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A Heparin Sodium Wastewater Treatment Process for Recovering Protein

A waste water treatment and heparin sodium technology, which is applied in the field of protein recovery process, can solve the problems of difficult waste water treatment, high content, waste of protein, etc., and achieve the effects of reducing the amount of hanging on the wall, improving the recovery rate, and high extraction efficiency

Active Publication Date: 2021-12-07
GUANGYUAN HAIPENG BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is that at present, the waste water after heparin sodium extraction contains a large amount of protein, the waste water treatment is difficult, and a large amount of protein will be wasted. The problem that the waste water after extraction contains a large amount of protein

Method used

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  • A Heparin Sodium Wastewater Treatment Process for Recovering Protein

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A kind of heparin sodium wastewater treatment process of the present invention reclaims protein, comprises the following steps:

[0034] S1. Obtain heparin sodium wastewater, adjust the pH value of the wastewater to 7-8, and use a dynamic resin adsorption column to adsorb the wastewater to obtain a filtrate;

[0035] S2. Reduce the temperature of the filtrate to 10-20°C, add ethanol to the filtrate, and stir continuously until precipitation occurs, then slowly heat up to 30-40°C after precipitation, keep warm for 20-40 minutes, and filter to obtain the first clear liquid, crude protein;

[0036] S3. Adjust the pH of the first clear liquid to 8-10, add aluminum trichloride solution to the first clear liquid, add flocculant after stirring, react for 30-60min under the condition of stirring, then turn off the stirring and let stand for stratification ;

[0037] S4. Centrifuging the supernatant after standing and stratifying, the separated solid is crude protein, and coll...

Embodiment 2

[0044] Based on Example 1, such as figure 1 As shown, the dynamic resin adsorption column includes a vertically placed cylinder 1, a plurality of arc-shaped covers 2 are arranged inside the cylinder 1, the arc-shaped covers 2 are hollow hemispheres, and the arc-shaped covers 2 Curved upwards, each arc-shaped cover body 2 is provided with a diversion groove 3, the diversion groove 3 is a hollow hemisphere bent downward, and the arc-shaped cover body 2, diversion groove 3 and cylindrical tube 1 are all Cut and tightly connected, the inside of the cylinder 1 between the arc-shaped cover body 2 and the diversion groove 3 is filled with resin, and the resin is compacted between the arc-shaped cover body 2 and the diversion groove 3 to form a resin column, and the arc-shaped cover The side wall near the bottom of the body 2 is a hollow wall, and the bottom of the diversion groove 3 is provided with a connecting pipe 4 communicating with the interior of the diversion groove 3. The co...

Embodiment 3

[0047] Based on the above-mentioned embodiment, a horizontally placed liquid inlet pipe 5 is arranged above each arc-shaped cover body 2, the liquid inlet of the liquid inlet pipe 5 is located outside the cylinder 1, and the liquid outlet of the liquid inlet pipe 5 is located on the cylinder 1 Inside, the liquid outlet of the liquid inlet pipe 5 is bent downward to form a straight pipe section, and the liquid outlet of the liquid inlet pipe 5 is coaxial with the arc-shaped cover body 2 . The bottom of the cylinder 1 is provided with a waste liquid cylinder 6 communicating with the cylinder 1 , and the liquid inlet ends of all the liquid inlet pipes 5 are connected with the waste liquid cylinder 6 .

[0048] The closer the resin column is to the top of the cylinder, the easier it is to reach saturation. Once the resin column reaches saturation, it cannot continue to absorb heparin sodium in the waste water; Repeated adsorption. After the waste liquid passes through the cylinder...

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Abstract

The invention discloses a heparin sodium waste water treatment process for recovering protein, comprising the following steps: obtaining heparin sodium waste water, adopting a dynamic resin adsorption column to absorb the waste water to obtain a filtrate; adding ethanol to the filtrate, and filtering after precipitation to obtain the first One supernatant, crude protein; add aluminum trichloride solution and flocculant to the first supernatant, and then stand for stratification; centrifuge the first supernatant after standing and stratification, and the separated solid is crude Protein, collect the separated second clear liquid; add trichloroacetic acid to the second clear liquid, stir until the precipitate precipitates out, and centrifuge after standing to obtain the precipitate and the third clear liquid, the precipitate is crude protein; Separate the nanofiltration membrane to concentrate the third clear liquid, add sodium chloride solution to the concentrated liquid for salting out, filter to obtain crude protein; mix the crude protein to obtain mixed protein, and purify after drying to obtain high-quality protein. The invention has high recovery rate of protein.

Description

technical field [0001] The invention relates to a protein recovery process, in particular to a heparin sodium wastewater treatment process for protein recovery. Background technique [0002] Heparin sodium (Heparin sodium) can interfere with many links in the blood coagulation process, and has anticoagulant effects both in vivo and in vitro. Its mechanism of action is relatively complex, mainly by combining with antithrombin III (AT-III), and enhancing the inhibitory effect of the latter on activated coagulation factors II, IX, X, XI and XII, and its consequences involve preventing platelet aggregation and Destroy and hinder the formation of thromboplastin, prevent prothrombin from turning into thrombin, inhibit thrombin, thereby preventing fibrinogen from turning into fibrin, thereby exerting an anticoagulant effect. [0003] At present, the extraction method of heparin sodium usually adopts the resin adsorption method, that is, the resin absorbs the heparin sodium in the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F9/04C07K1/36C07K1/34C07K1/30C02F103/36
CPCC02F1/001C02F1/28C02F1/38C02F1/442C02F1/56C02F9/00C02F2103/36C02F2301/08C07K1/30C07K1/34C07K1/36
Inventor 李海生
Owner GUANGYUAN HAIPENG BIOLOGICAL TECH
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