Baicalin liposome ointment and preparation method thereof

A technology of baicalin and baicalin, applied in the field of baicalin liposome ointment and preparation thereof

Active Publication Date: 2019-09-13
QUANZHOU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no literature report about baicalin liposome ointment

Method used

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  • Baicalin liposome ointment and preparation method thereof
  • Baicalin liposome ointment and preparation method thereof
  • Baicalin liposome ointment and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Preparation of Baicalin Liposomes

[0031] Weigh 18 mg of baicalin powder into a container, add 6 mL of pH 7.0 phosphate buffer solution, stir to dissolve completely, and obtain a 3 mg / mL baicalin solution. Separately weigh 200 mg of soybean lecithin, 2 mg of vitamin E, and 34 mg of cholesterol into a beaker, add 6 mL of ether and 12 mL of chloroform to mix and dissolve, stir well, and slowly pour into the prepared baicalin solution. Sonicate in a water bath for 8 minutes to obtain a yellow upper layer and a milky white lower layer solution. Rotate to a colloidal state at 40°C. Add 1.5mL Tween 80 and 5mL pH7.0 phosphate buffer solution, continue to rotate and mix until a pale yellow solution is formed. Baicalin liposomal suspension.

Embodiment 2

[0033] Selection of Baicalin Detection Wavelength

[0034] Take an appropriate amount of baicalin solution and the prepared blank liposomes, and dilute them in a 10 mL volumetric flask with normal saline, respectively. Full-wavelength scanning was performed using UV spectrophotometry, ranging from 200-400 nm. Depend on figure 1 It can be seen that the baicalin solution has a maximum absorption wavelength at 214nm and 276nm, and the blank liposome has a greater impact on it at 214nm, while the blank liposome does not interfere with it at 276nm, so it is advisable to select the detection wavelength at 276nm.

Embodiment 3

[0036] Determination of Encapsulation Efficiency of Baicalin Liposome

[0037] Take 1 mL of baicalin liposome suspension and put it into an ultrafiltration centrifuge tube, centrifuge in a refrigerated centrifuge for 30 min, and adjust the speed to 7000 r / min. Take all the filtrate at the bottom of the ultrafiltration centrifuge tube and dilute to constant volume, measure its absorbance, combine the standard curve to calculate the free baicalin concentration as c 2 . Take another 1mL baicalin liposome suspension of the same batch, dilute to the same multiple, measure its absorbance with UV, and convert the total concentration of baicalin into c 1 . The encapsulation rate calculation formula is:

[0038] Several batches of baicalin liposomes were prepared, and the encapsulation efficiency was (85.24±2.89)% measured under the same conditions, and the RSD was 3.39%. The specific results are shown in Table 1.

[0039] Table 1 baicalin liposome encapsulation efficiency assay ...

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PUM

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Abstract

The invention discloses a baicalin liposome ointment and a preparation method thereof. The ointment is prepared from baicalin liposome, azone and a matrix through grinding; and the components of the ointment include soya bean lecithin, cholesterol, baicalin, vitamin E, the azone and carboxymethylcellulose sodium. The ointment can promote the transdermal absorption of the baicalin; the disadvantages of poor dissolvability of the baicalin can be overcome; the bioavailability of the baicalin can be enhanced; and the ointment is slow in drug release speed and long in drug effect and has a certainslow release effect.

Description

technical field [0001] The invention belongs to the field of pharmaceutical preparations, and in particular relates to a baicalin liposome ointment and a preparation method thereof. Background technique [0002] Scutellaria baicalensis is the dried root of Scutellaria baicalensis, an herb of the Labiatae family, with a bitter taste and a cold medicinal material. The active ingredient of Scutellaria baicalensis is mainly baicalin, which has a wide range of biological activities, such as anti-inflammation, anti-allergic reaction, antipyretic, detoxification, anti-pathogen, sedative and antihypertensive. However, baicalin has poor solubility, low oral bioavailability, and unstable drug efficacy. These properties limit the wide application of baicalin. [0003] Transdermal drug delivery refers to the drug delivery method in which the skin is absorbed into the body and reaches an effective blood concentration to achieve treatment or prevention of diseases. This way of administr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K9/127A61K47/22A61K47/38A61K31/7048A61K36/539A61P29/00A61P37/08A61P39/02A61P31/00A61P25/20A61P9/12
CPCA61K9/06A61K9/127A61K47/22A61K47/38A61K31/7048A61K36/539A61P29/00A61P37/08A61P39/02A61P31/00A61P25/20A61P9/12Y02A50/30
Inventor 吕凤娇谢晓兰孙丽丹刘瑞花翁文婷高平章
Owner QUANZHOU NORMAL UNIV
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