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A kind of preparation method of mannose oligosaccharide

A technology of oligomannose and galactomannan, which is applied in the field of preparation of oligomannose, can solve the problems of low selectivity of oligomannose, serious corrosion of inorganic acid equipment, and low yield of oligomannose , to achieve the effect of short reaction time, fast hydrolysis speed and increased yield

Active Publication Date: 2022-06-28
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The acid hydrolysis method has high efficiency and high substrate concentration will not inhibit the reaction, but the inorganic acid will seriously corrode the equipment and the selectivity of oligomannose is low
In the existing process of acid hydrolysis to produce mannose oligosaccharides, most of the acids used are sulfuric acid and hydrochloric acid, which are severely corrosive to equipment, and the yield of mannose oligosaccharides is low.

Method used

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  • A kind of preparation method of mannose oligosaccharide
  • A kind of preparation method of mannose oligosaccharide
  • A kind of preparation method of mannose oligosaccharide

Examples

Experimental program
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Embodiment 1

[0033] This example first provides a method for preparing galactomannan, which includes: taking 6 g of industrial acacia polysaccharide gum powder, hydrating it with 300 g at 70°C for 3 hours, centrifuging to remove water-insoluble matter, and then taking the supernatant at a ratio of 1:1 (v / v) Add absolute ethanol under stirring, stand for 2 hours and then centrifuge, and the precipitate is galactomannan. The supernatant can be distilled under reduced pressure to recover ethanol and water.

[0034] This embodiment also provides a method for preparing mannose oligosaccharide, which includes: adding water to the galactomannan prepared in this embodiment to make a solution with a concentration of 10%, and homogenizing it at 200 MPa through a high-pressure fluid nano-homogenizer to homogenize it. The quality-treated galactomannan gum was transferred into the reaction kettle, 20 mL of 5M acetic acid was added, and the reaction was carried out at 130 °C for 2 h to obtain an acetic...

Embodiment 2

[0038] This example provides the preparation method of oligomannose, and the difference from Example 1 is only that the galactomannan treated by high-pressure fluid nano-homogenization is moved into the reaction kettle, 20 mL of 6M acetic acid is added, and the reaction is carried out at 120 ° C for 2 h , to obtain an acetic acid degradation solution; the degraded solution is concentrated in a vacuum to recover acetic acid, and then spray-dried to obtain mannose oligosaccharides.

[0039] The acetic acid hydrolyzate obtained in the present embodiment is analyzed by high performance liquid chromatography, wherein the total concentration of oligomannose (mannobiose, mannotriose, mannotetraose) is 32.7g / L, and wherein mannobiose is 10.4g / L. L, mannotriose was 11.7g / L, and mannotetraose was 10.6g / L.

[0040] The total sugar content in the mannose oligosaccharide product prepared in this example is 93.3%.

Embodiment 3

[0042] This example provides the preparation method of oligomannose, the difference from Example 1 is only that the galactomannan treated by high pressure fluid nano-homogenization is moved into the reaction kettle, 20 mL of 6M acetic acid is added, and the reaction is carried out at 130 ° C for 1 h, An acetic acid degradation solution is obtained; the degradation solution is concentrated in a vacuum to recover acetic acid, and is further spray-dried to obtain mannose oligosaccharides.

[0043] The acetic acid hydrolyzate obtained in the present embodiment is analyzed by high performance liquid chromatography, wherein the total concentration of oligomannose (mannobiose, mannotriose, mannotetraose) is 30.5g / L, and wherein mannobiose is 9.6g / L. L, mannotriose was 11.3g / L, and mannotetraose was 9.6g / L.

[0044] The total sugar content in the mannose oligosaccharide product prepared in this example is 93.1%.

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Abstract

The invention relates to a preparation method of mannose oligosaccharides, which comprises: performing high-pressure fluid nano-homogeneous treatment on a galactomannan solution, and then adding acetic acid to perform a degradation reaction; or further comprising concentrating under reduced pressure after the degradation reaction is completed Acetic acid is recovered, and the concentrated solution is spray-dried to obtain mannose oligosaccharides. In the invention, the galactomannan is homogenized by a high-pressure fluid nanometer homogenizer, and then directly hydrolyzed by adding acetic acid, so that the hydrolysis is uniform and the hydrolysis speed is fast. Compared with inorganic acids such as hydrochloric acid, sulfuric acid, and trifluoroacetic acid, acetic acid is an organic acid, which is less corrosive to equipment and easy to recycle. The method of the invention can improve the production efficiency of the mannose oligosaccharide and product selectivity, and has good industrial application prospects.

Description

technical field [0001] The present invention relates to a preparation method of oligomannose. Background technique [0002] Galactomannan colloids mostly exist in the endosperm of legume seeds, which are rich in resources, have good water solubility and still have high viscosity in dilute solutions. However, its application in food and medicine is limited due to the high viscosity of galactomannan. Therefore, it is necessary to degrade and modify galactomannan to realize its high-value utilization. Studies have shown that the main chain of galactomannan is composed of D-mannose linked by β-(1→4)-glycosidic bonds, and D-galactose passes through α-(1→6) in the form of side chains. - Glycosidic bonds are attached to the mannose backbone. [0003] Mannose oligosaccharides containing galactomannan (C 6 H 10 O 5 ) n (n represents the average degree of polymerization) raw material An oligosaccharide prepared by a series of methods. Mannose oligosaccharide is a functional ol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C13K13/00
CPCC13K13/00C08B37/0003C08B37/0087
Inventor 蒋建新徐伟刘彦涛张威伟
Owner BEIJING FORESTRY UNIVERSITY
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