Application of beckmannia syzigachne CYP704B1 gene

A technology of CYP704B1 and sagegrass, applied in the field of genetic engineering, can solve problems such as the difficulty of sagegrass management
CN109837355AActive Publication Date: 2019-06-04INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
3 Cites 1 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
Publication Date
2019-06-04

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention provides an application of a beckmannia syzigachne CYP704B1 gene. The invention further provides a fluorescent quantitation PCR primer (SEQ ID NO:2-3) for detecting the expression quantity of the beckmannia syzigachne CYP704B1 gene and a method for detecting the expression quantity of the beckmannia syzigachne CYP704B1 gene established based on the primer. The method is used for judging the resistance of beckmannia syzigachne to sulfonylurea type (especially mesosulfuron) herbicides. The method has the advantages of being simple, convenient, high in sensitivity and high in specificity, and has important application prospects in detection and research of the non-target resistance of the beckmannia syzigachne on the mesosulfuron. According to the application disclosed by the invention, the relationship of the beckmannia syzigachne CYP704B1 gene and the resistance of the sulfonylurea type herbicides is revealed for the first time, whether the beckmannia syzigachne generatesmetabolic resistance or not on the herbicides can be identified through detecting the expression quantity of the CYP704B1 gene, and the beckmannia syzigachne CYP704B1 gene has guiding significance ontimely finding out resistance, guiding scientific medication of farmland and relieving the further spread of the drug resistance.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the technical field of genetic engineering, in particular, to the application of the CYP704B1 gene of Smilax chinensis. Background technique

[0002] Long-term continuous and unscientific application of herbicides can easily lead to the emergence of weed resistance. In some areas of my country, wheat and rapeseed have developed serious resistance to herbicides, and with the emergence of non-target resistance, the weed Governance is more difficult. At present, studies have shown that cytochrome P450 can metabolize herbicides into non-toxic substances through dealkylation, ring methyl hydroxylation, hydroxylation of aromatic rings, desulfurization oxidation, and catalyzed ester bond cleavage. , and the increased expression of some genes in the P450 family will make the originally sensitive weed population resistant to herbicides (Yasuor H, Osuna MD, Ortiz A, et al. Mechanism of resistance to penoxsulam in late watergrass[Echinoch...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More