Application of scutellarin to preparing kidney ischemic reperfusion injury preventing/protecting drugs/drug combinations

A technology of ischemia-reperfusion and baicalin, applied in the field of biomedicine, can solve the problem of lack of strong evidence of drug action

Inactive Publication Date: 2019-06-21
THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the mechanism of acute kidney injury is still inconclusive, so there is still a lack of strong evidence for its drug effects, and it is of great significance for clinical diagnosis and treatment to explore drugs related to acute kidney injury for the pathogenesis

Method used

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  • Application of scutellarin to preparing kidney ischemic reperfusion injury preventing/protecting drugs/drug combinations
  • Application of scutellarin to preparing kidney ischemic reperfusion injury preventing/protecting drugs/drug combinations
  • Application of scutellarin to preparing kidney ischemic reperfusion injury preventing/protecting drugs/drug combinations

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1 scutellarin pretreatment grouping

[0024] Thirty 8-week-old, healthy male SPF Wistar rats weighing 250-300g were housed in isolated cages with independent air supply, the temperature was controlled at 21°C±2°C, the relative humidity was controlled at 50%+15%, and the day and night cycle For 12 hours, eat normally, and change the litter every 2 days. They were randomly divided into 5 groups, namely normal group, sham operation group, AKI group, scutellarin+AKI group, and Saline+AKI group, with 6 rats in each group. Rats in the administration group (scutellarin+AKI group) and the control group (Saline+AKI) were given drug treatment by intraperitoneal injection at the same time every day 7 days before modeling. According to the experimental grouping, scutellarin+AKI The Saline+AKI group and the Saline+AKI group were given scutellarin and an equal volume of normal saline at a dose of 50 mg / kg.d, and the dose was determined by referring to previous experimenta...

Embodiment 2

[0028] Embodiment 2 ischemia-reperfusion model making

[0029]6-8 weeks Wistar rats were cultured at 21°C±2°C, 50%+15% humidity, and 12 hours of light, and were fed with standard pellet feed and sterile water. Rats were kept in a cage of 6 and fasted for 12 hours before surgery. The high-pressure steam sterilizer is set at 120°C for 40 minutes, and the surgical instruments are put in for sterilization. Rats were anesthetized by intraperitoneal injection of pentobarbital sodium (30 mg / kg), and the rats were in a state of complete muscle relaxation, and the anesthesia was successful. The abdomen was placed in a supine position, and the limbs were gently fixed with elastic ropes. La, because the time of anesthesia cannot be determined, the rat may be awake during the operation, and the operator may be bitten if the fixation is not good. Lift the tongue out of the mouth from the side to prevent the animal from suffocating due to the tongue falling back to block the pharynx. Rem...

Embodiment 3

[0031] Embodiment 3 Rat blood creatinine, blood urea nitrogen monitoring

[0032] First anesthetize the animal, and fix it on the surgical rack in a supine position, and incise the abdominal cavity from the midline of the abdomen to expose the inferior vena cava clearly. Use a syringe to insert the blood vessel towards the centrifugal end of the inferior vena cava and suck out the blood. When drawing blood, quickly prevent blood coagulation from blocking the needle tube. Draw 5ml of blood and place it in a 5ml EP tube for 1 hour. Centrifuge at 3000rpm for 15 minutes in a high-speed centrifuge. Transfer the upper serum to a new EP tube for use in blood creatinine and blood urea nitrogen. For detection, store at 4°C. The specimens were sent to the laboratory department of the hospital within 24 hours, and the levels of creatinine and urea nitrogen were detected by enzymatic method. The test results are shown in Table 1.

[0033] Table 1 Rat serum creatinine (μmol / L) result

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Abstract

The invention discloses application of scutellarin to preparing kidney ischemic reperfusion injury preventing / protecting drugs / drug combinations and belongs to the field of biological medicines. The application proves possibility of the preventing / protecting effects of the scutellarin on human ischemic reperfusion-induced acute kidney injury; data show that the content of Nrf2 (nuclear factor erythroid-2-related factor 2) proteins in a kidney ischemic reperfusion model is significantly reduced, and after the scutellarin is applied, expression of Nrf2 genes can have change of statistical significance, and by means of the molecule docking technology, possibility of interaction of scutellarin molecules and the protein structure is testified, and a theoretical foundation for enlarging the possible application range of the scutellarin can be provided. The application of the scutellarin to preparing kidney ischemia reperfusion injury preventing / protecting drugs / drug combinations can be applied to theoretical research and clinical treatment of kidney ischemic reperfusion injury and provide the theoretical foundation for enlarging the possible application range.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the application of scutellarin in the preparation of a drug / medicine composition for preventing / protecting renal ischemia-reperfusion injury. Background technique [0002] Acute kidney injury (Acute Kidney Injury, AKI) is caused by a variety of etiologies and causes a sudden decline in renal function within a short period of time, with a mortality rate as high as 50%-79%. Renal ischemic injury is one of the most common causes of AKI, and it is widely seen clinically in pathological conditions such as surgery, kidney transplantation, and acute cardiac insufficiency. A large number of animal model experiments and human histopathological studies of renal ischemia-reperfusion injury have shown that oxidative stress, inflammation and apoptosis may be the most important pathophysiological changes of ischemic acute kidney injury. [0003] Scutellarin (CAS: 27740-01-8) is a Chines...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P13/12
Inventor 徐岩代杰杨成宇
Owner THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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