Preparation method and application of zein-polysaccharide composite particle

[0027] Example 1

[0028] Prepare prolamin-polysaccharide composite particles according to the following steps:

[0029] (1) Add 0.1 g of zein to 50 mL of ethanol aqueous solution (the volume fraction of ethanol is 60%), and stir at room temperature until fully dissolved to obtain an ethanol aqueous solution of zein;

[0030] (2) Disperse 0.005 g of propylene glycol alginate (the mass ratio of zein to propylene glycol alginate is 20:1) in 50 mL of ethanol aqueous solution (the volume fraction of ethanol is 60%), magnetically stir until completely dissolved, and obtain Aqueous solution of propylene glycol alginate in ethanol;

[0031] (3) Add the zein ethanol aqueous solution obtained in step (1) to the ethanol aqueous solution of propylene glycol alginate obtained in step (2) under stirring conditions, and stir for 2 hours to obtain a zein-propylene glycol alginate mixture Ethanol aqueous solution;

[0032] (4) Preparation of composite particles by emulsification evaporation method: using a vacuum rotary evaporator at 45℃, 300r/min, pressure -0.1MPa under reduced pressure and rotary evaporation to remove ethanol and some water; then centrifuge at 2000rpm for 30min , In order to remove larger particles to obtain a zein-propylene glycol alginate composite particle dispersion, which is dried to obtain zein-propylene glycol alginate composite particles.

[0033] The concentration of the composite particles in the zein-propylene glycol alginate composite particle dispersion obtained by the above method is 1.5%, the three-phase contact angle of the particles is 85°, and the particle size is 200-300nm; the SEM image of the composite particles Specific as figure 1 Shown from figure 1 It can be seen that the particle size is uniform without agglomeration.

[0034] The resulting zein-propylene glycol alginate composite particles are used as stabilizers to prepare Pickering emulsion: the dispersion obtained by dispersing the resulting zein-propylene glycol alginate composite particles in water is used as the aqueous phase, or the step (4 ) The obtained zein-propylene glycol alginate composite particle dispersion is used as the water phase; the medium chain fatty acid glyceride is used as the oil phase, and the oil phase is added to the water phase, and the volume fraction of the oil phase is 40% (v/v) , Shear at 8000rpm for 3min to prepare Pickering emulsion. The oil droplet size of the obtained Pickering emulsion is 20-60μm. After heating at 70°C for 30min, the oil droplet size of the emulsion maintains 20-60μm. After being placed for 30 days, no delamination occurs, and it has good thermal stability and storage. stability.

[0035] Example 2

[0036] Prepare prolamin-polysaccharide composite particles according to the following steps:

[0037] (1) Add 0.3 g of zein to 50 mL of ethanol aqueous solution (the volume fraction of ethanol is 70%), and stir at room temperature until completely dispersed to obtain an ethanol aqueous solution of zein;

[0038] (2) Add 0.03 g of propylene glycol alginate (the mass ratio of zein to propylene glycol alginate is 10:1) into 50 mL of ethanol aqueous solution (the volume fraction of ethanol is 70%), and magnetically stir until it is completely dissolved to obtain Aqueous solution of propylene glycol alginate in ethanol;

[0039] (3) Add the ethanol aqueous solution of zein obtained in step (1) to the ethanol aqueous solution of propylene glycol alginate obtained in step (2) under stirring conditions, and stir for 3 hours to obtain zein-propylene glycol alginate mixture ethanol Aqueous solution

[0040] (4) Using emulsification evaporation method to prepare composite particles: use a vacuum rotary evaporator at 50℃, 400r/min, pressure -0.1MPa under reduced pressure to remove ethanol and some water; then centrifuge at 3000rpm for 20min The larger particles are removed to obtain a zein-propylene glycol alginate composite particle dispersion, which is dried to obtain zein-propylene glycol alginate composite particles.

[0041] The concentration of the composite particles in the zein-propylene glycol alginate dispersion obtained by the above method is 1.0%, the three-phase contact angle of the composite particles is 90°, and the particle size is 200-400nm; the three-phase connection of the composite particles Tentacles figure 2 Shown.

[0042] The resulting zein-propylene glycol alginate composite particles are used as stabilizers to prepare Pickering emulsion: the dispersion obtained by dispersing the resulting zein-propylene glycol alginate composite particles in water is used as the aqueous phase, or the step (4 ) The obtained zein-propylene glycol alginate composite particle dispersion is used as the water phase; the medium chain fatty acid glyceride is used as the oil phase, and the oil phase is added to the water phase, and the volume fraction of the oil phase is 50% (v/v) , Shear at 10000rpm for 3min to prepare Pickering emulsion. The oil droplet size of the obtained Pickering emulsion was 20-30μm. After heating at 80°C for 30min, no layering or oil separation occurred in the emulsion, and the oil droplet size maintained 20-30μm; after 30 days, no layering occurred in the emulsion. It has good thermal stability and storage stability; the CLSM picture of the obtained Pickering emulsion is as follows image 3 Shown.

[0043] Example 3

[0044] Prepare prolamin-polysaccharide composite particles according to the following steps:

[0045] (1) Add 0.5 g of zein to 50 mL of ethanol aqueous solution (the volume fraction of ethanol is 75%), and stir at room temperature until it is completely dispersed to obtain an ethanol aqueous solution of zein;

[0046] (2) 0.1g of propylene glycol alginate (the mass ratio of zein to propylene glycol alginate is 5:1) is added to 50 mL of ethanol aqueous solution (the volume fraction of ethanol is 75%), and magnetically stirred until completely dissolved to obtain Aqueous solution of propylene glycol alginate in ethanol;

[0047] (3) Add the ethanol aqueous solution of zein obtained in step (1) to the ethanol aqueous solution of propylene glycol alginate obtained in step (2) under stirring conditions, and stir for 3.5 hours to obtain zein-propylene glycol alginate. Mixture ethanol aqueous solution;

[0048] (4) Using emulsification evaporation method to prepare composite particles: use a vacuum rotary evaporator at 55°C, 600r/min, and pressure -0.1MPa to remove ethanol and part of water; then centrifuge at 3500rpm for 25min The larger particles are removed to obtain the zein-propylene glycol alginate composite particle dispersion, which is dried to obtain the zein-propylene glycol alginate composite particles.

[0049] The concentration of the composite particles in the zein-propylene glycol alginate dispersion obtained by the above method is 1.5%, the three-phase contact angle of the particles is 93°, and the particle size is 300-400 nm.

[0050] The resulting zein-propylene glycol alginate composite particles were used as stabilizers to prepare Pickering emulsion, and the resulting zein-propylene glycol alginate composite particles were dispersed in water as the aqueous phase, or the step (4 ) The obtained zein-propylene glycol alginate composite particle dispersion is the water phase; the edible corn oil is used as the oil phase, and the oil phase is added to the water phase, the volume fraction of the oil phase is 60% (v/v), 12000 rpm Pickering emulsion was prepared by shearing for 4 min. The oil droplet particle size of the obtained Pickering emulsion was 20-40μm. After heating at 85°C for 30min, the emulsion did not appear delamination or oil separation, and the oil droplet size maintained 20-40μm; after 30 days, the emulsion did not appear delamination and Oil separation phenomenon has good thermal stability and storage stability.