Application of Bacillus arborii in Preparation of Acidic Soil Improver
A technology of Bacillus aeruginosa, acid soil, applied in the field of microorganisms
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Embodiment 1
[0042] Acid-base tolerance of Bacillus arzii with deposit number CCTCC AB 207736:
[0043] Alkali resistance test:
[0044] Inoculate the Bacillus arborii seed liquid into LB medium with different initial pH according to the inoculum amount of 5%, cultivate it at 30°C and 200r / min, and take samples at regular intervals to measure OD 600 , and the measurement was not terminated until the late stage of stabilization. The initial pH gradient of the medium was set at 7, 8, 9, 10.
[0045] LB medium: peptone 10g, yeast extract powder 5g, NaCl 10g, distilled water 1000mL, pH 7.0, sterilized by high pressure steam at 121°C for 20min.
[0046] Test results such as figure 1 and figure 2 , the pH in the two groups of pH9 and pH10 has been in a downward trend from the beginning to 23h, it may be that the Bacillus arborii produces some acidic substance to neutralize the alkali in the medium so that the environment can achieve a suitable self-growth situation.
[0047] In the two group...
Embodiment 2
[0054] Phosphorus-solubilizing ability of Bacillus arborii with deposit number CCTCC AB 207736:
[0055] The seed solution was inoculated into the liquid phosphorus-dissolving medium according to the inoculation amount of 5%, and cultivated at 30°C and 200r / min, and then the available phosphorus (soluble phosphorus) content in the culture solution was measured by the molybdenum blue colorimetric method, so as to judge the soluble phosphorus content. The strength of phosphorus capacity.
[0056] Phosphorus-dissolving medium (PKO medium): glucose 10.0g, (NH 4 ) 2 SO 4 0.5g, NaCl 0.3g, KCl 0.3g, MgSO 4 ·7H 2 O 0.3g, FeSO 4 ·7H 2 O 0.03g, MnSO 4 ·7H 2 O 0.3g, insoluble phosphorus source, water 1000mL, pH 7.0, sterilized by high-pressure steam at 115°C for 30 minutes; insoluble phosphorus source and dosage: 0.5% tricalcium phosphate, 0.5% phosphate rock powder, 0.2% egg yolk lecithin (phosphoric acid Tricalcium 0.5% indicates that 0.5 g of tricalcium phosphate is added to...
Embodiment 3
[0063] Inhibitory effect of Bacillus arzii with preservation number CCTCC AB 207736 on plant pathogenic bacteria:
[0064] The inhibitory ability of Bacillus arzii to pathogenic bacteria was determined by zone of inhibition method. The activated Bacillus arzii seed solution was appropriately diluted and spread on a PDA plate, and the sterile water was used as a control group. Holes were punched on the PDA plate covered with pathogenic fungi, and the bacterial blocks were picked upside down and inoculated on the PDA plate coated with Bacillus arborii, so that the side with the fungus contacted the plate, and the plate was incubated at 30°C for 7 days. Then measure the fungal colony diameter, calculate the bacteriostatic rate, and then analyze the bacteriostatic effect.
[0065] The plant pathogenic bacteria selected in this experiment are banana fusarium wilt pathogen, potato scab pathogen, Verticillium verticillium, Fusarium oxysporum (2 strains), Fusarium solani (2 strains),...
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