Method for determining stable carbon isotope of volatile compounds in wine based on SPME-GC-IRMS

A technology of volatile compounds and carbon stable isotopes, which is applied in the field of wine detection, can solve the problems of cumbersome operation and achieve the effects of simple operation, simplified isotope determination process, high accuracy and precision

Inactive Publication Date: 2019-06-04
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] In the above-mentioned patents, the main method used is the elemental analysis-isotope mass spectrometry (EA-IRMS) method, and the main problem is that the operation is cumbersome

Method used

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  • Method for determining stable carbon isotope of volatile compounds in wine based on SPME-GC-IRMS
  • Method for determining stable carbon isotope of volatile compounds in wine based on SPME-GC-IRMS
  • Method for determining stable carbon isotope of volatile compounds in wine based on SPME-GC-IRMS

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034]Example 1: Volatile Compound δ in Wine Samples 13 Determination of C value

[0035] Step 1, draw 1.5mL wine sample and place it in the sample bottle for determination.

[0036] Step 2: Aging the CAR / DVB / PDMS extraction head, the aging temperature is 270°C, and the aging time is 30 minutes.

[0037] Step 3: Add a stirring bar to the sample bottle containing the wine sample, tighten the bottle cap with a silicone pad, place the sample bottle in a water bath at 40°C and stir for 15 minutes; insert the CAR / DVB / PDMS extraction head , the extraction temperature was kept at 40° C., and the extraction time was 40 min. At this point the volatile compounds in the wine are adsorbed onto the surface of the extraction head.

[0038] Step 4, confirm that the working environment, air tightness, and vacuum degree of the ion source of the isotope mass spectrometer meet the requirements, and the stability and linearity of the instrument meet the requirements. Use the isotope standard I...

Embodiment 2

[0044] Example 2: Volatile compounds δ in four wine samples 13 Determination of C value

[0045] Step 1: Take wine samples from four different sources, S1, S2, S3, and S4, and draw 1.5mL wine samples sequentially and place them in the sample bottle for determination.

[0046] Step 2: Aging the CAR / DVB / PDMS extraction head, the aging temperature is 270°C, and the aging time is 30 minutes.

[0047] Step 3: Add a stirring bar to the sample bottle containing the wine sample, tighten the bottle cap with a silicone pad, place the sample bottle in a water bath at 40°C and stir for 15 minutes; insert the CAR / DVB / PDMS extraction head , the extraction temperature was kept at 40° C., and the extraction time was 40 min. At this point the volatile compounds in the wine are adsorbed onto the surface of the extraction head.

[0048] Step 4, confirm that the working environment, air tightness, and vacuum degree of the ion source of the isotope mass spectrometer meet the requirements, and t...

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Abstract

The invention relates to a method for determining stable carbon isotope of volatile compounds in a wine based on SPME-GC-IRMS. The method is characterized in that volatile compounds in the wine are extracted through a solid phase microextraction technology, and the compounds are separated by a gas chromatography column; and isotope mass spectrometry is used to determine the delta 13C value in CO2produced by the combustion of the volatile compounds. According to the invention, the solid phase microextraction technology is used to extract the volatile compounds in a wine sample, and GC-IRMS isused to determine the delta 13C value of individual compounds; compared with a method of using EA-IRMS to determine the delta 13C value of ethanol in the wine, the method provided by the invention does not require pretreatment, has the advantage of simple and convenient operation, and is free of an organic solvent; the delta 13C value of volatile materials is accurately and precisely determined; isotope fractionation is prevented; and a useful new method is provided for wine authenticity identification.

Description

technical field [0001] The invention belongs to the technical field of wine detection, in particular to a method for measuring stable carbon isotopes of volatile compounds in wine based on SPME-GC-IRMS. Background technique [0002] Wine is a healthy alcoholic beverage that is deeply loved by consumers. However, the problem of wine counterfeiting is serious (wine varieties, origin counterfeiting, etc.), and it has not been effectively solved yet. Therefore, it is very important to establish fast and effective technical means to ensure the authenticity of wine. [0003] Currently commonly used techniques include gas chromatography (GC), high performance liquid chromatography (HPLC), nuclear magnetic resonance spectroscopy (NMR), inductively coupled plasma atomic emission spectroscopy (ICP-AES), infrared spectroscopy (IR) and isotope ratio mass spectrometry. (IRMS), etc. Among these methods, IRMS is by far the method that can best guarantee the authenticity of wine. The cur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 吴时敏金新宇
Owner SHANGHAI JIAO TONG UNIV
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