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Detection primer group for spirit drug related genes, kit, system and method

A primer set and kit technology, applied in biochemical equipment and methods, microbial measurement/testing, recombinant DNA technology, etc., can solve the problems of long detection cycle, inapplicability of multi-gene and multi-site, low detection throughput, etc.

Active Publication Date: 2019-08-23
BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
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  • Abstract
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  • Application Information

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Problems solved by technology

[0003] At present, the detection of genes related to psychotropic drugs mainly includes PCR-Sanger sequencing method and fluorescent quantitative PCR method. Although the traditional conventional PCR-Sanger sequencing method is recognized as the gold standard for detecting genotyping, the detection cycle is long and The detection throughput is low, so it is not suitable for the detection of multi-gene and multi-locus; although the fluorescent quantitative PCR method has the advantages of high sensitivity, accurate typing, simple and fast operation, etc., it is also unable to meet the clinical requirements for multi-gene data conveniently and quickly. Ten to hundreds of site detection requirements

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  • Detection primer group for spirit drug related genes, kit, system and method
  • Detection primer group for spirit drug related genes, kit, system and method
  • Detection primer group for spirit drug related genes, kit, system and method

Examples

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Embodiment 1

[0075] The preparation method of embodiment 1 DNA sample to be tested

[0076] Oral exfoliated cells collected with buccal swabs or collected fresh peripheral blood samples were extracted with Tiangen Buccal Swab Genomic DNA Extraction Kit (DP322) or Blood / Cell / Tissue Genomic DNA Extraction Kit (DP304), and used NP80-touch (IMPLEN, Germany) measures the concentration and purity of DNA, and then preserves the genomic DNA.

Embodiment 2

[0077] Example 2 Polymorphism detection of genes related to psychotropic drugs for non-diagnostic purposes

[0078] According to literature research, 62 gene polymorphism sites of 15 psychotropic drug-related genes were selected, as shown in the above Table 1 and Table 2, and the DNA sequence of each polymorphic site was searched by NCBI, and the embodiment of the present invention was designed and synthesized For the design of primers, online / offline databases or software such as Primer Quest, Primer Premier 5.0, and DNAMAN can be used to design primers and analyze dimers and stem-loop mismatches to ensure that each amplified fragment They will not interact with each other to produce non-specific products or dimers, and then, if necessary, dilute to the required concentration such as 10 μM. The nucleotide sequences and product sizes of the amplification primers of the embodiments of the present invention are shown in Table 3. The primer set provided in this example covers all...

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Abstract

The invention provides a polymorphism detection primer group for spirit drug related genes, a kit, a reaction system and a method. The polymorphism detection primer group for the spirit drug related genes comprises at least one of 20 primer pairs for separately amplifying 62 polymorphism loci of 15 spirit drug related genes, wherein nucleotide sequences of upstream and downstream primers of the 20primer pairs are separately shown in SEQ ID No. 1-40. When the polymorphism detection primer group for the spirit drug related genes is used for carrying out polymorphism detection on the spirit drugrelated genes, any one or more of the 62 polymorphism loci of the 15 spirit drug related genes can be subjected to polymorphism detection, and the detection time can be effectively shortened.

Description

technical field [0001] The invention relates to the technical field of gene detection, in particular to a polymorphism detection primer set, kit, reaction system and method for genes related to psychotropic drugs. Background technique [0002] At present, the clinical treatment methods and means of mental illness are still mainly based on drug treatment, and patients with mental illness generally need long-term or even life-long medication, that is, patients with mental illness are highly dependent and sticky to drugs. Therefore, while psychotropic drugs are used clinically to treat and save patients, the adverse reactions caused by psychotropic drugs cannot be ignored. Clinical practice shows that in different stages of drug metabolism, transport, receptors, etc., when different individuals receive the same drug treatment, there are obvious differences in its therapeutic effect, side effects and drug tolerance. difference. Gene polymorphism is the essential cause of indiv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/156C12Q2600/106
Inventor 智慧芳佟洪梅赵方圆倪君君
Owner BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD