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A strain of Pediococcus pentosaceus and its application

A technology of pediococcus pentosaceae and spherical shape, which is applied in the direction of application, bacteria, microorganisms, etc., and can solve the problems of low product activity and loss of activity

Active Publication Date: 2022-06-03
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This effect causes a huge change in the molecular structure of mycotoxins. This process is irreversible in animals and humans, and the converted products have very low or no activity.

Method used

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  • A strain of Pediococcus pentosaceus and its application
  • A strain of Pediococcus pentosaceus and its application
  • A strain of Pediococcus pentosaceus and its application

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Experimental program
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Embodiment

[0031] Screening, identification and detoxification mechanism of high-efficiency detoxified ZEN probiotic strains

[0032] 1.1 Source of strains

[0033] The 15 strains of lactic acid bacteria preserved in the clinical laboratory of the College of Animal Husbandry and Veterinary Medicine, Shenyang Agricultural University are as follows: Enterococcus faecalis, Clostridium butyricum, Lactobacillus salivarius, Enterococcus faecium, Lactobacillus reuteri, Pediococcus pentosaceus, Lactobacillus plantarum, Streptococcus lactis, Lactobacillus rhamnosus, Pediococcus thermophilus, Lactobacillus acidophilus, Lactobacillus brevis, Enterococcus lactis, Bacillus subtilis, Lactobacillus casei.

[0034] 1.2 Preparation of main medium

[0035] Lactic acid bacteria medium (MRS): peptone 10g, beef extract 10g, yeast extract 5g, diammonium hydrogen citrate 2g, glucose 20g, Tween 80 1mL, sodium acetate 5g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.58g, sulfuric acid Manganese 0.25g...

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Abstract

The invention discloses a strain of Pediococcus pentosaceae, which is proved to be a quadruple-shaped spherical Gram-positive facultative anaerobic bacterium through identification, and the preservation number is CCTCC No: M2018363. The Pediococcus pentosaceae xy46 of the present invention has a detoxification rate of 60.4% for live zearalenone (ZEN) bacteria with a concentration of 4 μg / mL; while the detoxification rate is as high as 94.4% under inactivation conditions. It has a significant protective effect on the damage to the reproductive system, reproductive organs and germ cells of mice caused by zearalenone (ZEN) at a concentration of 40mg / kg and 70mg / kg. Therefore, it can be used as a feed additive to prevent the toxic effects of zearalenone on animals.

Description

technical field [0001] The invention relates to the field of strains, in particular to a strain of Pediococcus pentosaceus and its application. Background technique [0002] Current research suggests that the main toxic mechanism of zearalenone (ZEN) is to interfere with the estrogen signaling pathway, thereby affecting the overall function of the reproductive system. There are two main interference pathways: the chemical structure of ZEN is very similar to 17β-estradiol (17β-E2), and it can compete with estrogen receptors to interfere with estrogen; in addition, ZEN and steroids It also has a similar structure and can compete with it for the binding sites of 3α-hydroxysteroid dehydrogenase (3α-HSD) and 3β-hydroxysteroid dehydrogenase (3β-HSD), affecting the synthesis of reproductive hormones and interfering with the estrogen signaling pathway. effect, resulting in dysfunction of the reproductive system. [0003] At present, the elimination methods of mycotoxins are genera...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18C12R1/01
CPCA23K10/18C12R2001/01C12N1/205
Inventor 龙淼潘佳雯何剑斌李鹏杨淑华张燚王楠王明阳
Owner SHENYANG AGRI UNIV