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Method for efficiently creating heterooctaploid silver crucian carp

An octaploid, allo-octaploid technology, applied in fish farming, application, climate change adaptation, etc., can solve the problem of large workload of screening and identification of allo-octaploid, and achieve convenient operation and less blood collection. , easy to grasp effect

Active Publication Date: 2019-06-14
INST OF AQUATIC LIFE ACAD SINICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since the heterologous sperm enters the gibel carp egg, it does not decondense and is in a pyknotic state, so the spontaneous formation of allo-octoploid progenies through heterosperm gynogenesis is only 0.2-0.33%, which also leads to The workload of screening and identifying allo-octoploids in breeding is very heavy

Method used

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  • Method for efficiently creating heterooctaploid silver crucian carp

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Experimental program
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Effect test

Embodiment 1

[0045] Step 1, prepare for going down the pond: in winter, before the parent (variety: heterogeneous gibel carp A+ line) goes down the pond, carry out dry pond disinfection to the parent's cultivation pond, kill miscellaneous fish, then pour new water into the parent's cultivation pond again, At the same time, an isolation net is set at the water inlet of the parent breeding pond to prevent miscellaneous fish from entering the breeding pond;

[0046] Step 2, parental cultivation: male and female screening is carried out before the parents go to the pond, and male fish are eliminated; after the beginning of spring, the water depth of the parental cultivation pond is increased to 1.8 meters;

[0047] The amount of feed to be fed during parental breeding is calculated as 3% of the total body weight of the parents;

[0048] Sprinkle decomposed organic fertilizer 4000kg / hm each time in the parental cultivation pond during parental cultivation 2 , reduce dissolved oxygen in water t...

Embodiment 2

[0052] Step 1, prepare for going down the pond: in winter, before the parent (variety: heterogeneous gibel carp A+ line) goes down the pond, carry out dry pond disinfection to the parent's cultivation pond, kill miscellaneous fish, then pour new water into the parent's cultivation pond again, At the same time, an isolation net is set at the water inlet of the parent breeding pond to prevent miscellaneous fish from entering the breeding pond;

[0053] Step 2, parental cultivation: male and female screening is carried out before the parents go to the pond, and male fish are eliminated; after the beginning of spring, the water depth of the parental cultivation pond is increased to 1.5 meters;

[0054] The amount of feed to be fed during the breeding of the parents is calculated as 2% of the total body weight of the parents;

[0055] Sprinkle decomposed organic fertilizer 3000kg / hm each time into the parental cultivation pond during parental cultivation 2 , reduce dissolved oxyge...

Embodiment 3

[0059] Step 1, prepare for going down the pond: in winter, before the parent (variety: heterogeneous gibel carp A+ line) goes down the pond, carry out dry pond disinfection to the parent's cultivation pond, kill miscellaneous fish, then pour new water into the parent's cultivation pond again, At the same time, an isolation net is set at the water inlet of the parent breeding pond to prevent miscellaneous fish from entering the breeding pond;

[0060] Step 2, parental cultivation: male and female screening is carried out before the parents go to the pond, and the male fish are eliminated; after the beginning of spring, the water depth of the parental cultivation pond is increased to 2.0 meters;

[0061] The feeding amount of the feed during parental breeding is calculated according to 4% of the total body weight of the parents;

[0062] Sprinkle fermented EM bacteria solution 1200 ml / mu / 10 days in the parental cultivation pond during the parental cultivation process to reduce t...

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Abstract

The invention relates to a method for efficiently creating heterooctaploid silver crucian carp. The method comprises an artificial insemination stage, wherein the artificial insemination stage comprises the following steps: preparing a trypsin : adding trypsin powder into a sperm preservation solution, and uniformly mixing to obtain the trypsin solution;adding 0.9-1.1 g of trypsin powder into every 100 mL of sperm preservation solution; treating semen: adding mature semen of the white crucian carp into the trypsin solution, and treating for 13-17 min at the temperature of 22-24 DEG C; insemination hatching: performing dry insemination on the treated semen and the mature eggs of the heterogenetic silver crucian carp, and hatching the obtained fertilized eggs to obtain the heterooctaploid silver crucian carp. The method comprises the following steps: treating spermatozoa by using trypsin solution dissolved in sperm preservation solution to decoagulate the spermatozoa so as to achieve thesuccessful integration of heterologous spermatozoa into the eggs of the heterogenous silver-bearing crucian carp to form heterologous octaploid; further obtaining the heterologous octaploid silver crucian carp with high proportion by setting proper trypsin concentration and treatment time.

Description

technical field [0001] The invention belongs to the field of fish genetics and breeding, and in particular relates to a method for efficiently creating heterologous octoploid gibel carp. Background technique [0002] Hybridization and polyploidy can provide the original driving force and material basis for the evolution of species and the formation of excellent traits. Theoretically, cell size tends to increase due to increased ploidy of the genome, and polyploids often exhibit faster growth and larger vegetative organs or individuals than their diploid relatives. As a classic and effective cell engineering breeding technology, polyploid breeding has been widely used in animal and plant breeding, including breeding of fish and shellfish species. In aquaculture animals, successful examples are triploid Pacific oysters and auto- or allopolyploids synthesized from naturally polyploid fish such as Cyprinidae and Salmonidae. Therefore, optimizing or developing new and efficient...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10
CPCY02A40/81
Inventor 李志桂建芳汪洋周莉王忠卫李熙银张晓娟鲁蒙余鹏
Owner INST OF AQUATIC LIFE ACAD SINICA
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