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Compound degradation enzyme preparation of wheat vomitoxin and application method thereof

A technology of deoxynivalenol and enzyme preparation, which is applied in the field of compound degradative enzyme preparations of wheat deoxynivalenol, can solve the problems of lack of process parameters in degrading deoxynivalenol application cases, specific process parameters of deoxynivalenol degradation and data on deoxynivalenol degradation rate, etc. , to achieve good toxin degradation effect, large-scale application economy, and convenient use

Active Publication Date: 2019-07-19
合肥停弦渡生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The patent with the publication number CN108251386A "a vomitoxin-degrading enzyme and its gene, preparation method and application, and method for degrading vomitoxin" provides an amino acid sequence of a vomitoxin-degrading enzyme, but lacks specific methods for degrading vomitoxin. Application cases and specific process parameters; patent publication number CN108251386A "enzyme compound preparation, feed or additive and method for removing vomitoxin", the disclosed method is composed of two enzyme preparations, which has the effect of degrading vomitoxin , but the specific process parameters and data on the degradation rate of vomitoxin in the process of wheat flour milling are not found throughout the article

Method used

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  • Compound degradation enzyme preparation of wheat vomitoxin and application method thereof
  • Compound degradation enzyme preparation of wheat vomitoxin and application method thereof
  • Compound degradation enzyme preparation of wheat vomitoxin and application method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) if figure 1As shown, prepare 7.5kg of compound enzyme preparation; (wherein enzyme preparation A: enzyme preparation B: enzyme preparation C=1:10:1, enzyme preparation A includes: glucosyltransferase with activity not less than 30u / ml, activity not less than 30u / ml Acetylglucosamine transferase less than 30u / ml, alcohol dehydrogenase with activity not less than 200u / ml, the ratio of each component is 1:1:1. Enzyme preparation B includes: glucose oxidation with activity not less than 200u / ml Enzyme, horseradish peroxidase with activity not less than 10000u / ml, laccase with activity not less than 10000u / ml, the ratio of each component is 1:10:5. Enzyme preparation C includes: enzyme preparation C with activity not less than 1500u / ml Cellulase, pectinase with an activity of not less than 10000u / ml, and xylanase with an activity of not less than 8000u / ml, the distribution ratio of each component is 2:1:1, and the distribution ratios of the above components are all mass ...

Embodiment 2

[0042] (1) Prepare 10 kg of compound enzyme preparation (wherein enzyme preparation A: enzyme preparation B: enzyme preparation C=2:10:3, the component distribution ratio in enzyme preparation A is 2:2:1, and the component distribution ratio in enzyme preparation B The ratio of components in enzyme preparation C is 1:15:5, and the ratio of components in enzyme preparation C is 3:1:1, all of which are mass ratios; the enzyme preparations used are all from Hefei Tingxiandu Biotechnology Co., Ltd., and the processed wheat raw materials are all from Anhui Province Mengcheng County Hengrui Flour Co., Ltd.);

[0043] (2) Prepare 4.0 tons of water for the wheat watering process, adjust the pH of the water to 5.5 with acetic acid, control the water temperature at 15°C, and pour the above-mentioned compound enzyme preparation into the used water;

[0044] (3) 100 tons of mildewed wheat (with a water content of 12.0 to 14.0%, and a vomitoxin content of 2300ppb) is taken out of the wareh...

Embodiment 3

[0050] (1) Prepare 15 kg of compound enzyme preparation (wherein enzyme preparation A: enzyme preparation B: enzyme preparation C=1:10:1, the component distribution ratio in enzyme preparation A is 3:1:1, and the component distribution ratio in enzyme preparation B The ratio of components in enzyme preparation C is 1:10:5, and the ratio of components in enzyme preparation C is 2:2:1, all of which are mass ratios; the enzyme preparations used are all from Hefei Tingxiandu Biotechnology Co., Ltd., and the bran processed is all from Anhui Province Mengcheng County Hengrui Flour Co., Ltd.);

[0051] (2) Add 150 tons of vomitoxin-exceeding bran to above-mentioned compound enzyme preparation, mix;

[0052] (3) Prepare 150 tons of water, adjust the pH to 5.5 with acetic acid, and control the water temperature at 30° C., pour the above-mentioned bran mixed with the compound enzyme preparation (the vomitoxin content is 5000 ppb), and measure the vomitoxin content after keeping the temp...

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Abstract

The invention relates to a compound degradation enzyme preparation of wheat vomitoxin and an application method thereof, and belongs to the field of food safety. The compound degradation enzyme preparation comprises one or more of glycosyltransferase, acetyltransferase, alcohol dehydrogenase, glucose oxidase, horseradish peroxidase, laccase, cellulase, pectinase and xylanase. The degradation method is realized by the following two modes: 1) adding an enzyme preparation in the wheat milling process, and 2) adding an enzyme preparation in the re-processing process of flour and bran. The method is simple to operate in the wheat milling process, the preparation is convenient to use and small in dosage, and the method is low in cost, safe and environmentally friendly in the wheat milling process, and has remarkable effect of degrading the content of vomitoxin in main products of the wheat milling process such as flour and bran.

Description

technical field [0001] The invention relates to a compound degradation enzyme preparation of wheat vomitoxin and an application method thereof, belonging to the field of food safety. Background technique [0002] my country is a major producer of wheat, with an annual planting area and output of about 25% of the total grain. However, due to improper harvesting, storage or processing measures, wheat is easily contaminated with mycotoxins such as vomitoxin. Studies have shown that in the provinces where wheat is mainly grown in my country in recent years, the wheat has been polluted by vomitoxin to varying degrees. [0003] Deoxynivalenol (DON), also known as deoxynivalenol (DON), belongs to the trichothecene family compound, which is a secondary metabolite produced by fungi such as Fusarium graminearum and Fusarium yellow infecting grains , is the most important mycotoxin in scab wheat. DON is mainly distributed in the cortex of wheat grains. After the initial processing o...

Claims

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Application Information

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IPC IPC(8): C12N9/88C12N9/42C12N9/26C12N9/24C12N9/18C12N9/10C12N9/08C12N9/04C12N9/02A23L5/20A23K10/30A23K10/37
CPCA23K10/30A23K10/37A23L5/25C12N9/0006C12N9/0061C12N9/0065C12N9/1051C12N9/18C12N9/2408C12N9/2437C12N9/248C12N9/88C12Y101/01001C12Y101/03004C12Y110/03002C12Y111/01007C12Y301/01011C12Y302/01015C12Y402/02002Y02P60/87
Inventor 王林尹若春谷中华罗学才
Owner 合肥停弦渡生物科技有限公司
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