Homogeneous immunoassay kit for rapidly detecting procalcitonin and preparation method thereof, and detection method and device
A technology of procalcitonin and immunological reagents, applied in the field of immunoassay, can solve the problems of short shelf life, complicated washing steps, environmental pollution of radioimmunoassay, etc., and achieve the effect of rapid detection
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[0101] In some embodiments of the present invention, the receptors are polymer particles filled with light-emitting compounds and lanthanides, and the preparation method of the first composition includes:
[0102] Step S1, the first antibody or its binding fragment is dialyzed with a first dialysis buffer to obtain the dialyzed first antibody or its binding fragment;
[0103] Step S2, after the receptor is washed with the cross-linking buffer, the washed receptor is resuspended with the cross-linking buffer to obtain the receptor solution;
[0104] Step S3, adding the dialyzed first antibody or its binding fragment to the receptor solution, mixing well, and combining to obtain a receptor bound to the first antibody or its binding fragment;
[0105] Step S4, after washing the receptor bound to the first antibody or its binding fragment with washing buffer, adding the first buffer solution to obtain the first composition.
[0106] In some embodiments of the present invention, t...
Example Embodiment
[0181] Example 1: Preparation of a homogeneous immunoassay kit for rapid detection of procalcitonin
[0182] 1. Preparation of Acceptor and Donor
[0183] The preparation method, composition structure and component content of the acceptor and donor used in the present invention can be found in Example 1 of Chinese Patent CN100429197C (this patent document is hereby incorporated by reference in its entirety).
[0184] 2. Preparation of the first composition (R1)
[0185] (1) Preprocessing
[0186] Take 0.2mg of PCT Ab1 to be treated and put it into a dialysis bag (molecular weight cut-off is 14KD), put the dialysis bag into a beaker, add 100 times the volume of 0.05M CB buffer at pH 9.6 to the beaker, and place it on a magnetic stirrer , Dialyze at 2-8°C, replace the dialysate at least once, and dialyze at least 4-5 hours each time to obtain PCT Ab1 after dialysis, suck it out and transfer it to a clean centrifuge tube, and sample to measure the protein concentration.
[018...
Example
[0209] Implementation 2: Preparation of a homogeneous immunoassay kit for rapid detection of procalcitonin
[0210] Preparation of the first composition (R1)
[0211] According to the description in the examples of patent PCT / US2010 / 025433, PCT Ab1 is linked to the receptor to form a receptor that binds to PCTAb1. Wherein, the structure of the receptor is soluble, it is in non-particulate form, and the concentration of the receptor (component a) bound to PCT Ab1 in the first composition (R1) is 200 μg / mL.
[0212] The preparation of the remaining components is the same as that of Example 1.
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