A kind of microalgae rapid screening device and microalgae rapid screening method
A screening device and fast technology, applied in the direction of microorganism-based methods, enzymology/microbiology devices, biochemical cleaning devices, etc., can solve the problems of inability to exchange air between algal strains and the outside world, low efficiency, etc., and save manpower and material resources and experimental materials, improve culture efficiency, and save space
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Embodiment 1
[0035] This embodiment provides a microalgae rapid screening device, such as Figure 1-3 As shown, it includes: culture tank 6, buoyancy device 2, orifice plate 3 and algae liquid culture tube 10; Below the orifice plate 3, at least one microalgae culture hole 8 is arranged on the orifice plate 3, the microalgae culture hole 8 is used to place the algae liquid culture tube 10, the algae liquid culture tube 10 is a cylindrical shape with an upper opening, and the algae liquid culture tube The bottom of the 10 is made of microporous glass.
[0036] Specifically, the diameter of the micropore is smaller than the diameter of the algae cells of the microalgae to be cultured, so that the algae cells can be trapped in the tube, and at the same time, the material exchange with the culture solution can be performed to the maximum extent.
[0037] Compared with the prior art, the microalgae rapid screening device provided in this embodiment can simultaneously complete the screening of ...
Embodiment 2
[0047] This embodiment provides a method for rapid screening of microalgae, using the screening device of the above-mentioned embodiment 1, specifically including the following steps:
[0048] Step 1: After adding a certain volume of culture solution to the culture tank, sterilize the entire rapid screening device in a pressure cooker and cool it down for later use;
[0049] Step 2: Use a rubber ferrule to fix the algae culture tube in the microalgae culture hole of the orifice plate, so that the algae culture tube below the rubber ferrule is immersed in the culture solution, and the part of the algae culture tube above the rubber ferrule is exposed in the air;
[0050] Step 3: After the algae colony is inserted into the algae liquid culture tube for cultivation, the target algae strain is detected and screened out by a microplate reader.
[0051] Specifically, before starting the cultivation in step 3, the gas required for cultivating the algal strains is passed into the cul...
Embodiment 3
[0053] This embodiment provides a method for rapid screening of microalgae. By measuring the growth of microalgae in different parts of the orifice plate, it is possible to evaluate whether the samples in the orifice plate of the device have parallelism. The algae species selected in this embodiment are Chlorella, using the screening device of the above-mentioned embodiment 1, specifically comprises the following steps:
[0054] Step 1: Draw the standard curve: plant the chlorella algae in the shake flask and pre-culture for 7 days, then centrifuge to remove the supernatant, freeze-dry the obtained algal cell pellet at -40°C for 24 hours to obtain dry algae powder ; Dilute the dry algae powder with distilled water to a certain concentration gradient, measure the absorbance at a wavelength of 570nm, and establish the dry weight and OD 570 Value of the unary linear regression equation, the linear regression equation is: y = 1105.1x + 0.0038 (R 2 =0.9983), where x represents dry...
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