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Polyethylene-hydroxyapatite micro-nano multi-level structure composite microsphere material and its application

A technology of hydroxyapatite and polyethylene microspheres, applied in the field of biomedical engineering, can solve the problems of uncontrollable embryonic stem cell differentiation, teratoma, non-localization, etc., achieve good differentiation potential, promote proliferation, and controllable morphology Effect

Active Publication Date: 2021-11-02
UNIV OF JINAN
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

(2) Although embryonic stem cells can differentiate into various cell types, this differentiation is "non-localized". At present, it is not possible to control the differentiation of embryonic stem cells into corresponding cells in specific parts. The current practice can easily lead to teratomas ; and the above-mentioned problems do not exist in the application of adult stem cells
The methods for obtaining hydroxyapatite mainly include hydrothermal method, chemical deposition method, sol-gel method, etc. Among them, the hydrothermal method requires high temperature and high pressure conditions; the sol-gel method requires alkaline conditions and high temperature treatment; these methods Neither are suitable for growing hydroxyapatite on polyethylene microspheres
[0006] In addition, microspheres are currently mainly used in the fields of drug sustained release, cell scaffolding, and cell injection. There are no reports on the use of microspheres to promote the proliferation of adult stem cells and maintain the stemness of adult stem cells.

Method used

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  • Polyethylene-hydroxyapatite micro-nano multi-level structure composite microsphere material and its application
  • Polyethylene-hydroxyapatite micro-nano multi-level structure composite microsphere material and its application
  • Polyethylene-hydroxyapatite micro-nano multi-level structure composite microsphere material and its application

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preparation example Construction

[0030] In one embodiment of the present invention, the preparation method (preparation flow process such as figure 1 shown), including the following steps:

[0031] Based on polyethylene microspheres, hydroxyapatite nanostructures are synthesized on the surface; specifically:

[0032] Soak polyethylene microspheres in calcium nitrate and ammonium dihydrogen phosphate solution, under the condition of stirring and constant temperature water bath at 40-70°C, adjust the pH value of the solution to be alkaline by adding ammonia water dropwise, and collect the microspheres after 1-5 days of reaction. Washed three times with deionized water, then with absolute ethanol, and dried in vacuum to obtain polyethylene microspheres coated with hydroxyapatite nanostructures (such as figure 2 shown).

[0033] Due to its good biocompatibility, hydroxyapatite is currently widely used in tissue engineering, especially in orthopedic research. The methods for obtaining hydroxyapatite mainly inc...

Embodiment 1

[0039]Example 1: Preparation of polyethylene-hydroxyapatite micronano hierarchical structure composite microsphere material

[0040] Immerse polyethylene microspheres (microsphere diameter 50 μm) in the calcium nitrate-ammonium dihydrogen phosphate mixture (the concentration ratio of calcium nitrate and ammonium dihydrogen phosphate is 4:3), adjust the pH to 9 by adding drops, and store at 55 °C Stirring and reacting in a constant temperature water bath for 3 days, the microspheres were harvested, washed three times with deionized water, and then washed with absolute ethanol, and then vacuum-dried to prepare the polyethylene-hydroxyapatite micro-nano multi-level structure composite microsphere material.

Embodiment 2

[0041] Example 2: Preparation of polyethylene-hydroxyapatite micro-nano hierarchical structure composite microsphere material

[0042] Immerse polyethylene microspheres (microsphere diameter 400 μm) in the calcium nitrate-ammonium dihydrogen phosphate mixture (the concentration ratio of calcium nitrate and ammonium dihydrogen phosphate is 5:3), adjust the pH to 10 by dropping, and store at 40 °C Stirring and reacting in a constant temperature water bath for 5 days, the microspheres were harvested, washed three times with deionized water, and then washed with absolute ethanol, and then vacuum-dried to prepare the polyethylene-hydroxyapatite micronano multi-level structure composite microsphere material.

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Abstract

The invention discloses a polyethylene-hydroxyapatite micro-nano multi-level structure composite microsphere material and its application. The polyethylene-hydroxyapatite micro-nano multi-level structure composite microsphere material of the present invention is made of polyethylene microspheres Immerse in calcium nitrate-ammonium dihydrogen phosphate mixture, adjust the pH to 8-10, stir and react in a constant temperature water bath at 40-70°C for 1-5 days, collect microspheres, wash, and dry to obtain surface-coated hydroxyapatite Nanostructured polyethylene composite microspheres. The microsphere has a stable structure, uniform size, a diameter of 50-400 μm, and a hydroxyapatite nanoparticle length of 20-100 nm. Seeding human adipose-derived mesenchymal stem cells on the microspheres can promote the proliferation of stem cells and maintain the stemness of stem cells.

Description

technical field [0001] The invention relates to the technical field of biomedical engineering, in particular to a polyethylene-hydroxyapatite micro-nano multi-level structure composite microsphere material and its application in maintaining stem cell stemness. Background technique [0002] Stem cell therapy technology has shown great clinical application prospects in gene therapy, autoimmune diseases, brain and nervous system diseases, and cardiovascular diseases. Among them, the advantages of autologous adult stem cell transplantation are particularly prominent. [0003] Adult stem cells refer to undifferentiated cells present in an already differentiated tissue. Under certain conditions, adult stem cells either produce new stem cells, or differentiate according to a certain program to produce various specific cell types. Compared with embryonic stem cells, adult stem cells have many advantages, for example: (1) Since the major histocompatibility complex (MHC) of each ind...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J7/14C12N5/0775C08L23/06
Inventor 任娜孙春辉刘宏甘宜梧刘振梁娜谢清华张瑞彤刘佳丽
Owner UNIV OF JINAN