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Processing method and application of low-antigen soybean pulp

A technology of low-antigen soybean meal and processing method, which is applied in the application, food processing, animal feed and other directions, can solve the problems of reducing the antigenicity of soybean meal and the difference in the degradation effect of antigenic proteins, and achieves good promotion and application prospects and reduces the effect of antigenicity.

Inactive Publication Date: 2019-06-14
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, natural soybean protein molecules are highly compressed, compact in structure, and have strong resistance to enzymatic hydrolysis
Therefore, the direct microbial fermentation and enzymatic hydrolysis technology to reduce the antigenicity of soybean meal is limited
In addition, for different strains, due to the different secreted proteases in the fermentation process, their degradation effects on antigenic proteins are also different.

Method used

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  • Processing method and application of low-antigen soybean pulp

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The effect of stress on reducing soybean meal antigens, as follows:

[0040] 1) Remove impurities from the soybean meal, crush it, and sieve it through a 100-mesh sieve, and take the under-sieve as soybean meal powder.

[0041] 2) According to the material-to-liquid ratio of 1g:10mL, take soybean meal powder and water, mix them evenly to make a soybean meal suspension, vacuum-seal the soybean meal suspension in a two-layer polypropylene plastic bag, and then put it into a pressure vessel. Under 500 MPa, ultra-high pressure treatment for 30 minutes.

[0042] 3) Transfer the high-pressure treated soybean meal suspension to a steam pot, cook at 100° C. for 20 minutes, and cool to room temperature after cooking.

[0043] 4) Preparation of a mixed solution of composite bacteria: Bacillus subtilis, Bacillus licheniformis and Bifidobacterium longum were respectively activated so that the concentration of the bacteria solution was 2×10 8 cfu / mL. According to the inoculum rat...

Embodiment 2

[0050] The effect of ultra-high pressure treatment time on the reduction of soybean meal antigens is as follows:

[0051]1) Remove impurities from the soybean meal, crush it, and sieve it through a 100-mesh sieve, and take the under-sieve as soybean meal powder.

[0052] 2) According to the material-to-liquid ratio of 1g:10mL, take soybean meal powder and water, mix them evenly to make a soybean meal suspension, vacuum-seal the soybean meal suspension in a two-layer polypropylene plastic bag, and then put it into a pressure vessel. Under high pressure treatment for 5 to 30 minutes.

[0053] 3) Transfer the high-pressure treated soybean meal suspension to a steam pot, cook at 100° C. for 20 minutes, and cool to room temperature after cooking.

[0054] 4) Preparation of the mixed solution of composite bacteria: respectively activate Bacillus subtilis, Bacillus licheniformis and Bifidobacterium longum, so that the concentration of the bacteria solution is 2×10 8 cfu / mL. Accord...

Embodiment 3

[0061] The effect of bacterial strain screening on reducing soybean meal antigens is as follows:

[0062] 1) Remove impurities from the soybean meal, crush it, and sieve it through a 100-mesh sieve, and take the under-sieve as soybean meal powder.

[0063] 2) According to the material-to-liquid ratio of 1g:10mL, take the sieved soybean meal powder and water, mix them evenly to make a soybean meal suspension, vacuum-seal the soybean meal suspension in a two-layer polypropylene plastic bag, and then put it into a pressure vessel. Under the pressure of 100 MPa, the ultra-high pressure treatment was performed for 30 minutes.

[0064] 3) Transfer the high-pressure treated soybean meal suspension to a steam pot, cook at 100° C. for 20 minutes, and cool to room temperature after cooking.

[0065] 4) Bacillus subtilis, Bacillus licheniformis, Bifidobacterium longum, Lactobacillus plantarum, Saccharomyces cerevisiae, Lactobacillus casei, Saccharomyces cerevisiae, Aspergillus oryzae an...

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Abstract

The invention relates to a processing method and application of low-antigen soybean pulp. The processing method comprises the following steps of: 1) pretreating bean pulp to obtain bean pulp powder; 2) uniformly mixing pretreated bean pulp powder and water to prepare a bean pulp suspension, sealing the bean pulp suspension in a polypropylene plastic bag in vacuum, putting the bag in a pressure container, and carrying out high-pressure treatment; 3) cooking high-pressure treated bean pulp suspension, and cooling to room temperature; 4) transferring cooked bean pulp suspension into a fermentation tank, adding a mixed solution of composite strains of bacillus subtilis, bacillus licheniformis and bifidobacteriumlongum, and fermenting at 30-37 DEG C for 12-48 hours; and 5) carrying out low-temperature stirring, drying, crushing, and cooling fermented bean pulp to obtain the low-antigen bean pulp. By the adoption of the processing method and application of the low-antigen soybean pulp, ultra-high pressure and multi-strain fermentation technology is adopted, which is a green, safe and nuisance-free production technology; and the obtained low-antigen soybean pulp is particularly suitable for feeding daily ration of young animals, and has good popularization and application prospects .

Description

technical field [0001] The invention belongs to the technical field of agricultural and sideline products processing, and in particular relates to a processing method and application of low-antigen soybean meal fermented by ultra-high pressure and multi-bacteria. Background technique [0002] Soybean meal is a by-product obtained after extracting soybean oil from soybeans. Its protein content is 43% to 46%, and it is rich in biologically active substances such as isoflavones and phospholipids, as well as nutrients such as fat, sugar, and minerals. It is a very high-quality food Vegetable protein feed raw materials for livestock and poultry. However, due to the antigenic protein contained in soybean meal, it will cause allergic reactions in young animals, with symptoms such as diarrhea, enteritis, growth stagnation, etc., and even death of animals, which limits its application in young animal feed to a certain extent. Therefore, reducing the activity of antigenic protein in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/18A23K10/37
CPCY02P60/87
Inventor 杨慧武俊瑞李苏红
Owner SHENYANG AGRI UNIV
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