Dual-mode tumor imaging nanoprobe suitable for mri/nir-ii, preparation method and application
A nano-probe and tumor imaging technology, which is applied in the field of biomedical materials, can solve the problems of complex toxicity of dual-modal imaging materials, and achieve high industrial application prospects, good biocompatibility, and high reaction efficiency.
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Embodiment 1
[0046] A dual-modal tumor imaging nanoprobe suitable for MRI / NIR-II, composed of apo-lactoferrin, Mn 2+ . 2+ And it is coupled with the amino group on PEG and the carboxyl group on CH1055, and the molecules are cross-linked through aldehyde groups and amino groups.
[0047] figure 1 It is a schematic structural diagram of the dual-mode tumor imaging nanoprobe of the present invention; wherein, for lactoferrin, for manganese ions, for the CH1055 molecule, for PEG, For iron ions; figure 2 It is a schematic structural diagram of the synthesis process of the dual-modal tumor imaging nanoprobe of the present invention.
[0048] The particle size of the dual-mode tumor imaging nanoprobe is 150-170nm.
Embodiment 2
[0050] A method for preparing a dual-mode tumor imaging nanoprobe suitable for MRI / NIR-II, comprising the following steps:
[0051] (1) The raw material lactoferrin is prepared into an aqueous solution of a certain concentration, and the iron ion on the lactoferrin is removed by dialysis at 15° C. with an acid solution with a pH of 2, and freeze-dried to obtain apo-lactoferrin Apo-Lf;
[0052] (2) Dissolve the Apo-Lf obtained in step (1) in a mixed solution of a certain amount of manganese salt and carbonate, stir at 20°C until the solution becomes a suspension, and then use a certain concentration of carbonate solution Dialyze with water to remove impurities, and freeze-dry to obtain iron-free plus manganese lactoferrin Lf-Mn 2+ ;
[0053] (3) In an anhydrous and oxygen-free environment, a certain amount of PEG is dissolved in DMF, and a certain amount of EDCI, NHS, and the Lf-Mn obtained in the step (2) are added successively. 2+ , DIPEA was stirred at room temperature unt...
Embodiment 3
[0063] A method for preparing a dual-mode tumor imaging nanoprobe suitable for MRI / NIR-II, comprising the following steps:
[0064] (1) The raw material lactoferrin is prepared into an aqueous solution of a certain concentration, and the iron ion on the lactoferrin is removed by dialysis at 25° C. with an acid solution having a pH of 6, and freeze-dried to obtain apo-lactoferrin Apo-Lf;
[0065] (2) Dissolve the Apo-Lf obtained in step (1) in a mixed solution of a certain amount of manganese salt and carbonate, stir at 30°C until the solution becomes a suspension, and then use a certain concentration of carbonate solution Dialyze with water to remove impurities, and freeze-dry to obtain iron-free plus manganese lactoferrin Lf-Mn 2+ ;
[0066] (3) In an anhydrous and oxygen-free environment, a certain amount of PEG is dissolved in DMF, and a certain amount of EDCI, NHS, and the Lf-Mn obtained in the step (2) are added successively. 2+ , DIPEA was stirred at room temperature u...
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