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Method for preparing deep fermented feed containing two high-content organic acids

A production method and deep fermentation technology, applied in animal feed, animal feed, bacteria used in food preparation, etc., can solve the problem of high price, easy loss of L-malic acid, and ineffective guarantee of product quality stability To achieve stable product quality, improve the health level of the digestive tract, and reduce biological activity

Inactive Publication Date: 2019-07-05
佛山播恩生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, L-malic acid is easily lost during processing, storage and use due to its monomer or pure product, the stability of product quality cannot be effectively guaranteed, and the price is high

Method used

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  • Method for preparing deep fermented feed containing two high-content organic acids
  • Method for preparing deep fermented feed containing two high-content organic acids
  • Method for preparing deep fermented feed containing two high-content organic acids

Examples

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Effect test

preparation example Construction

[0059] Preparation of monospore suspension: Take the inclined plane, add 6mL, 0.1mol / L, pH 6.0 phosphate buffer, scrape off the spores with an inoculation loop, shake the test tube, and immediately filter through a funnel with filter paper to obtain a monospore suspension , with a spore concentration of 10 6 individual / mL.

[0060] (1) Preparation of nitrosoguanidine solution

[0061] Weigh 2 mg with an analytical balance, add 2 mL of 0.1 mol / L, pH 6.0 phosphate buffer, add a little acetone appropriately to help dissolve nitrosoguanidine, and shake to dissolve in the dark.

[0062] (2) Mutagenesis treatment

[0063] Draw 1mL of nitrosoguanidine solution, add 1mL of spore suspension, shake at 30°C for 30min, immediately dilute 1000 times with cold normal saline to stop the action, and then dilute with 10 -2 、10 -3 、10 -4 Three dilutions were separated and cultured, and counted after 3 days at 30°C.

[0064] (3) Select the colony culture after the mutation according to the...

Embodiment 1

[0069] An embodiment of the deep fermented feed containing two kinds of high-content organic acids described in the present invention, the fermented feed described in this embodiment is prepared by the following method:

[0070] (1) Using the above-mentioned mutagenesis breeding method for Penicillium funiculosa to obtain a mutant strain with high yield of malic acid, which is fermented under ventilation and stirring conditions to obtain a Penicillium funiculum fermentation liquid;

[0071] (2) The culture solution of Lactobacillus casei is mixed with the concentrated MRS medium of the same volume to form a lactic acid bacteria fermentation solution, which is then mixed with a certain volume of Penicillium fungus fermentation solution after fermentation for a certain period of time , continue to carry out static anaerobic fermentation in the tank, and obtain a mixed fermentation broth rich in L-malic acid and L-lactic acid and containing high concentration of lactic acid bacter...

Embodiment 2

[0076] An embodiment of the deep fermented feed containing two kinds of high-content organic acids described in the present invention, the fermented feed described in this embodiment is prepared by the following method:

[0077] (1) Using the above-mentioned mutagenesis breeding method for Penicillium funiculosa to obtain a mutant strain with high yield of malic acid, which is fermented under ventilation and stirring conditions to obtain a Penicillium funiculum fermentation liquid;

[0078] (2) The culture solution of Lactobacillus casei is mixed with the same volume of concentrated MRS medium to form a lactic acid bacteria fermentation solution, which is then mixed with the same volume of Penicillium fungus fermentation solution after fermentation for a certain period of time , continue to carry out static anaerobic fermentation in the tank, and obtain a mixed fermentation broth rich in L-malic acid and L-lactic acid and containing high concentration of lactic acid bacteria af...

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Abstract

The invention discloses a method for preparing a deep fermented feed containing two high-content organic acids. The method comprises the following steps: carrying out liquid culture on four microorganisms separately in a mixed manner to form a composite bacterial solution, inoculating the composite bacterial solution to a feed substrate, and fermenting and storing the feed. The microorganisms arelactobacillus casei, candida utilis, penicillium tetani and bacillus subtilis. According to the invention, L-lactic acid and L-malic acid are directionally induced by four fermentation microorganismsto prepare the deep fermented feed with high content of L-lactic acid and L-malic acid, so that the product quality of the fermented feed is stable and safe, and the health level, biological activityand cost of the digestive tract of livestock and poultry can be greatly improved.

Description

technical field [0001] The invention relates to the technical field of feed, in particular to an animal fermented feed and a preparation method thereof. Background technique [0002] With the rapid development of modern animal husbandry, my country's feed resources are becoming increasingly scarce, and the contradiction of rising breeding costs is becoming more and more prominent. At the same time, due to the abuse of antibiotics in the breeding industry in the past two decades, pathogenic bacteria in animals have developed serious drug resistance, the disease resistance of animals has decreased, and the risk of various diseases has also increased. The use of antibiotics leads to drug residues in animal products, which poses a great threat to food safety. For this reason, my country's Ministry of Agriculture and Rural Affairs has officially announced that the use of antibiotics in feed will be completely banned in 2020. Therefore, the development of low-cost, high digestio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12
CPCA23K10/12A23V2400/125
Inventor 倪冬姣邹新华许赣荣曾李王志博张增玉邓琳陆东东郭丽向江波卢秋咏温志斌张艺伟
Owner 佛山播恩生物科技有限公司
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