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Primer for detecting chlamydia trachomatis, probe and kit

A Chlamydia trachomatis and kit technology, applied in the field of nucleic acid detection, can solve the problems of low detection efficiency, false negatives, low specificity, etc., and achieve the effects of high detection efficiency, wide detection range and good specificity

Pending Publication Date: 2019-08-23
SHENZHEN GOLDSITE DIAGNOSTICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, kits for detecting CT DNA based on real-time fluorescent PCR technology have been used in clinical detection at home and abroad, but some kits have different degrees of toxicity due to different sample processing methods, primer and probe design sites, and reaction reagent tolerance. False negative results in low detection efficiency and low specificity

Method used

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  • Primer for detecting chlamydia trachomatis, probe and kit
  • Primer for detecting chlamydia trachomatis, probe and kit
  • Primer for detecting chlamydia trachomatis, probe and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Embodiment 1 kit performance evaluation

[0062] Detection of Chlamydia trachomatis in 10 cases of unknown female cervical swab samples by the kit of the present invention and the kit of comparative example.

Embodiment 2

[0082] The accuracy test of embodiment 2 kit

[0083] Detect 8 parts of enterprise positive reference products and 8 parts of enterprise negative reference products with kit of the present invention, detection method is carried out according to the kit operation steps of the present invention that embodiment 1 provides, and the result is as follows Figure 5 shown.

[0084] Depend on Figure 5 It can be seen that the positive reference products of the 8 companies all formed S-shaped amplification curves, and all of them could be judged as positive; the negative reference products of the 8 companies showed a flat amplification curve without a Ct value, and all of them could be judged as negative. The coincidence rate of positive reference products and negative reference products of the enterprise is 100%. It shows that the kit of the present invention has high accuracy.

Embodiment 3

[0085] The specificity test of embodiment 3 kits

[0086]In order to detect the specificity of the kit of the present invention, use the kit of the present invention to detect Ureaplasma urealyticum, Neisseria gonorrhoeae, Mycoplasma pneumoniae, Mycoplasma genitalium, Human papillomavirus type 18, Human cytomegalovirus, Mycoplasma hominis, Herpes simplex virus type 2 , Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Shigella dysenteriae. The detection method is carried out according to the operation steps of the kit of the present invention provided in Example 1, and the results are as follows: Figure 6 shown.

[0087] Depend on Figure 6 It can be seen that the positive control result is positive, and the above 12 kinds of pathogenic microorganisms are all negative. It shows that the kit of the present invention can only specifically amplify Chlamydia trachomatis, but does not cross-react with nucleic acids of other bacteria and viruses in the vagina, no ...

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Abstract

The invention discloses a primer for detecting chlamydia trachomatis, a probe and a kit, and relates to the technical field of nucleic acid detection. The primer is used for fluorescent PCR detectionof nucleic acid of the chlamydia trachomatis and comprises a forward primer body and a reverse primer body, wherein the nucleotide sequence of the forward primer body is shown in SEQ ID No:1, and thenucleotide sequence of the reverse primer body is shown in SEQ ID No:2. The nucleotide sequence of the probe is shown in SEQ ID No:3. The kit comprises a nucleic acid extracting solution, a PCR solution, a DNA polymerase, a positive control and a negative control, wherein the PCR solution comprises a reaction buffer solution, dNTPs, MgCl2, the primer and the probe. The invention aims to provide the fluorescent PCR kit for detecting the nucleic acid of the chlamydia trachomatis, and the kit has the advantages of being high in specificity and high in sensitivity.

Description

technical field [0001] The invention relates to the technical field of nucleic acid detection, in particular to a primer, a probe and a kit for detecting chlamydia trachomatis. Background technique [0002] Chlamydia trachomatis (CT) is a Gram-negative pathogen. It is a prokaryotic microorganism with a unique developmental cycle and strict intracellular parasitism. It can cause urogenital tract infection and is one of the most common venereal pathogens at home and abroad. It has been found that there are 15 serotypes, and different serotypes can cause different diseases. Chlamydia strains of type A, B, Ba, and C can cause trachoma, types L1, L2, and L3 can cause lymphogranuloma venereum, and types D-K can cause nongonococcal urethritis. Pathogen infection in CT often lacks specific symptoms, and it is easy to form occult infection, which brings difficulties to clinical diagnosis. At the same time, Chlamydia is easily mixed with other microbial pathogens during transmission,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/686C12Q1/04C12N15/11C12R1/01
CPCC12Q1/689C12Q1/686C12Q2563/107
Inventor 卢菲婷陈明峰高虹郑筱雯余嘉陵
Owner SHENZHEN GOLDSITE DIAGNOSTICS
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