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Preparation process of xanthan gum fermentation culture medium

A fermentation medium and a technology for preparation process, applied in the directions of fermentation, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of low yield and high cost of xanthan gum fermentation medium, reduce industrial costs and save Raw material cost, good effect

Inactive Publication Date: 2019-06-25
卢松
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the invention is to solve the defects that the cost of xanthan gum fermentation medium is relatively high and the yield is low in the prior art, and a preparation process for xanthan gum fermentation medium is provided

Method used

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  • Preparation process of xanthan gum fermentation culture medium
  • Preparation process of xanthan gum fermentation culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The substratum that is used for xanthan gum fermentation, it comprises following components:

[0037] Glucose 40g / L, cornstarch 60g / L, bacterial proteolysis solution 50g / L, oleic acid 10g / L, calcium carbonate 3g / L, magnesium sulfate heptahydrate 1g / L, dipotassium hydrogen phosphate 1g / L, yellow rot Acid 20mg / L, VB 1 20mg / L, pH 7.0-7.2.

[0038] The preparation method of described culture medium is:

[0039] Take each raw material, add it to water in turn, stir evenly, adjust the pH, and you get it;

[0040] The preparation method of the thallus enzymolysis liquid is as follows: collect the thallus in the xanthan gum fermentation liquid, dry to dry thalline with water content less than 5wt%, dilute with water to dry thalline concentration is 40g / L, place in high speed Shear at a speed of 10000rpm for 120s in a shearing machine to obtain a bacterial suspension, add the same volume of hydrochloric acid solution with a concentration of 1mol / L to the bacterial suspension...

Embodiment 2

[0043] Utilize the technique of above-mentioned culture medium fermentation to prepare xanthan gum, it comprises the steps:

[0044] According to Xanthomonas ATCC 17915 seed solution (1 x 10 8CFU / mL) according to the inoculum amount of 8% (volume ratio) into the culture medium for fermentation and cultivation, the temperature is 30 ° C, the fermentation and cultivation time is 72 hours, and the fermentation liquid is obtained; The dissolved oxygen level is 20%, and the residual sugar is controlled at not less than 2% by automatic feeding of glucose solution.

Embodiment 3

[0058] One, the influence of the culture medium of embodiment 1 and comparative examples 1-4 on xanthan gum.

[0059] The influence of each culture medium on the fermentation of xanthan gum was analyzed, and the fermentation process was referred to Example 2. The specific data indicators are shown in Table 1:

[0060] Table 1

[0061] index

[0062] Conclusion: Compared with Comparative Example 1, the present invention adopts bacterial proteolysis solution to replace common yeast extract, and adopts cornstarch to replace part of glucose, which has no great influence on the quality of fermentation rubber production, and the rubber yield of the present invention is relatively high. Ratio 1 is also improved to a certain extent, but the cost of fermentation medium is significantly reduced; compared with comparative example 2-4, the present invention is better than comparative example 2-4 in terms of xanthan gum output and quality, as seen, oleic acid and yellow rot Aci...

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Abstract

The invention belongs to the technical field of fermentation and discloses a preparation process of a xanthan gum fermentation culture medium. The preparation proces comprises the following steps: andsequentially adding glucose, corn starch, thallus proteolysis solution, oleic acid, calcium carbonate, magnesium sulfate heptahydrate, dipotassium hydrogen phosphate, fulvic acid and VB1 into water,uniformly stirring, and adjusting the pH value. The culture medium prepared by the process is suitable for producing xanthan gum by fermenting xanthomonas, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a preparation process for a xanthan gum fermentation medium. Background technique [0002] Xanthan gum (Xanthan gum), also known as xanthomonas polysaccharide or xanthan gum, is fermented by Xanthomonas campestris or other strains of Xanthomonas genus with carbohydrates as the main raw material. The biosynthesis mechanism of the high molecular acidic extracellular heteropolysaccharide produced is "non-template synthesis mechanism". Xanthan gum molecules are composed of "pentasaccharide repeating unit" structural polymers. Xanthan gum molecules form a double-strand secondary helical three-dimensional structure by hydrogen bonds, and the double-strand helix structures are combined by weak non-covalent bonds. In a very dilute (< 1 %) aqueous solution, xanthan gum presents a typical four-stage chrysanthemum-like aggregation structure without heating. [0003] Xa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/06C12N1/20C12R1/64
Inventor 刘超伏广好许传娟李文全高启超位凤宇
Owner 卢松
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