SNP molecular marker which is positioned on No.7 porcine chromosome and related to area and thickness of eye muscles and application

A technology of eye muscle area and molecular markers, which is applied in the field of molecular biotechnology and molecular markers, can solve the problems of inability to detect genes with unknown functions, large confidence intervals for QTL positioning, and failure to meet the needs of applications, so as to increase core competitiveness, Rapid and accurate breeding, the effect of improving corporate profits

Active Publication Date: 2019-09-10
SOUTH CHINA AGRI UNIV
View PDF2 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previously, people used QTL mapping and candidate gene method to identify the main genes, but both methods have their own disadvantages. The QTL positioning confidence interval is relatively large and will be affected by d

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • SNP molecular marker which is positioned on No.7 porcine chromosome and related to area and thickness of eye muscles and application
  • SNP molecular marker which is positioned on No.7 porcine chromosome and related to area and thickness of eye muscles and application
  • SNP molecular marker which is positioned on No.7 porcine chromosome and related to area and thickness of eye muscles and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Experimental animals

[0036] The experimental pig group used in the present invention is 2153 purebred plus line Duroc of Wen's Food Group Co., Ltd. Breeding Pig Branch, which is the core group of Breeding Pig Branch.

[0037] In the resource group selected in this experiment, the Canadian Duroc breed pigs, the pigs have free access to food and water, and the entire feeding method and feeding conditions are always consistent, which is a conventional method.

[0038] (2) Sample collection

[0039] The above-mentioned piglet docked tail and ear tissues were collected and soaked in ethanol solution with a volume fraction of 75%, and stored in a -20°C refrigerator for later use.

[0040] (3) 50K SNP typing in the whole pig genome

[0041] The ear tissue or docked tail tissue collected from each individual of the 2153 Duroc breeding pigs selected from the above-mentioned resource groups, the whole genome DNA was extracted by the standard phenol-chloroform method, and ...

Embodiment 2

[0053] Example 2 Target DNA sequence amplification and sequencing

[0054] (1) Primer design

[0055] The DNA sequence of SEQ ID NO: 1 on pig chromosome 7 was downloaded from the Ensembl website (http: / / asia.ensembl.org / index.html). And use primer design software primer premier 6.0 to design primers. The DNA sequences of the designed primers are as follows:

[0056] P001-F: 5'-TTGTCACTGTTCAGCAACCCAC-3';

[0057] P002-R: 5'-CCCACAAGCCAAGCCAGA-3';

[0058] (2) PCR amplification

[0059] Add 1 μL of DNA template, 3.4 μL of double distilled water, 5 μL of 2×Tag PCR StanMix with Loading Dye, and 0.3 μL of primers P001-F and P002-R into a 10 μL reaction system. The PCR reaction conditions were as follows: 3 minutes of pre-denaturation at 95°C, 35 cycles of denaturation at 95°C for 30 s, annealing at 65°C for 30 s, extension at 72°C for 30 s, and finally 5 min at 72°C.

[0060] (3) DNA sequence determination

[0061] DNA sequence sequencing identification: carried out in Shenz...

Embodiment 3

[0064] Example 3 SNP site g.72A>G effect analysis of molecular markers

[0065] According to the analysis results of Table 1 and Table 2, the effect of this site on the eye muscle area and eye muscle thickness of pigs reached a very significant level (P2 , the average eye muscle thickness is 1.02mm larger 2 . Therefore, through molecular marker-assisted selection, eliminating the pigs with genotype AA in the population can significantly increase the eye muscle area and eye muscle thickness of the population, promote the breeding process of Duroc breeding pigs, increase the eye muscle size of pork, and improve the quality of pork. Lean meat rate, so as to produce high-quality pork, promote the increase of pork sales, and also drive the economic benefits of the pig industry to be greater.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of molecular biotechnology and molecular markers and particularly relates to an SNP molecular marker which is positioned on a No.7 porcine chromosome and related to the area and thickness of the eye muscles and application. An SNP locus of the SNP molecular marker positioned on the No.7 porcine chromosome and related to the area and thickness of the eyemuscles corresponds to a mutation (A>G) of the 30342161st site of the No.7 chromosome of an international porcine genome 11.1 version reference sequence. The invention also provides a primer pair foridentifying the molecular marker. Through the molecular marker and the primer pair, dominant alleles of SNP can be screened generation by generation, the frequency of the dominant alleles is increased, the area and thickness of the eye muscles of a pig are increased, the economic benefit of pork is finally increased, and the molecular marker and the application have very important significance inthe progress of genetic improvement of pigs.

Description

technical field [0001] The invention belongs to the technical field of molecular biotechnology and molecular markers, and specifically relates to a SNP molecular marker located on pig chromosome 7 and related to eye muscle area and eye muscle thickness and its application. Background technique [0002] my country is a big country in pig production and pork consumption in the world. Both the number of breeding and pork consumption ranks first in the world. The level of pork consumption occupies a dominant position in my country's overall meat consumption. With the growth of population, people's demand for livestock and poultry agricultural products is increasing, which requires us to speed up the cultivation of breeding pig breeds with more meat and better meat quality. If the appearance factors such as nutritional regulation and production environment are improved to improve meat quality, the effect is often unsatisfactory. In fact, genetic factors have a decisive effect on...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 杨杰吴珍芳彭珑珑庄站伟徐铮郑恩琴蔡更元
Owner SOUTH CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap