Improved polyoxin fermentation culture medium and fermentation process

A fermentation medium, polyoxin technology, applied in the direction of fermentation, microorganism-based methods, biochemical equipment and methods, etc., can solve the problem that the viscosity and the elimination of foam during sterilization cannot be scientifically controlled, and there is no special research Over-fermentation medium quality stability, medium quality instability and other issues, to achieve the effect of shortening the fermentation cycle, reducing air demand, and reducing ventilation

Pending Publication Date: 2019-05-28
陕西麦可罗生物科技有限公司
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The existing polyoxin fermentation process has the following problems: 1. Long-term maintenance of an alkaline pH value environment causes polyoxin to be unstable in an alkaline environment; 2. The viscosity of the medium and the time of sterilization Foam elimination cannot be scientifically controlled during autoclaving of existing media
[0006] But the above prior art all has not specially studied the sta

Method used

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  • Improved polyoxin fermentation culture medium and fermentation process
  • Improved polyoxin fermentation culture medium and fermentation process
  • Improved polyoxin fermentation culture medium and fermentation process

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0031] Example 1

[0032] The fermentation medium of the present invention: weigh 1.5% corn flour, 2.0% soybean meal powder, 2.0% caramel, and KH according to the formula 2 PO 4 0.1%, NaCl 0.1%, CaCO 3 0.3%, (NH 4 ) 2 SO 4 0.5%, antifoaming agent 0.035%, pH was adjusted to 6.5 before adding anticannoning agent, and sterilized at 121°C for 30 minutes to obtain the fermentation medium of the present invention.

Example Embodiment

[0033] Example 2

[0034] The fermentation medium of Example 1 adopts the fermentation process of the present invention as follows: Inoculate the slant spores of Streptomyces aureus with the model number 4896 into a seed shake flask at 28° C. and 220 rpm for 30 hours, and then bottle it into shake flask seeds. The shake flask seeds were inoculated into the first-level seed tank at 28°C for 24h, and then transplanted into the sterilized fermentation medium prepared in Example 1. At 28°C, 110rpm, tank pressure 0.05MPa, and ventilation volume 0.5-1.0L / Min continues to ferment. Feeding (NH 4 ) 2 SO 4 And ammonia water, add nitrogen source while adjusting the pH to maintain at 6.3-6.5, and put it in the tank when the culture is 113h.

Example Embodiment

[0035] Example 3

[0036] The experimental results of Comparative Examples 1 and 2 and Examples 1 and 2 are compared as follows:

[0037] figure 1 In order to use the original fermentation process of the original fermentation medium, that is, Comparative Example 2, the diagram of the hypha at 30 hours shows that it forms a small net, and the hypha is long and less. figure 2 In order to use the fermentation process of the fermentation medium of the present invention, that is, the diagram of the hyphae at 30h, it can be seen that the hyphae form medium to large meshes, with long hyphae and complex cross-linking, less hyphae, and deep staining. .

[0038] image 3 In order to use the original fermentation process of the original fermentation medium, that is, Comparative Example 2, the diagram of the hypha at 68 hours shows that it forms a large network with long hyphae and complex cross-links. Figure 4 In order to use the fermentation process of the fermentation medium of the present ...

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Abstract

The invention relates to an improved polyoxin fermentation culture medium and a fermentation process. The polyoxin fermentation culture medium is prepared from the following ingredients: 1% to 2% of maize meal, 1.5% to 3% of soybean cake meal, 1.5% to 3% of cerealose, 0.05% to 0.2% of KH2PO4, 0.05% to 0.2% of NaCl, 0.15% to 0.45% of CaCO3, 0.025% to 0.050% of antifoamer, 0.1% to 2% of (NH4)2SO4andthe balance of aqua sterilisa. According to the improved polyoxin fermentation culture medium and the fermentation process, the fermentation process is optimized, the stability of the fermentation culture medium is improved, and the biological value of the fermentation process is also increased.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to an improved polyoxin fermentation medium and fermentation process. Background technique [0002] Polyoxin (Polyoxin) has a good systemic conduction effect, is a broad-spectrum antibiotic fungicide, is a safe pesticide with high efficiency, low toxicity, and no environmental pollution, so it is widely used in food crops, fruits and vegetables and other important diseases. It is mainly used to control more than ten kinds of crop diseases such as apple leaf spot, rice sheath blight, ring spot, pear black spot, grape gray mold, downy mildew, ginseng black spot and tobacco red spot. [0003] Most of the existing polyoxin raw materials are obtained from the wild-type stock of Streptomyces aureochromogenes No4896 through microbial fermentation, but the fermentation unit is not high and unstable. The technological process of the bacteria fermenting polyoxin i...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P21/02C12R1/465
CPCY02P60/87
Inventor 杨玉旺潘忠成李昶志李蒲民
Owner 陕西麦可罗生物科技有限公司
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