Pepsinogen immunochromatographic detection kit based on carbon quantum dots, and preparation method thereof

An immunochromatographic detection and pepsinogen technology, which is applied in biological testing, analytical materials, measuring devices, etc., can solve the problem of limited specific binding ability of targets, improvement of unfavorable sensing and detection sensitivity, and lack of recognition bases for carbon quantum dots. Problems such as agglomeration can be solved to achieve good fluorescence characteristics, increase yield, and accurate detection

Active Publication Date: 2019-09-06
北京柏海达科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

When using the hydrothermal method to prepare carbon quantum dots with graphene as raw material, the fluorescence intensity can be enhanced by arylation modification of carbon quantum dots, but this method takes a long time and consumes a lot of energy to prepare carbon quantum dots , so the advantages of rapid preparation of carbon quantum dots and low energy consumption by microwave method are highlighted
[0009] With the increasing demand for high-sensitivity and high-specificity detection of actual samples and complex biological samples, the application of carbon quantum dots in biochemical detection also faces some pr

Method used

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  • Pepsinogen immunochromatographic detection kit based on carbon quantum dots, and preparation method thereof
  • Pepsinogen immunochromatographic detection kit based on carbon quantum dots, and preparation method thereof
  • Pepsinogen immunochromatographic detection kit based on carbon quantum dots, and preparation method thereof

Examples

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Embodiment 1

[0053] A preparation method of a pepsinogen immunochromatographic detection kit based on carbon quantum dots comprises the following steps:

[0054] S1: Preparation of carbon quantum dots:

[0055] a: Weigh 4g of citric acid in 30g of ultrapure water, stir at room temperature until the citric acid is completely dissolved;

[0056] b: Weigh 5g of ethylenediamine, add it to the solution prepared in step a, stir with a glass rod for 15-20min at room temperature, the solution is uniform, then add 35g of ultrapure water, and stir at room temperature for 30min at a speed of 400rpm, Store at 4°C for later use;

[0057] c: Take the solution prepared in step b in a microwave reactor, weigh 2.5g of ethyl 4-aminobenzoate in the microwave reactor, and react at a power of 500W and 300°C for 10min to obtain carbon quantum dots The product, the obtained carbon quantum dots are passivated by ethylenediamine and modified by arylation of ethyl 4-aminobenzoate, and become N-doped under the act...

Embodiment 2

[0072] The difference between this embodiment and Embodiment 1 is that in the preparation process of carbon quantum dots, the parameters of the amount of each raw material and the preparation conditions are different, and the specific steps are as follows:

[0073] Preparation of carbon quantum dots:

[0074] a: Take 5g of citric acid in 50g of ultrapure water, stir at room temperature until the citric acid is completely dissolved;

[0075] b: Weigh 6g of ethylenediamine, add it to the solution prepared in step a, stir evenly, add 65g of ultrapure water, stir at 400rpm for 30min, store at 4°C for later use;

[0076] c: Take the solution prepared in step b in a microwave reactor, and react 3g of ethyl 4-aminobenzoate in a microwave reactor at a power of 600W and 250°C for 10min to obtain a carbon quantum dot product, The obtained carbon quantum dots are passivated by ethylenediamine and modified by arylation of ethyl 4-aminobenzoate, and at the same time become N-doped fluores...

Embodiment 3

[0080] The difference between this example and example 1 is that the mercapto-containing organic compound added to the solution prepared in step f in step g is thioglycolic acid, and other steps are the same as in example 1.

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Abstract

The invention discloses a pepsinogen immunochromatographic detection kit based on carbon quantum dots, and a preparation method thereof. The pepsinogen immunochromatographic detection kit comprises aplastic plate and an adsorption layer fixed on the plastic plate, wherein the adsorption layer is composed of a sample pad, a glass cellulose film, a nitrocellulose film and a water absorbent pad which are in lap joint with each other from the test end in sequence; the glass cellulose film is coated with carbon quantum dot-labeled pepsinogen I and pepsinogen II; the nitrocellulose film is providedwith a detection line 1, a detection line 2 and a control line, the detection line 1 is coated with pepsinogen I secondary antibodies, the detection line 2 is coated with pepsinogen II secondary antibodies, and the control line is coated with goat-anti-mouse IgG; and the carbon quantum dots are respectively modified by means of ethylenediamine and N-hydroxysuccinimide. The pepsinogen immunochromatographic detection kit of the invention can be used for combined detection of the pepsinogen I and the pepsinogen II, and has the advantages of detecting pepsinogen in serum efficiently in a low-toxicity manner.

Description

technical field [0001] The invention relates to the field of immunochromatographic detection kits of carbon quantum dots, more specifically, it relates to an immunochromatographic detection kit of pepsinogen based on carbon quantum dots and a preparation method thereof. Background technique [0002] Pepsinogen (Pepsinogen, pepsinogen) is synthesized by the chief cell of the oxyntic gland, and is a single-chain polypeptide with a molecular mass of 42KDa, which is converted into a glycoprotein by the action of hydrochloric acid (HCL) or active pepsin in the gastric cavity. Pepsin is the inactive precursor of pepsin in gastric juice, which decomposes protein into fat, peptone and a small amount of polypeptide. Human pepsinogen can be divided into 7 groups according to its electrophoretic mobility, and divided into 2 subgroups according to its biochemical properties and immunogenicity. Components 1-5 have the same immunogenicity and are called pepsinogen I ( Pepsinogen I), secr...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/58G01N33/574G01N33/558G01N33/533G01N33/531G01N21/64
CPCG01N33/6893G01N33/57446G01N33/558G01N33/582G01N33/531G01N33/533G01N21/6428G01N2021/6439
Inventor 王瑛琦李想黄洋鞠文东
Owner 北京柏海达科技有限公司
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