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Artificial feed for harmonia axyridis larva as well as preparation method and application thereof

A technology of lady beetle axyridis and artificial feed, which is applied in the field of insect artificial feed and large-scale breeding, can solve the problems of inconvenient feeding, affecting the artificial mass breeding and breeding of lady beetles, and the biological control work cannot be carried out normally. Obtainable, low price, good anti-corrosion effect

Active Publication Date: 2019-08-27
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing artificial feed for ladybug heterochromia, some are pasty feed, some are powdery feed, all are inconvenient to take food
In the process of raising Heterochromia indoors, due to the shortage of aphid sources and the limitation of seasons, the artificial mass breeding of Heterochromia was affected, resulting in the failure of the normal biological control work in the next year.

Method used

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  • Artificial feed for harmonia axyridis larva as well as preparation method and application thereof
  • Artificial feed for harmonia axyridis larva as well as preparation method and application thereof
  • Artificial feed for harmonia axyridis larva as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: (preparation 100g artificial feed)

[0028] Feed formula:

[0029]

[0030] Heterochromia larvae artificial feed preparation method steps are as follows:

[0031] (1) Pick fresh apple snail eggs, place them in a homogenizer, add 3 mL of sterilized water to homogenate, filter the homogenate with a 100-mesh filter, remove the egg shells, and put the filtrate in a beaker as component A.

[0032] (2) Pulverize the complex vitamin B and vitamin C and the roasted soybeans and the pupa of Chilo borer, and remove the large particles by filtering with a 100-mesh filter screen;

[0033] (3) Glucose, sucrose, vitamin B complex and vitamin C are put into a beaker, and distilled water is added to dissolve, as the B component;

[0034] (4) mix the weighed soybean powder, the rice stem borer pupa powder and the yeast powder as the C component;

[0035] (5) Heat and melt the agar with water, then add sorbic acid and honey, boil, cool, add cholesterol, and use it as ...

Embodiment 2

[0043] Embodiment 2: (preparation 100g artificial feed)

[0044] Feed formula:

[0045]

[0046]

[0047] Heterochromia larvae artificial feed preparation method steps are as follows:

[0048] (1) Pick fresh apple snail eggs, place them in a homogenizer, add 3 mL of sterilized water to homogenate, filter the homogenate with a 100-mesh filter, remove the egg shells, and put the filtrate in a beaker as component A.

[0049] (2) Pulverize the complex vitamin B and vitamin C and the roasted soybeans and the pupa of Chilo borer, and remove the large particles by filtering with a 100-mesh filter screen;

[0050] (3) Glucose, sucrose, vitamin B complex and vitamin C are put into a beaker, and distilled water is added to dissolve, as the B component;

[0051] (4) mix the weighed soybean powder, the rice stem borer pupa powder and the yeast powder as the C component;

[0052] (5) Heat and melt the agar with water, then add sorbic acid and honey, boil, cool, add cholesterol, an...

Embodiment 3

[0060] Embodiment 3 Contrastive test of artificial feed and natural prey (pea aphid) on raising the effect of Heterochromia

[0061] Artificial feed: adopt the feed described in embodiment 1,2

[0062] Pea aphid: The broad bean is used as the host plant, and it is continuously raised in the greenhouse of the Key Laboratory of Biometrics, Inspection and Quarantine of Jiangxi Province.

[0063] Test method: The test insect feeding method refers to the method described in Example 1, and continuously feeds for 3 generations, each generation is equipped with artificial feed and 2 treatments of aphids, each treatment is repeated 3 times, and each repetition is 100. The developmental period of larvae, survival rate, pupal weight, pupal stage, egg production and egg hatching rate were recorded.

[0064] Statistics:

[0065] EXCEL was used for preliminary analysis of data, and SPSS 18.0 software was used for analysis of variance and significance test for all data.

[0066] The speci...

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Abstract

The invention discloses artificial feed for harmonia axyridis larva as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) placing egg massof ampullaria gigas in a homogenizer, adding an appropriate amount of sterilized water for homogenization, filtering to remove egg shells to serve as a component A. (2) crushing multi-vitamin B and vitamin C, and filtering to remove large particles; (3) putting glucose, sucrose, the multi-vitamin B and vitamin C into a beaker, and adding distilled water for dissolving to serve as a component B; (4) uniformly mixing soy flour, rice stem borer pupa powder and yeast powder to serve as a component C; (5) heating agar for melting with water, then adding sorbic acid and honey, boiling, cooling, andadding cholesterol to serve as a component D for standby use; (6) mixing the component A, the component B and the component C together, stirring evenly, then adding the component D, and continuing tosufficiently stir uniformly. The preparation method disclosed by the invention has the characteristics of low price, economical and practical effects, simple preparation and the like, and is suitablefor large-scale, long-term and subculture feeding of standard test insects.

Description

technical field [0001] The invention relates to an insect artificial feed and a large-scale feeding method, in particular to a heterochromia artificial feed and its preparation method and application. Background technique [0002] Harmonia axyridis is a common predatory natural enemy. Harmonia axyridis belongs to Coleoptera (Coleoptera), Coccinellidae (Coccinellidae), and is widely distributed in various regions of China except Hong Kong (Liu Zhen et al., 2009), has the characteristics of strong adaptability, wide feeding habits, large food intake, strong fecundity, etc., and has a strong control effect on the eggs of aphids, whiteflies, scale insects, mites and Lepidoptera pests. Dominant natural enemies have received extensive attention in world biocontrol research and applications (Koch, 2003). At present, the application of Heterochromia in biocontrol is mainly limited by large-scale artificial breeding and factory production, which are manifested in the lack of artific...

Claims

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Application Information

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IPC IPC(8): A23K10/18A23K10/20A23K10/30A23K20/111A23K20/158A23K20/163A23K20/174A23K50/90A01K67/033
CPCA23K10/20A23K10/30A23K10/18A23K20/174A23K20/163A23K20/158A23K20/111A23K50/90A01K67/033
Inventor 刘光富王世贵俞晓平张蓬军申屠旭萍
Owner CHINA JILIANG UNIV
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