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A method for extracting flavonoids with supercritical carbon dioxide

A technology for extracting flavonoids with carbon dioxide, which is applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, plant raw materials, etc., can solve the problems of unimproved extraction rate, unfavorable extraction, low extraction rate, etc., so as to improve the extraction rate of flavonoids, The effect of short time consumption and high extraction rate

Active Publication Date: 2021-10-15
赵春海
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, the extraction of flavonoids in Suaeda salsa is mainly based on ethanol extraction, water extraction, ultrasonic assisted extraction and microwave assisted extraction, wherein the water extraction and ethanol extraction are time-consuming ( time-consuming all in more than 3h), low extraction rate; though ultrasonic-assisted extraction and microwave-assisted extraction have solved the time-consuming problems of water extraction and ethanol extraction, the extraction rate has not been improved yet. Among the above four extraction methods, the extraction rate is about 6%, which is unfavorable for the extraction of flavonoids in Suaeda salsa

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Raw material handling

[0027] Take fresh Suaeda salsa, and dry it in a blast drying oven at 55°C; crush the dried Suaeda salsa with a pulverizer and pass through a 60-mesh sieve to obtain Suaeda salsa powder, which is set aside;

[0028] (2) Extraction of flavonoids

[0029] Take by weighing the Suaeda salsa powder 1.46kg of step (1), add compound enzyme solid 0.219kg and sodium dodecylbenzenesulfonate solid 29.2g, mix, obtain mixture; Wherein, compound enzyme solid comprises cellulase and alkaline The mass ratio of protease, cellulase and alkaline protease is 1:1;

[0030] Put the mixture into the extraction kettle for extraction, with CO 2 The fluid is the extractant, 85% ethanol is used as the entrainer, and the flow rate is 22mL / min; the extraction temperature is controlled at 45°C, the extraction pressure is 35Mpa, and the extraction time is 105min to obtain the flavonoid extract.

[0031] The flavonoid content in the flavonoid extract was detected, and the...

Embodiment 2

[0033] (1) Raw material handling

[0034] Take fresh Suaeda salsa, and dry it in a blast drying oven at 50°C; crush the dried Suaeda salsa with a pulverizer and pass through a 50-mesh sieve to obtain Suaeda salsa powder, which is set aside;

[0035] (2) Extraction of flavonoids

[0036] Take by weighing the Suaeda salsa powder 1.17kg of step (1), add composite enzyme solid 0.117kg and sodium dodecylbenzenesulfonate solid 11.7g, mix, obtain mixture; Wherein, described composite enzyme solid comprises cellulase and Alkaline protease, the mass ratio of cellulase and alkaline protease is 1:0.8;

[0037] Put the mixture into the extraction kettle for extraction, with CO 2 The fluid is the extractant, 95% ethanol is used as the entrainer, and the flow rate is 15mL / min; the extraction temperature is controlled at 40°C, the extraction pressure is 30Mpa, and the extraction time is 90min to obtain the flavonoid extract.

[0038] The flavonoid content in the flavonoid extract was dete...

Embodiment 3

[0040] (1) Raw material handling

[0041] Take fresh Suaeda salsa, and dry it in a blast drying oven at 60°C; crush the dried Suaeda salsa with a pulverizer and pass through a 70-mesh sieve to obtain Suaeda salsa powder, which is set aside;

[0042] (2) Extraction of flavonoids

[0043] Take by weighing the Suaeda salsa powder 1.31kg of step (1), add composite enzyme solid 0.262kg and sodium dodecylbenzenesulfonate solid 39.3g, mix, obtain mixture; Wherein, described composite enzyme solid comprises cellulase and Alkaline protease, the mass ratio of cellulase and alkaline protease is 1:1.2;

[0044] Put the mixture into the extraction kettle for extraction, with CO 2 The fluid is the extractant, 75% ethanol is used as the entrainer, and the flow rate is 30mL / min; the extraction temperature is controlled at 50°C, the extraction pressure is 40Mpa, and the extraction time is 120min to obtain the flavonoid extract.

[0045] The flavonoid content in the flavonoid extract was det...

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Abstract

The invention provides a method for extracting flavonoids by supercritical carbon dioxide, comprising: raw material processing: drying fresh Suaeda salsa at 50-60°C in a blast drying oven; pulverizing the dried Suaeda salsa with a pulverizer and Sieve to obtain Suaeda salsa powder; flavonoid extraction: take Suaeda salsa powder, add compound enzyme solid and sodium dodecylbenzenesulfonate solid, mix well to obtain a mixture; Suaeda salsa powder, compound enzyme solid and dodecylbenzene The mass ratio of sodium sulfonate solid is 10:1~2:0.1~0.3; the mixture is put into the extraction kettle for extraction, and the 2 Fluid is extractant, 95% ethanol is used as entrainer, and fluid flow, extraction temperature, extraction pressure and extraction time are controlled to obtain flavonoid extract. Using the method to extract the flavonoids effectively saves the use of solvents, shortens the extraction time, improves the extraction rate of the flavonoids, reduces environmental pollution, and is suitable for wide popularization and use.

Description

technical field [0001] The invention relates to the technical field of flavone extraction, in particular to a method for extracting flavone with supercritical carbon dioxide. Background technique [0002] Supercritical carbon dioxide extraction is based on the special dissolution of supercritical carbon dioxide on some special natural products, using the relationship between the dissolving capacity of supercritical carbon dioxide and its density, that is, the traditional separation using the influence of pressure and temperature on the dissolving capacity of supercritical carbon dioxide Compared with the extraction method, it has the advantages of high product purity, less damage to active ingredients and no heavy metal residue. [0003] Suaeda salsa is rich in a variety of nutrients, such as protein, fat, crude fiber, minerals, etc., which can be eaten as high-quality food materials; in addition, Suaeda salsa is also rich in flavonoids, which have anti-cancer, Anti-aging, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185
CPCA61K36/185A61K2236/19A61K2236/331A61K2236/37
Inventor 赵春海李文刘治刚孙国荣
Owner 赵春海
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