Method of measuring ferric sodium chlorophyllate

A technology of chlorophyll and sodium iron, applied in the field of analytical chemistry, can solve problems such as low penetration rate and expensive instruments

Inactive Publication Date: 2019-08-23
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the expensive equipment, the NMR method is not widely used

Method used

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  • Method of measuring ferric sodium chlorophyllate
  • Method of measuring ferric sodium chlorophyllate
  • Method of measuring ferric sodium chlorophyllate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] (I) Determine the maximum absorption wavelength for ultraviolet detection: purchase ferric chlorophyllin sodium salt reagent, configure 0.005% ferric chlorophyllin sodium salt solution, weigh 5 mg of ferric chlorophyllin sodium salt solid, dissolve it in 100mL ultrapure water, pass through 0.22 μm polyvinylidene fluoride (PVDF) filter membrane, the sodium ferric chlorophyllin solution is stored in a brown reagent bottle (sodium ferric chlorophyllin is a water-soluble substance). Scan at 240-900 nm with a step size of 1 nm under a Shimadzu ultraviolet spectrophotometer, and determine that the maximum absorption wavelength is 392 nm.

[0046] (II) Detection by liquid chromatography: the injection volume of the 0.005% sodium ferric chlorophyllin solution configured in step (I) is 10uL, the wavelength of the ultraviolet detector is 392nm, the temperature of the column oven is set to 30°C, and the chromatographic column is Poroshell 120EC-C18 4μm, flow rate 1.0mL / min, mobile...

Embodiment 2

[0050] (I) Determine the maximum absorption wavelength for ultraviolet detection: purchase ferric chlorophyllin sodium salt reagent, configure 0.01% ferric chlorophyllin sodium salt solution, weigh 10 mg of ferric chlorophyllin sodium salt solid, dissolve it in 100 mL ultrapure water, pass through 0.22 μm polyvinylidene fluoride (PVDF) filter membrane, save the sodium ferric chlorophyllin solution in a brown reagent bottle. Scanning at 220-900nm with a step size of 1nm was carried out on a Mapuda UV spectrophotometer, and the maximum absorption wavelength was determined to be 395nm.

[0051] (II) Detection by liquid chromatography: the injection volume of the 0.01% sodium ferric chlorophyllin solution configured in step (I) is 10uL, the wavelength of the ultraviolet detector is 395nm, the temperature of the column oven is set to 30°C, and the chromatographic column is Poroshell 120EC-C18 4μm, flow rate 1.0mL / min, mobile phase: A: methanol: water: acetic acid = 60: 30: 10, B: m...

Embodiment 3

[0055] (I) Sample pretreatment: After extraction, centrifugation and passing through a 0.22 μm polyvinylidene fluoride (PVDF) filter membrane, the ferric chlorophyllin sodium salt synthesized by natural silkworm excrement was stored in a brown reagent bottle at 4°C.

[0056] (II) Determining the maximum absorption wavelength for ultraviolet detection: take 1 mL of ferric chlorophyllin sodium salt solution and dilute it by 10 times. The Hach DR3900 spectrophotometer scans at 300-900 nm with a step size of 1 nm, and determines that the maximum absorption wavelength is 395 nm.

[0057] (Ⅲ) Detection by liquid chromatography: Measure 1 mL of sodium ferric chlorophyllin solution into a brown liquid phase bottle (volume 2 mL). When the injection volume is 10uL, the wavelength of the ultraviolet detector is 395nm, the temperature of the column oven is set to 30°C, the chromatographic column is Poroshell120EC-C18 4μm, the flow rate is 1.0mL / min, the mobile phase: A: methanol: water: a...

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Abstract

The invention belongs to the field of analytical chemistry, and discloses a method of measuring ferric sodium chlorophyllate. The method comprises following steps of (1) establishing a standard curveequation: for multiple ferric sodium chlorophyllate standard solutions with known different concentrations, determining maximum absorption wavelength, then obtaining liquid chromatograms correspondingto the standard solutions through a liquid chromatograph, then determining a peak position of the ferric sodium chlorophyllate through mass detection, and obtaining a standard curve equation of peakarea-ferric sodium chlorophyllate concentration; and (2) measuring concentration of a to-be-tested sample: measuring and obtaining a liquid chromatogram of the to-be-tested sample, obtaining peak areaof the ferric sodium chlorophyllate, calculating based on the standard curve equation so as to obtain corresponding ferric sodium chlorophyllate concentration, so as to realize measurement of the ferric sodium chlorophyllate concentration. The whole process of the measurement method is explored, and liquid chromatography and mass spectrum are combined, so as to solve the technical problem of quantitative measurement of ferric sodium chlorophyllate.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and more specifically relates to a method for determining ferric chlorophyllin sodium salt. Background technique [0002] Chlorophyll iron sodium salt is a mixture of derivatives of chlorophyll, which is dark green solid or crystal obtained from natural silkworm excrement through saponification, acidification, iron substitution, and salt formation. For the treatment of iron deficiency anemia and food additives. At present, the detection methods of sodium iron chlorophyll include spectrophotometry, fluorescence photometry, high performance liquid chromatography, nuclear magnetic resonance and other methods. In spectrophotometry and fluorescence photometry, because the mixed chlorophyll and other derivatives in iron chlorophyllin sodium salt have the same absorption peak in the same spectral region, which interferes with the accurate quantification of iron chlorophyllin sodium salt, the accurac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 侯慧杰李珍雷龙海胡敬平刘冰川杨家宽梁莎肖可可
Owner HUAZHONG UNIV OF SCI & TECH
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