and enzymatic hydrolysate of holothurian fucoidan

Abstract

The invention discloses a preparation technology of holothurian fucoidan and enzymatic hydrolysate of the holothurian fucoidan. The preparation technology takes the holothurian fucoidan as a raw material and comprises the following steps of breaking glucosidic bonds by an ultrasonic-microwave joint degrading method, and decomposing the holothurian fucoidan to obtain low-molecular weight holothurian fucoidan; selecting methyl methanesulfonate, and performing sulfate esterifying reaction on the methyl methanesulfonate and the low-molecular weight holothurian fucoidan, so as to obtain the low-molecular weight and highly-sulfated holothurian fucoidan. The preparation method provided by the invention has the advantages that the reaction cost is lower, the preparation process is simple, the reaction efficiency is higher, the pollution is avoided, the destroy to the structure of the product is little, no side reaction occurs, and the reaction is mild and controllable; the prepared product haslow molecular weight, high content of sulfate radicals, stronger biological activity, has anti-oxidizing, blood glucose reducing and blood lipid reducing effects, is suitable for a human body to absorb, can be applied to the corresponding industries of health-care product, functional food or medical formula food and the like, and has extremely high social and economic meaning and application potential.

Description

technical field [0001] The invention relates to the technical field of biological extraction, in particular to a preparation process of sea cucumber fucoidan sulfate ester and an enzymatic hydrolysis product thereof. Background technique [0002] Sea cucumber is a traditional medicinal and food tonic in my country. Among the 1,100 species of sea cucumbers in the world, only 20 are edible, and most of them are inedible low-value sea cucumbers. The body wall of sea cucumber is the main edible or medicinal part of sea cucumber. In dried sea cucumber, the protein content is as high as 85-90%, the lipid accounts for about 4%, and the polysaccharide accounts for about 6-10%. In addition, it also contains calcium, iron, zinc, manganese, selenium, molybdenum and other trace elements. The body wall of sea cucumber contains a variety of physiologically active substances, such as collagen, sulfated polysaccharides, saponins, sterols, active lipids, etc. [0003] Sulfated polysacchari...

AI Technical Summary

Problems solved by technology

Foreign reports are to use cesium hydroxide and trimethylammonium sulfur trioxide copolymer to react with fucoidan sulfate under the joint action to prepare oversulfated fucoidan sulfate. The sulfation level of the product prepared by this method is Higher, but trimethylammonium sulfur trioxide copolymer is expensive, which is not conducive to industrial production