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Radiation sensitive gene marker and application thereof in identifying low LET ray radiation

A genetic marker and radiation-sensitive technology, applied in the field of radiation biological radiation type detection, can solve the problem of inability to completely block X-rays

Active Publication Date: 2019-05-31
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the daily extensive use of CT examinations and tumor radiation therapy, especially in orthopedics, tumor intervention, and cardiac conduction bundle branch ablation operations based on digital radiography or digital subtraction angiography, the radiation protection tools for medical staff include lead clothing, scarves, etc. , goggles, etc. are often unable to completely block the X-ray during the operation

Method used

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  • Radiation sensitive gene marker and application thereof in identifying low LET ray radiation
  • Radiation sensitive gene marker and application thereof in identifying low LET ray radiation
  • Radiation sensitive gene marker and application thereof in identifying low LET ray radiation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1 Screening and Obtaining of Radiation Sensitive Gene Markers

[0059] 1. Animal experiment irradiation and grouping: 8-10 weeks old C57BL6 female mice, SPF rearing, and feeding conditions are: temperature (23±1)℃, relative humidity 55%±5%, pressure difference ≤10Pa, daily Light for 12h, and eat and drink freely.

[0060] 2. After induction of anesthesia by isoflurane before irradiation, transfer to a dedicated mouse chest irradiation device and fix it properly. X-ray and heavy ion (carbon-ions) rays are used to irradiate the whole lung field. X-ray is one of the representatives of low LET rays, which is irradiated after being accelerated by a medical linear accelerator (energy 6-10MV). As the main representative of high LET rays, heavy ion rays are carbon particle beams (energy 280MeV) that are accelerated by a cyclotron (20-30 meters in diameter) and then discharged, with extremely high energy and speed.

[0061] 3. Irradiation and grouping include X-ray gradient ...

Embodiment 2

[0089] This implementation provides the application of the radiation sensitive gene markers screened in Example 1 in identifying low LET radiation.

[0090] See Figure 4 , The expression multiples of 10 genes after gradient-dose X-rays and carbon ion beams were irradiated. There was almost no radiation response in the heavy ion beam irradiation group.

[0091] See Figure 5 , The difference in gene expression values ​​of 10 genes under gradient dose X-ray and carbon ion radiation for 24 weeks, the difference was statistically significant (P <0.0001).

[0092] See Image 6 , Receiver operating characteristic curve (receiver operating characteristic curve, ROC) graph, 10 genes in the gradient dose X-ray, carbon ion radiation after the average gene expression of the X-ray identification ability. The area under the curve (AUC) is equal to 0.984, the 95% confidence interval (Confidence interval, CI) is 0.952–1.000, and when the average expression of the above 10 genes is equal to 1.033,...

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Abstract

The invention provides a radiation sensitive gene marker and application thereof in identifying low LET ray radiation. The radiation sensitive gene marker includes Wnt7b, Tprkb, Pira1, Pde4dip, Limk2,Ctns, Kcnk6, Csf2rb, Cd80 and Sesn2. The gene expression level of the radiation sensitive gene marker in an irradiated organism is detected to identify whether the irradiated organism is exposed to the low LET ray radiation (for example: X-rays, beta rays, gamma rays and the like) to provide radiation dose reference for risk assessment of nuclear radiation damage.

Description

Technical field [0001] The invention belongs to the field of radiation biological radiation type detection, and specifically relates to radiation sensitive gene markers and their application in identifying low LET radiation. Background technique [0002] X-rays and other low linear energy transfer (Linear energy transfer, LET) rays such as β, γ-rays, electronic lines, etc. have a broad application basis in various fields such as medical and health, industrial and agricultural production, special medicine, and scientific research. LET is a parameter for evaluating radiographic quality. Compared with high LET rays (such as heavy ions, fast neutrons, carbon, nitrogen, oxygen, neon, etc.), low LET rays have a significantly smaller ionization density per unit length, and the scattering effect of rays is very significant. In addition to the penetration and fluorescence effects of electromagnetic waves, X-ray radiation protection has also become an issue of increasing concern. [0003] ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/11
Inventor 周平坤周兆明周成刘晓丹
Owner ACADEMY OF MILITARY MEDICAL SCI
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