Pepsinogen immunochromatographic detection kit based on carbon quantum dots, and preparation method thereof

Abstract

The invention discloses a pepsinogen immunochromatographic detection kit based on carbon quantum dots, and a preparation method thereof. The pepsinogen immunochromatographic detection kit comprises aplastic plate and an adsorption layer fixed on the plastic plate, wherein the adsorption layer is composed of a sample pad, a glass cellulose film, a nitrocellulose film and a water absorbent pad which are in lap joint with each other from the test end in sequence; the glass cellulose film is coated with carbon quantum dot-labeled pepsinogen I and pepsinogen II; the nitrocellulose film is providedwith a detection line 1, a detection line 2 and a control line, the detection line 1 is coated with pepsinogen I secondary antibodies, the detection line 2 is coated with pepsinogen II secondary antibodies, and the control line is coated with goat-anti-mouse IgG; and the carbon quantum dots are respectively modified by means of ethylenediamine and N-hydroxysuccinimide. The pepsinogen immunochromatographic detection kit of the invention can be used for combined detection of the pepsinogen I and the pepsinogen II, and has the advantages of detecting pepsinogen in serum efficiently in a low-toxicity manner.

Description

technical field [0001] The invention relates to the field of immunochromatographic detection kits of carbon quantum dots, more specifically, it relates to an immunochromatographic detection kit of pepsinogen based on carbon quantum dots and a preparation method thereof. Background technique [0002] Pepsinogen (Pepsinogen, pepsinogen) is synthesized by the chief cell of the oxyntic gland, and is a single-chain polypeptide with a molecular mass of 42KDa, which is converted into a glycoprotein by the action of hydrochloric acid (HCL) or active pepsin in the gastric cavity. Pepsin is the inactive precursor of pepsin in gastric juice, which decomposes protein into fat, peptone and a small amount of polypeptide. Human pepsinogen can be divided into 7 groups according to its electrophoretic mobility, and divided into 2 subgroups according to its biochemical properties and immunogenicity. Components 1-5 have the same immunogenicity and are called pepsinogen I ( Pepsinogen I), secr...

AI Technical Summary

Problems solved by technology

When using the hydrothermal method to prepare carbon quantum dots with graphene as raw material, the fluorescence intensity can be enhanced by arylation modification of carbon quantum dots, but this method takes a long time and consumes a lot of energy to prepare carbon quantum dots , so the advantages of rapid preparation of carbon quantum dots and low energy consumption by microwave method are highlighted

[0009] With the increasing demand for high-sensitivity and high-specificity detection of actual samples and complex biological samples, the application of carbon quantum dots in biochemical detection also faces some problems, such as: Compared with fluorescent dyes, the fluorescence of most carbon quantum dots The quantum yield is generally low, which is not conducive to the application of biological imaging and the improvement of the sensitivity of sensing and detection; the surface of some carbon quantum dots lacks effective recognition groups, resulting in limited specific binding ability with the target, and the selectivity needs to be improved; The interference caused by the concentration of the probe itself and environmental factors on the fluorescence intensity is inevitable, which limits the improvement of detection accuracy.

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