Bionic nano medicament and preparation method and application thereof
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A biomimetic nano-drug technology, applied in the field of its preparation, biomimetic nano-drugs, can solve the problem of low drug concentration and achieve the effect of convenient operation and mild conditions
Active Publication Date: 2019-08-09
HENAN UNIVERSITY
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[0003] The purpose of the embodiment of the present application is to provide a biomimetic nano drug, its preparation method and application, which aims to improve the problem of low drug concentration in the tumor of existing tumor drugs
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[0090] The present application also provides a preparation method of biomimetic nano-medicine, the preparation method of biomimetic nano-medicine comprises:
[0091] mixing the carrier, the first component and the second component, and then dialysis to remove the free first component and the second component to obtain the inner core;
[0092] Mix cancer cell membranes and cores, then extrude.
[0093] Further, mixing the carrier, the first component and the second component specifically includes:
[0094] Dissolving the carrier in tetrahydrofuran to obtain a first solution, dissolving the first component and the second component in anhydrous dimethyl sulfoxide to obtain a second solution, and then mixing the first solution and the second solution.
[0095] The carrier is dissolved in tetrahydrofuran first, the first component and the second component are dissolved in anhydrous dimethyl sulfoxide and then mixed, which is conducive to the uniform mixing of the three.
[0096] ...
Embodiment 1
[0117] The present embodiment provides a kind of anti-biological nano-medicine, which is mainly prepared by the following method:
[0118] Under the condition of nitrogen protection, the dried dextran (dextran, 10kDa) and 2-ethoxypropylene were reacted at room temperature in anhydrous dimethyl sulfoxide (DMSO) solvent for 3h, and a catalytic amount of p-toluene was added pyridinium sulfonate. After the reaction, the reaction was terminated with triethylamine, precipitated in deionized water with a pH of 8, and washed three times to prevent the degradation of the acetal bond. The final product m-dextran was obtained by freeze-drying the precipitate after centrifugation.
[0119] Extraction of cancer cell membrane (CM): After culturing glioma cells U87 MG cells in a cell culture dish, suck off the medium, wash with PBS, add 5mL of EDTA (2mM) to digest the cells, collect the cells, and use Wash once with PBS, then extract the cell membrane with a membrane protein extraction kit,...
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Abstract
The invention relates to the field of medicine, in particular to a bionic nano medicament and a preparation method and application thereof. The nano medicament comprises an inner core and a shell withwhich the inner core is coated; the inner core comprises a first component, a second component and a carrier the side chain of which contains sensitive bonds; the shell comprises a cancer cell membrane. The carrier of the sensitive bonds stays in a weak acid environment and is rapidly swelled, an anti-cancer medicament TMZ and the second component are efficiently released, and the cancer cell membrane serving as the shell is made into the nano medicament, wherein the cancer cell membrane has certain self-recognition and high tumor selectivity for targeting in-vivo homologous homing tumors, and the in-vivo circulation time of the nano medicament can be greatly prolonged. The cancer cell membrane serving as the shell and the carrier of the sensitive bonds efficiently release the anti-cancermedicament TMZ and the second component, and finally tumor cells are killed, so that the purpose of targeted synergistic treatment of human brain gliomas is achieved.
Description
technical field [0001] The application relates to the field of medicine, in particular, to a biomimetic nano drug, its preparation method and application. Background technique [0002] Malignant glioma is one of the most aggressive brain tumors. Surgery is used to resect the tumor volume, but the infiltrating tumor cells within the normal brain parenchyma cannot be completely removed, and the remaining tumor cells are protected by the blood-brain barrier (BBB) or blood-brain tumor barrier (BBTB), which hinders the delivery of chemotherapy drugs , so prone to tumor recurrence. Although nanoparticle-based drug delivery systems (DDS) have shown enhanced tumor-targeting capabilities, these DDSs cannot reach the full therapeutic potential of postoperative glioma treatment due to low intratumoral drug concentrations. Contents of the invention [0003] The purpose of the embodiments of the present application is to provide a biomimetic nano drug, its preparation method and ap...
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