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Black foul water body dephosphorization strain breeding method capable of avoiding secondary pollution

A technology of secondary pollution and cultivation method, applied in the direction of bacteria, adding compounds to stimulate growth, etc., can solve the problems of low survival rate of phosphorus removal bacteria, inability to achieve the survival rate of phosphorus removal bacteria, secondary pollution, etc.

Pending Publication Date: 2019-09-17
江西调水人生态环境工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies in the prior art, the present invention provides a method for cultivating phosphorus-removing bacteria in black and odorous water that avoids secondary pollution, and solves the problem that the survival rate of phosphorus-removing bacteria obtained by the existing cultivation method is low, and it is impossible to realize the first generation of phosphorus-removing bacteria. Targeted experimental cultivation of the strains, so that the strains have a high resistance, and then put into the biochemical culture tank for practical cultivation, it is impossible to make the bred strains can reproduce in large quantities under harsh living conditions Therefore, it is impossible to improve the survival rate of phosphorus-removing bacteria in actual feeding and use. At the same time, the existing cultivation method has a low survival rate of phosphorus-removing bacteria, so that the phosphorus content in the cultivated residue is too high, and it will cause pollution when discharged into the environment. Cause secondary pollution, it is impossible to improve the survival rate of phosphorus removal bacteria in the cultivation of bacteria, so that the phosphorus in the culture pool is absorbed in large quantities, so as to prevent the culture from causing secondary pollution to the environment.

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  • Black foul water body dephosphorization strain breeding method capable of avoiding secondary pollution

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Experimental program
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Embodiment 1

[0024] S1. Preparation of the culture medium of the first-generation bacteria: firstly, select 15 parts of phosphate iron ore powder and use a grinder to grind and sieve to obtain powder particles with a particle size of 100 mesh, then add clean water and stir for 25 minutes at a speed of 250r / min. Obtain the phosphorite solution, then add 25 parts of sucrose, 7 parts of dipotassium hydrogen phosphate, 7 parts of magnesium sulfate and 7 parts of ammonium nitrate into the phosphoside solution successively, with the rotating speed being 450r / min, stirring for 1.5h, Obtain the culture of the first-generation strains, then add 3 parts of pH regulator to the culture of the first-generation strains, adjust the pH of the medium to 7, and then obtain a medium suitable for the growth of the strains;

[0025] S2, the selection and inoculation of the first-generation phosphorus-removing bacteria: select the bacterial liquid from the strain room, and then put the first-generation bacterial...

Embodiment 2

[0030] S1. Preparation of the culture medium of the first-generation bacteria: firstly, select 10 parts of phosphate iron ore powder and use a grinder to grind and sieve to obtain powder particles with a particle size of 100 mesh, then add water and stir for 20 minutes at a speed of 200r / min. Obtain the phosphinic ore solution, then add 20 parts of sucrose, 5 parts of dipotassium hydrogen phosphate, 5 parts of magnesium sulfate and 5 parts of ammonium nitrate into the phosphofertic ore solution successively, and stir for 1 hour at a rotating speed of 400r / min to obtain Cultivate the first-generation strains, and then add 1 part of pH regulator to the culture of the first-generation strains to adjust the pH of the medium to 6.6 to obtain a medium suitable for the growth of the strains;

[0031] S2, the selection and inoculation of the first-generation phosphorus-removing bacteria: select the bacterial liquid from the strain room, and then put the first-generation bacterial cultu...

Embodiment 3

[0036] S1. Preparation of the culture medium of the first-generation bacteria: first, select 20 parts of phosphoferite powder and use a grinder to grind and sieve to obtain powder particles with a particle size of 110 mesh, then add water and stir for 30 minutes at a speed of 300r / min. Obtain the phosphinic ore solution, then add 30 parts of sucrose, 10 parts of dipotassium hydrogen phosphate, 10 parts of magnesium sulfate and 10 parts of ammonium nitrate into the phosphofertic ore solution successively, and stir for 2 hours at a rotating speed of 500r / min to obtain Cultivation of the first-generation strains, and then adding 5 parts of pH regulator to the culture of the first-generation strains to adjust the pH of the culture medium to 7.5 to obtain a culture medium suitable for the growth of the strains;

[0037] S2, the selection and inoculation of the first-generation phosphorus-removing bacteria: select the bacterial liquid from the strain room, and then put the first-gene...

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Abstract

The invention discloses a black foul water body dephosphorization strain breeding method capable of avoiding secondary pollution. The black foul water body dephosphorization strain breeding method specifically comprises the following steps of S1, preparing culture mediums of primary strains; S2, performing selection and inoculation of primary dephosphorization strains; S3, performing pretreatment on polluted water bodies; S4, putting strains for performing integrated breeding of black foul water bodies; and S5, taking bacteria for detection and preservation. The invention relates to the technical field of environment government. Through the adoption of the black foul water body dephosphorization strain breeding method capable of avoiding secondary pollution disclosed by the invention, the purpose that bred strains can be in mass propagation under the environment severe in requirements for survival conditions can be achieved, the survival rate of dephosphorization bacteria can be increased during breeding of the black foul water body dephosphorization strains, massive phosphorus components of black foul water bodies in a culture pond are absorbed to prevent the generation of the secondary pollution situation of the environment caused by cultures, and the cultured dephosphorization bacteria are high in adaptability, extremely high in tolerance on complex substances in the system, stable in bioactivity and high in shock resistance to complex changes of water quality.

Description

technical field [0001] The invention relates to the technical field of environmental treatment, in particular to a method for cultivating phosphorus-removing bacteria in black and odorous water bodies to avoid secondary pollution. Background technique [0002] Dephosphorus bacteria are a special kind of bacteria in the traditional activated sludge process. Under aerobic conditions, they can absorb phosphorus in sewage into the body in excess, making the phosphorus content in the body several times higher than that of ordinary bacteria. , this type of bacteria is widely used in biological phosphorus removal. It is generally believed that phosphorus removal bacteria can be divided into two types, facultative anaerobic denitrification phosphorus removal bacteria and aerobic phosphorus removal bacteria. Among them, denitrification phosphorus removal bacteria can use oxygen Or nitrate as the electron acceptor, while aerobic phosphorus removal bacteria can only use oxygen as the e...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/38
CPCC12N1/20C12N1/38
Inventor 陶妹英曾庆辉汪辉
Owner 江西调水人生态环境工程有限公司