Application of octacosanol in preparing drugs for preventing liver injury
A technology of octacosanol and liver damage, which is applied in the field of medicine and can solve the problems of limited drugs or methods for liver damage treatment
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[0027] The present invention has no special limitation on the source of the octacosanol, preferably octacosanol extracted from animal and plant waxes; the sources of the animal and plant waxes are preferably bran wax, sucrose wax, beeswax and One or several kinds of insect wax. The present invention has no special limitation on the preparation method of the octacosanol, and preferably includes the following steps in the embodiment of the present invention:
[0028] After mixing absolute ethanol, aviation kerosene, sodium hydroxide and animal and plant waxes, heating and reflux extraction is carried out to obtain an extract; after filtering the extract, cooling the supernatant and suction filtering to obtain a filter cake; Precipitating and drying the filter cake to obtain a crude product of octacosanol; performing rectification on the crude product of octacosanol to collect octacosanol.
[0029] In the present invention, the mass ratio of the volume of the absolute ethanol an...
Embodiment 1
[0037] Preparation of Octacosanol: Put 40g of beeswax in a 500mL three-neck flask, add 120mL of absolute ethanol, 280mL of aviation kerosene, add 4.8g of sodium hydroxide, heat to boiling in a water bath, stir and reflux for 4.5 hours, then transfer the reaction solution to a separatory funnel, washed with hot water several times, removed the lower aqueous phase, washed until the pH value was equal to 7, poured the supernatant into a beaker to cool overnight, and vacuum filtered to obtain a filter cake, which was desolvated and dried , to obtain the crude product of octacosanol; put the obtained crude product of octacosanol into a rectification kettle, heat it, and when the temperature of the kettle reaches 240°C, turn on the vacuum pump, keep the vacuum degree at 20-50Pa, and fully reflux for 30min Finally, the reflux ratio is controlled to be 1:10; according to the change of the tower top temperature, the main distillate at 210-235°C is collected, and detected by gas chromato...
Embodiment 2
[0039] (1) Take the octacosanol prepared in Example 1, add 5% DMSO (V / V) and 0.1% Tween 80 (V / V), and prepare 2000ug / mL octacosanol with sterilized double distilled water Alcohol suspension, heat and dissolve the octacosanol suspension in boiling water for 30 minutes in the dark, and then place the octacosanol solution in an ultrasonic instrument equipped with hot water at 90°C for 30 minutes. The alcohol solution becomes clear and reaches the sterilization effect; the prepared 2000ug / mL octacosanol solution is prepared with sterilized double distilled water to prepare 1000ug containing 5% DMSO (V / V) and 0.1% Tween 80 (V / V) / mL, 500ug / mL, 0ug / mL octacosanol solution, prepared with RPMI-1640 complete culture medium containing 200ug / mL, 100ug / mL, 50ug / mL, 0ug / mL octacosanol working concentration, where In the octacosanol working concentrations of 200ug / mL, 100ug / mL, 50ug / mL, and 0ug / mL, the final concentrations of DMSO and Tween 80 were 0.5% and 0.01% (V / V), respectively.
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