Novel fermentation device and application thereof for immobilized yeast fermentation preparation of ethyl alcohol

A fermentation device and immobilized carrier technology, applied in the direction of supporting/immobilizing microorganisms, fermentation, biochemical cleaning devices, etc., can solve problems such as poor results, and achieve simple production, reduce the probability of bacterial contamination, and complete discharge Effect

Pending Publication Date: 2019-08-13
NANJING HIGH TECH UNIV BIOLOGICAL TECH RES INST CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a new type of fermentation device to prepare ethanol by fermenting immobilized yeast, so as to solve the problems of poor effect in the prior art

Method used

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  • Novel fermentation device and application thereof for immobilized yeast fermentation preparation of ethyl alcohol
  • Novel fermentation device and application thereof for immobilized yeast fermentation preparation of ethyl alcohol
  • Novel fermentation device and application thereof for immobilized yeast fermentation preparation of ethyl alcohol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Simulation of fermenter structure and mass transfer without circulation:

[0043] The 2,000-ton fermenter with a height-to-diameter ratio of 1.2 is divided into two layers. The immobilized balls connected in series are fixed at both ends of the upper and lower grids of each layer. The vertical distance is 4 cm, and the horizontal distance is 5 cm. The actual loading capacity is about 10,500. Bottom, middle layer propelling type blade 23 diameter 5.33m, and horizontal direction included angle 30 ° big head width 43cm, small head width 10cm, 3 blades. Top flat paddle, rotation diameter 5m, disk diameter 3.5m, blade length 1.5m, width 0.1m, perpendicular to the horizontal direction, 3 blades. Under the condition of rotating speed 12r / min, the mass transfer condition is as follows Figure 2a and Figure 2b As shown, the average flow velocity of the upper layer (A) is 0.16m / s, the average flow velocity of the lower layer (B) is 0.14m / s, and the overall average flow velocity ...

Embodiment 2

[0044] Embodiment 2 fermenter structure and mass transfer simulation with circulation

[0045] The circulating feed protrudes from the tank wall, with a design nozzle (such as Figure 3a Shown), other modes are with embodiment 1. Under the condition of rotating speed 12r / min, the mass transfer condition is as follows Figure 3b and 3c shown. The average flow velocity of the upper layer (A) is 0.17m / s, the average flow velocity of the lower layer (B) is 0.16m / s, and the overall average flow velocity is 0.165m / s, with better mass transfer effect.

Embodiment 3

[0046] Embodiment 3 is fermentation raw material with cassava liquefaction mash culture medium

[0047] The immobilized carrier is filled in the reactor in embodiment 2, with a circulation capacity of 900m 3 / h fix the seed solution for 48h (the seed medium is cassava mash, as described later). Until the bacterial cell adsorption concentration is 250 million / ml. Then prepare cassava liquefied mash: the ratio of solid to liquid is 1:2.3, add liquefying enzyme (16U / g cassava dry weight) to liquefy at 90°C for 1.5 hours, and discharge the immobilized seed waste liquid after cooling. Then add cassava liquefied mash (initial total sugar 226.0g / L, and add glucoamylase (180U / g cassava dry weight), liquid content 85%, carry out circular fermentation, wherein the temperature is 33 ° C, the pH of the fermentation liquid is 4.3, Velocity 900m 3 / h, the stirring speed is 12r / min, after the fermentation is finished, the fermentation liquid is drained, and the total residue of the feed l...

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Abstract

The invention discloses a novel fermentation device which comprises a fermentation tank body, a vertical stirring device, an immobilized carrier and a circulation pipeline for feeding and dischargingof the fermentation tank body; a plurality of feeding nozzles are annularly arranged at the side wall of the fermentation tank body for feeding of the fermentation tank body; a discharging outlet is formed in the bottom of the fermentation tank body for discharging of the fermentation tank body; a circulation pump is arranged between the discharging outlet and the feeding nozzles and connected through the circulation pipeline to form a circulation loop, materials inside the fermentation tank body enter the circulation pipeline through the discharging outlet, under the drive of the circulationpump, and the materials enter the fermentation tank body through the feeding nozzles for circulation fermentation; the circulation pipeline is further provided with a feeding pipe and a discharging pipe, and the feeding pipe and the discharging pipe are each provided with a one-way valve; a fermented culture medium is guided into the circulation pipeline through the feeding pipe and enters the fermentation tank body through the feeding nozzles; a fermented product passes through the circulation pipeline and is discharged through the discharging pipe. The invention further provides applicationof the fermentation device for immobilized yeast fermentation preparation of ethyl alcohol.

Description

technical field [0001] The invention belongs to the technical field of industrial biology, and in particular relates to a novel fermentation device and its application in preparing ethanol by fermenting immobilized yeast. Background technique [0002] With the shortage of natural resources and the increasingly prominent environmental problems, the status of biological fermentation technology is becoming more and more important, and it has also achieved rapid development in recent years. Due to its many advantages, immobilization technology has become an important research hotspot in biological fermentation. Although the research on immobilization technology has a long history, very few of them are actually applied to industrial production. The reason is that the immobilization medium has low mechanical strength, poor mass transfer effect, and difficult operation. Previously, we applied for the surface immobilized yeast fermentation technology (such as patent number ZL20121...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/02C12P7/10C12R1/865
CPCC12M25/00C12M27/02C12P7/10Y02E50/10
Inventor 应汉杰陈勇刘庆国赵南邹亚男汤德朋李飞剑甘诚
Owner NANJING HIGH TECH UNIV BIOLOGICAL TECH RES INST CO LTD
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