Preparation method of mannose oligosaccharides

A technology for oligomannose and galactomannose, which is applied in the field of preparation of mannose oligosaccharides, can solve the problems of low yield of mannose oligosaccharides, low selectivity of mannose oligosaccharides, serious corrosion of inorganic acid equipment, etc. , to achieve the effect of uniform hydrolysis and fast hydrolysis

Active Publication Date: 2019-07-09
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The acid hydrolysis method has high efficiency and high substrate concentration will not inhibit the reaction, but the inorganic acid will seriously corrode the equipment and the selectivity of oligomannose is low
In the existing process of acid hydrolysis to produce mannose oligosaccharides, most of the acids used are sulfuric acid and hydrochloric acid, which are severely corrosive to equipment, and the yield of mannose oligosaccharides is low.

Method used

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  • Preparation method of mannose oligosaccharides
  • Preparation method of mannose oligosaccharides
  • Preparation method of mannose oligosaccharides

Examples

Experimental program
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Embodiment 1

[0033] This embodiment firstly provides the preparation method of galactomannan, comprising: taking 6g of industrial honey locust polysaccharide gum powder, hydrating with 300g of 70°C for 3h, centrifuging to remove water-insoluble matter, and then taking the supernatant in a ratio of 1:1 (v / v) Add absolute ethanol under stirring, centrifuge after standing for 2 hours, and the precipitate is galactomannan. The supernatant can be distilled under reduced pressure to recover ethanol and water.

[0034] This embodiment also provides a preparation method of oligomannose, comprising: adding water to the galactomannan prepared in this embodiment to make a solution with a concentration of 10%, and homogenizing it at 200 MPa by a high-pressure fluid nano-homogenizer, homogenizing The quality-treated galactomannan gum was transferred into a reaction kettle, 20mL of 5M acetic acid was added, and reacted at 130°C for 2 hours to obtain an acetic acid degradation liquid; the degradation li...

Embodiment 2

[0038] This example provides a preparation method of mannose oligosaccharides. The difference from Example 1 is that the galactomannan after high-pressure fluid nano-homogenization treatment is moved into the reaction kettle, 20mL of 6M acetic acid is added, and the reaction is carried out at 120°C for 2h , to obtain acetic acid degradation solution; the degradation solution is vacuum concentrated to recover acetic acid, and further spray-dried to obtain mannose oligosaccharides.

[0039] The acetic acid hydrolyzate obtained in the present embodiment is analyzed by high performance liquid chromatography, wherein the total concentration of mannose oligosaccharides (mannobiose, mannotriose, mannotetraose) is 32.7g / L, and wherein mannose is 10.4g / L L, mannotriose is 11.7g / L, and mannotetraose is 10.6g / L.

[0040] The total sugar content in the mannose oligosaccharide product prepared in this embodiment was 93.3%.

Embodiment 3

[0042] This example provides a preparation method of mannose oligosaccharides. The difference from Example 1 is that the galactomannan after high-pressure fluid nano-homogenization treatment is moved into a reaction kettle, 20mL of 6M acetic acid is added, and the reaction is carried out at 130°C for 1 hour. Acetic acid degradation solution is obtained; the degradation solution is vacuum concentrated to recover acetic acid, and further spray-dried to obtain mannose oligosaccharides.

[0043] The acetic acid hydrolyzate obtained in the present embodiment is analyzed by high performance liquid chromatography, wherein the total concentration of mannose oligosaccharides (mannobiose, mannotriose, mannotetraose) is 30.5g / L, and wherein mannobiose is 9.6g / L L, mannotriose is 11.3g / L, mannotetraose is 9.6g / L.

[0044] The total sugar content in the mannose oligosaccharide product prepared in this example was 93.1%.

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Abstract

The invention relates to a preparation method of mannose oligosaccharides. The method comprises the following steps: performing high-pressure fluid nano homogenization treatment on a galactomannan solution, adding acetic acid, and performing a degradation reaction; or, the method further comprises the steps of after the degradation reaction is completed, performing reduced-pressure concentration to recover the acetic acid, and performing spray drying on the concentrate to obtain the mannose oligosaccharides. According to the method provided by the invention, the galactomannan is subjected to homogenization by a high-pressure fluid nano homogenizer, the acetic acid is directly added for hydrolysis, so that the hydrolysis is uniform, and the hydrolysis speed is fast; compared with inorganicacids such as hydrochloric acid, sulfuric acid and trifluoroacetic acid, the acetic acid belongs to organic acids, has less corrosion to equipment and is easy to recycle; and the method can improve the production efficiency of the mannose oligosaccharides and improve the selectivity of the product, and has good industrial application prospects.

Description

technical field [0001] The invention relates to a preparation method of oligomannose. Background technique [0002] Galactomannan colloids mostly exist in the endosperm of legume seeds, which are rich in resources, have good water solubility and still have high viscosity in dilute solutions. However, due to the high viscosity of galactomannan, its application in food and medicine is limited. Therefore, it is necessary to degrade and modify galactomannan to realize its high-value utilization. Studies have shown that the main chain of galactomannan molecular structure is composed of D-mannopyranose linked by β-(1→4)-glycosidic bonds, and D-galactose is passed through α-(1→6) in the form of side chains. - Glycosidic linkages to the mannose backbone. [0003] Mannan oligosaccharides are made with galactomannans (C 6 h 10 o 5 ) n (n represents the average degree of polymerization) The raw material is an oligosaccharide prepared by a series of methods. Mannose oligosacchar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C13K13/00C08B37/00
CPCC13K13/00C08B37/0003C08B37/0087
Inventor 蒋建新徐伟刘彦涛张威伟
Owner BEIJING FORESTRY UNIVERSITY
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