Molecular markers for rice cold-tolerant major QTL qCTS12 and identification method and application of molecular markers

A technology of molecular markers and identification methods, applied in the field of molecular genetics, can solve the problems of limited residence and fine-mapping QTL, and achieve the effects of accelerating the breeding process, facilitating cross-breeding, and clear positions

Inactive Publication Date: 2019-06-14
GUANGXI UNIV
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the QTLs are only at the level of initial mapping, and the QTLs for fine mapping and cloning are very limited

Method used

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  • Molecular markers for rice cold-tolerant major QTL qCTS12 and identification method and application of molecular markers
  • Molecular markers for rice cold-tolerant major QTL qCTS12 and identification method and application of molecular markers
  • Molecular markers for rice cold-tolerant major QTL qCTS12 and identification method and application of molecular markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Screening and mapping of molecular markers

[0040] 1. Construction of the cold-tolerant chromosome segment substitution line CSSL12 and analysis of the cold-resistant phenotype of the replaced segment

[0041] (1) Using the cold-tolerant Guangxi common wild rice DP15 as the donor parent and the cold-sensitive rice variety 9311 as the recurrent parent to construct chromosome segment substitution lines (chromosome segment substitution lines, CSSLs), through continuous backcrossing, selfing and orientation Choice, in BC 5 f 2 Two chromosomal segment replacement lines with similar genetic backgrounds, DC90 and NC, were obtained. Most of the genetic background of DC90 is the same as that of the recurrent parent 9311, only the fragment replacement of DP15 on chromosome 3 and chromosome 12, the length of which is 10Mb and 20Mb respectively. However, NC only has a fragment replacement of DP15 on chromosome 3, which is the same as the replacement region of chromosome 3 in DC90...

Embodiment 2

[0063] Validation of Molecular Markers

[0064] 1. Materials and methods

[0065] 1.1 Materials

[0066] 52 rice varieties from different sources

[0067] 1.2 Method

[0068] 1.2.1 PCR amplification: Genomic DNA of rice leaves was extracted by the TPS extraction method, and the extracted sample DNA was amplified with primers M5 and M6, and the method was the same as in Example 1.

[0069] 1.2.2 Identification of cold stress phenotypes: select full-bodied seeds and germinate in water at 30°C for 3 days. After the seeds are "white", they are sown in plastic buckets filled with field soil to grow normally, the ambient temperature is 30°C, the light is 20000 Lux, and the air humidity is 40%. Cold treatment was carried out when the seedlings grew to the 3-leaf stage. When the seedlings grow to the 3-leaf stage, put them into an artificial climate culture room at 8°C-10°C for cold treatment for 5 days, during which the photoperiod is 13h (light) / 11h (dark), the light intensity ...

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Abstract

The invention discloses molecular markers for the rice cold-tolerant major QTL qCTS12 and an identification method and application of the molecular markers. The molecular markers for the rice cold-tolerant major QTL qCTS12 are M5 and M6, PCR amplification is conducted by using primers corresponding to the molecular markers M5 and M6, and an amplified product is detected. The identification methodof the molecular markers M5 and M6 is used in location of the rice cold-tolerant qCTS12. By using the molecular markers M5 and M6, whether there is the cold-tolerant major site in a rice variety or not can be effectively detected, and the efficiency of selecting a cold-tolerant material in the rice seedling stage is quickly improved. By means of the molecular markers, location of the cold-tolerantqCTS12 can be accelerated, and after the molecular markers are used for helping selective breeding, the breeding progress is accerated.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and specifically relates to a molecular marker of rice cold-tolerant main effect QTL qCTS12 and its identification method and application thereof, and is suitable for the molecular marker of rice seedling stage cold-tolerant main effect QTL qCTS12 and its selection of cold-tolerant varieties in rice seedling stage. applications in education and genetic resource screening. Background technique [0002] As one of the most important food crops in the world, rice production is of great strategic significance for solving future global food problems. However, the main rice producing areas in my country often suffer from chilling damage due to low temperature stress during the rice planting period. For example, the Northeast region will encounter chilling damage every 3 to 4 years on average; rotten seedlings of early rice; "cold dew wind" in late rice in the south will cause delayed heading of rice; t...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
Inventor 罗继景岑卫健刘剑镔王永亮赵文龙李容柏
Owner GUANGXI UNIV
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