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Lactobacillus rhamnosus and application thereof

A technology of Lactobacillus rhamnosus and strains, applied in Lactobacillus rhamnosus and its application field, can solve the problem of prolonging the survival period of tissue culture seedlings of A. , Improve the survival rate, and the effect of high social application value

Active Publication Date: 2019-08-27
INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there have been no relevant reports in the industry on the development of Lactobacillus rhamnosus to inhibit specific pathogenic bacteria (Vibrio vulnificus), health food or general food, nor the development of Lactobacillus rhamnosus Relevant reports on prolonging the survival period of tissue-cultured seedlings of Clematis clematis in bagging

Method used

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  • Lactobacillus rhamnosus and application thereof
  • Lactobacillus rhamnosus and application thereof
  • Lactobacillus rhamnosus and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0017] Embodiment 1 Lactobacillus rhamnosus ( Lactobacillus rhamnosus ) Isolation and identification of B156

[0018] 1. Strain isolation

[0019] Take 0.1 mL culture solution from healthy human feces fermented prebiotics (xylo-oligosaccharides) in vitro, distribute it into MRS liquid medium, and incubate at constant temperature and anaerobic conditions at 37°C until colonies appear, pick them up with an inoculation needle Bacteria that grow well on the edge are inoculated on a new MRS plate medium. After the newly inoculated bacteria grow into colonies, the endophytic bacteria on the edge are picked and cultured, and purified repeatedly until a single colony is obtained, named It is strain B156.

[0020] The formula of MRS liquid medium is: tryptone 10.0 g / L, beef powder 5.0 g / L, yeast powder 4.0 g / L, glucose 20.0 g / L, Tween 80 1.0 mL / L, K 2 HPO 4 •3H 2 O 1.5 g / L, anhydrous sodium acetate 3.0 g / L, ammonium citrate 2.0 g / L, MgSO 4 •7H 2 O 0.2 g / L, MnSO 4 •H 2 O 0.04 ...

Embodiment 2

[0025] Embodiment 2 Lactobacillus rhamnosus ( Lactobacillus rhamnosus ) Utilization of sugars by B156

[0026] API50CHL kit (Biomerieux, France) was used to detect the utilization of sugars by the bacterial strain B156 isolated in Example 1, and the results showed that Lactobacillus rhamnosus B156 of the present invention can utilize L-rhamnose, L-arabinose, ribose , D-glucose, D-mannose, D-fructose, D-galactose, mannitol, sorbitol, N-acetylglucosamine, amygdalin, arbutin, aescin, salicin (salixol) , D-cellobiose, D-maltose, D-lactose, D-mebose, D-sucrose, trehalose, D-melezitose, D-gentiobiose and D-turanose.

Embodiment 3

[0027] Embodiment 3 Lactobacillus rhamnosus ( Lactobacillus rhamnosus ) Enzyme activity of B156

[0028] APIZYM kit (Biomerieux, France) was used to detect the enzyme activity of the isolated bacterial strain B156 in Example 1, and the results showed that: Lactobacillus rhamnosus B156 of the present invention can secrete leucine arylase, valine arylase, cysteine Amino acid arylaminolase, β-glucosidase, N-acetyl-glucosaminidase, alkaline phosphatase, acid phosphatase, naphthol-AS-BI-phosphohydrolase, β-galactosidase and α-glucose sidase.

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Abstract

The invention discloses a strain of Lactobacillus rhamnosus. The strain with the preservation name of Lactobacillus rhamnosus B156, was preserved in China General Microbiological Culture Collection Center on September 10, 2018, and the preservation number is CGMCC No.16453. The invention further discloses application of the Lactobacillus rhamnosus in preparing medicines inhibiting Candida albicansand Vibrio vulnificus, health care products or common food, and in preparing a preparation prolonging survival time of bagging planting of Anoectochilus roxburghii tissue culture seedlings. The Lactobacillus rhamnosus B156 provided by the invention and fermented yoghurt prepared by the strain can remarkably inhibit the growth of the Candida albicans; the strain can remarkably improve the survivalrate of zebra fish attacked by the Vibrio vulnificus, and remarkably prolong the survival time of the bagging planting of the Anoectochilus roxburghii tissue culture seedlings. Therefore, the Lactobacillus rhamnosus B156 provided by the invention has relatively high social application value.

Description

technical field [0001] The invention relates to the technical field of microbial strains and applications thereof, in particular to a strain of Lactobacillus rhamnosus and applications thereof. Background technique [0002] Candida albicans ( Candida albicans ) are important opportunistic fungi that cause infection in immunocompromised hosts. Despite the treatment of antifungal drugs, the current case fatality rate of fungal bacteremia is still high, especially the case fatality rate of candida bacteremia is as high as 40%-80%. The reproduction of Candida albicans can cause Candida infection, such as vaginitis, balanitis and so on. Vibrio vulnificus ( Vibrio vulnificus ), or marine vibrio, is a bacterium that inhabits the ocean. Vibrio vulnificus is widely distributed in seawater, and Vibrio vulnificus can be isolated from seafood such as oysters. The bacteria mainly cause infection through wound contact with seawater, and can also be infected through the mouth. Inf...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P31/10A61P31/04A23L33/135A01G24/20C12R1/225
CPCA61K35/747A61P31/10A61P31/04A23L33/135A01G24/20A23V2002/00C12R2001/225C12N1/205A23V2200/30A23V2400/175
Inventor 黄菊青徐庆贤林斌王琦林晨韬郑怡钱蕾官雪芳张臣心
Owner INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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