Unlock instant, AI-driven research and patent intelligence for your innovation.

One produces 1α,25(oh) 2 vd 3 media and methods

A culture medium and seed technology, applied in the field of drug biotransformation, can solve the problem of low yield

Active Publication Date: 2021-05-04
CHENGDU UNIV
View PDF10 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, existing microbial transformations produce 1α,25(OH) 2 VD 3 method, 1α,25(OH) 2 VD 3 low output

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Get pseudonocardia (Pseudonocardia autotrophica) CGMCCNo.7935 slant, dig block from the slant and insert in the 250mL shaking flask that 25mL seed culture medium is housed, on shaker 28 ℃, 240rpm cultivates 3 days, obtains seed liquid; Press 10 % Inoculum Amount Inoculate the seed solution into a 250mL shake flask containing 25mL of biotransformation medium, culture it on a shaker at 28°C and 240rpm for 2 days, and take 25mg / mL of 25OHVD 3 Add 1mL of ethanol solution into the biotransformation solution, continue the transformation and culture for 5 days, and then stop the transformation; take 2mL of the transformation solution, add 8mL of methanol, shake for 10 minutes, and centrifuge to get the supernatant for HPLC detection. After determination, 1α,25(OH) in shake flask 2 VD 3 The output reaches 580mg / L.

[0056] The seed medium and biotransformation medium used consist of: peptone 0.5g / l00mL, dextrin 0.5g / l00mL, sodium dihydrogen phosphate 0.2g / l00mL, magnesium sul...

Embodiment 2

[0058] Get pseudonocardia (Pseudonocardia autotrophica) CGMCCNo.7935 slant, dig block from the slant and put in the 250mL shaking flask that 25mL seed culture medium is housed, on shaker 28 ℃, cultivate 3 days at 240rpm, obtain seed liquid, press 10% Inoculation amount Inoculate the seed solution into a 250mL shake flask containing 25mL of biotransformation medium, culture it on a shaker at 28°C and 240rpm for 2 days, and take 25OHVD with a concentration of 25mg / mL 3 Add 1mL of ethanol solution into the biotransformation solution, continue the transformation and culture for 5 days, then stop the transformation; take 2mL of the transformation solution, add 8mL of methanol, shake for 10 minutes, centrifuge to get the supernatant for HPLC detection. After determination, 1α,25(OH) in shake flask 2 VD 3 The output reaches 470mg / L.

[0059] The seed medium and biotransformation medium used consist of: peptone 0.2g / l00mL, dextrin 1.0g / l00mL, sodium dihydrogen phosphate 0.1g / l00mL, ...

Embodiment 3

[0061] Get pseudonocardia (Pseudonocardia autotrophica) CGMCCNo.7935 slant, dig block from the slant and put in the 250mL shaking flask that 25mL seed culture medium is housed, on shaker 28 ℃, cultivate 3 days at 240rpm, obtain seed liquid, press 10% Inoculation amount Inoculate the seed solution into a 250mL shake flask containing 25mL of biotransformation medium, culture it on a shaker at 28°C and 240rpm for 2 days, and take 25OHVD with a concentration of 25mg / mL 3 Add 1mL of ethanol solution into the biotransformation solution, continue the transformation and culture for 5 days, then stop the transformation; take 2mL of the transformation solution, add 8mL of methanol, shake for 10 minutes, centrifuge to get the supernatant for HPLC detection. After determination, 1α,25(OH) in shake flask 2 VD 3 The output reaches 504mg / L.

[0062] The composition of seed medium and biotransformation medium used therein: peptone 1.0g / l00mL, dextrin 0.8g / l00mL, sodium dihydrogen phosphate ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of drug biotransformation, in particular to a method for producing 1α,25(OH) 2 VD 3 Biotransformation media and methods. The biotransformation medium includes nitrogen source, carbon source, inorganic salt A, inorganic salt B, trace elements and chitosan; in parts by weight, the dosage of each component is: 0.2-5.0 parts of nitrogen source, 0.5-5 parts of carbon source 5.0 parts, 0.1-1.0 parts of inorganic salt A, 0.1-1.0 parts of inorganic salt B, 0.0004-0.0012 parts of trace elements, 0.05-0.4 parts of chitosan. The present invention optimizes the formulation of the biotransformation medium, and finds that the increase of chitosan can significantly improve the 1α,25(OH) 2 VD 3 output.

Description

technical field [0001] The invention relates to the technical field of drug biotransformation, in particular to a method for producing 1α,25(OH) 2 VD 3 Biotransformation media and methods. Background technique [0002] Osteoporosis (Osteoporosis, OP) is a systemic bone disease in which bone density and bone quality decrease due to various reasons, and bone microstructure is destroyed, resulting in increased bone fragility and prone to fracture. Osteoporosis is divided into primary and secondary two categories. Primary osteoporosis is further divided into three types: postmenopausal osteoporosis (type I), senile osteoporosis (type II) and idiopathic osteoporosis (including juvenile type). Pain can reduce the patient's quality of life, spinal deformation and fracture can cause disability, restrict the patient's activities, make them unable to take care of themselves, increase the incidence of lung infection and decubitus ulcer, not only increase the patient's quality of lif...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/18C12R1/01
CPCC12N1/20C12P7/18
Inventor 曾志刚刘亚洲吴倩
Owner CHENGDU UNIV