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Paracoccus denitrificans strain screening and application of paracoccus denitrificans in deodorization

A technology of denitrifying paracoccus and bacterial liquid, which is applied in the field of microorganisms, can solve the problems of reducing the degree of perception, and achieve the effect of fast desulfurization efficiency

Active Publication Date: 2019-08-13
XI'AN POLYTECHNIC UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The physical deodorization method relies on the transformation between solid, liquid and gas to eliminate foul odors, which only reduces the perception of smell by the sense of smell, but its chemical properties have not changed fundamentally

Method used

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  • Paracoccus denitrificans strain screening and application of paracoccus denitrificans in deodorization
  • Paracoccus denitrificans strain screening and application of paracoccus denitrificans in deodorization
  • Paracoccus denitrificans strain screening and application of paracoccus denitrificans in deodorization

Examples

Experimental program
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Effect test

Embodiment 1

[0042] Embodiment 1: strain screening

[0043] Collect 4L of activated sludge from the secondary sedimentation tank of a sewage treatment plant in Shuangliu, Sichuan, and add it to the reactor, and add 1L of activated sludge acclimation medium for aerated culture. According to the designed concentration gradient from low concentration to high concentration, each Concentration treatment of 5 days, 5 days, 5 days, 7 days, 8 days as a cycle, from low concentration to high concentration acclimatization 30 days after the completion of acclimatization culture. Take a small amount of supernatant and enrich in liquid denitrification and desulfurization medium 1 for 2 days, repeat 4 to 5 times and perform gradient dilution, starting from 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 Draw 0.2mL at the dilution level and spread it on a plate of denitrification and desulfurization medium 1, culture at a constant temperature of 30°C, obtain multiple colonies after multiple separations, pic...

Embodiment 2

[0047] Embodiment 2: bacterial strain identification

[0048] The isolated and purified strains were inoculated in denitrification and desulfurization medium 1 according to the same inoculum size, and placed in shakers at different temperatures (5°C, 10°C, 24°C, 27°C, 30°C, 33°C, 36°C , 40°C, 45°C, 50°C) for 12 hours, by measuring the OD 600 Determine the strain growth temperature is 10 ~ 50 ℃, the optimum growth temperature is 30 ℃. Take different initial pH (3.0, 4.0, 5.0, 6.0, 6.5, 7.0, 7.5, 8.0, 9.0, 10.0, 11.0) at 30 ℃ for 24h, measure the OD 600 It was determined that the growth pH of the bacteria was 4.0-10.0, and the optimum growth pH was 6.5. Take different C / N (1, 5, 10, 15, 20, 25, 30) to measure OD at pH 6.5 and temperature 30°C 600 It was determined that the tolerance range of the strain was C / N 5-25, and the optimum C / N was 15.

[0049] The isolated and purified bacterial strain was observed under a scanning electron microscope (SEM), and the results were as ...

Embodiment 3

[0052] Example 3: Denitrification and desulfurization test using denitrification and desulfurization medium 1

[0053] Sterilize 100mL denitrification and desulfurization medium one solution in a sterilizing pot at 115°C for 30 minutes, then insert 5% inoculum into the denitrification paracoccus cultured for 24 hours, and plug the bottle mouth with an aerobic stopper. Shake in a shaker (30°C, 200r / min).

[0054] The control group was added with the same amount of sterile water as the inoculum, each experimental group was set up three times, and the concentration of ammonia nitrogen, nitrate nitrogen and nitrite nitrogen was measured every 2 hours; the concentration of sulfide and elemental sulfur was measured every 0.5 hours, and the results take the average.

[0055] Compared with the blank control group, the removal efficiency of ammonia nitrogen in the culture medium added with TS-1 strain was significantly improved, and the ammonia nitrogen degradation rate reached 95.35%...

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Abstract

The invention belongs to the technical field of microbes, and particularly relates to paracoccus denitrificans TS-1, and the preservation number is CGMCC NO.17605. The paracoccus denitrificans has a 16SrDNA sequence shown in SEQ ID No.1. The invention further discloses application of the paracoccus denitrificans TS-1 in deodorization and sewage treatment. The paracoccus denitrificans TS-1 not onlyhas higher heterotrophic nitrification and aerobic denitrification capacities, but also has a stronger sulfide removal capacity, and is a strain with three functions.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a strain screening of denitrifying paracoccus and its application in deodorization. Background technique [0002] Odor pollutants refer to all gaseous substances that stimulate the sense of smell, cause people's unpleasantness and damage the living environment. There are many kinds of substances, and the scope of influence is large, and their treatment has gradually attracted people's attention. There are a large number of deodorizing microorganisms in poultry manure and domestic waste, and a large amount of ammonia and hydrogen sulfide will be produced during the landfill and composting process, which not only causes serious harm to the human body, but also pollutes the environment and groundwater sources. [0003] The current conventional deodorization methods are: physical deodorization method, chemical deodorization method, biological deodorization method. The p...

Claims

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Application Information

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IPC IPC(8): C12N1/20B01D53/84B01D53/58B01D53/56B01D53/48C02F3/34C12R1/01C02F101/16C02F101/10
CPCB01D53/84C02F3/34C02F2101/101C02F2101/16C02F2101/163C12R2001/01C12N1/205Y02A50/20
Inventor 李海红闫志英佟欣宇宦臣臣姬高升许力山房俊楠
Owner XI'AN POLYTECHNIC UNIVERSITY
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