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A bioreactor for animal cell culture

An animal cell culture and bioreactor technology, which is applied in specific-purpose bioreactor/fermenter, bioreactor/fermenter combination, tissue cell/virus culture device, etc., can solve uneven mixing, dead ends, etc. The problems of high filtration cost and high shear force can achieve the effect of realizing industrial production, reducing solid industrial waste and improving activity.

Active Publication Date: 2020-08-04
迪必尔生物工程(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The stirring process of the bioreactor for animal cell culture in the prior art has problems such as large shear force, uneven mixing, and high cost of dead-end filtration

Method used

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  • A bioreactor for animal cell culture
  • A bioreactor for animal cell culture
  • A bioreactor for animal cell culture

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] by figure 1 As an example, the present invention provides a bioreactor for animal cell culture, including a reaction tank 1 and a piston cylinder 2 coaxial with the tank body inside the reaction tank 1, and a piston 3 inside the piston cylinder 2 , the piston 3 is connected with the eccentric wheel 4 through the transmission device, the piston cylinder 2 is provided with an air outlet 6, and it is located on the top of the piston cylinder 2, the air outlet 6 is connected with an air outlet pipe, and the highest point of the air outlet pipe is preferably higher than the reaction The height of the liquid level in the device is equipped with a dish gas distributor device 5 at the bottom of the reaction tank body 1, and an outlet of the reaction tank body 1 is connected with the inlet of the continuous centrifuge 8 through a pump 9, and one of the continuous centrifuge 8 The outlet is connected to the downstream process, and the other outlet is connected to the reaction tan...

Embodiment 2

[0058] For a kind of bioreactor for animal cell culture described in embodiment 1, when reaction tank body 1 volume is 20m 3 When the frequency of the piston movement is 1Hz, the internal mixing state is simulated. When the butterfly gas distributor is not opened, the simulation results of the liquid flow distribution and liquid streamline inside the reaction tank 1 are shown in Figure 4 , when there is no stirring in the present invention, and when the piston movement is used alone to mix, the liquid flow rate is low, and a dead zone where the liquid hardly flows will appear at the bottom of the reaction tank 1, and the particles in the reactor system will be deposited here. affect the cell culture process. When the butterfly gas distributor 5 is opened, the gas enters the reaction tank 1 from the gas distributor 5, and the disturbance caused by the bubble movement can eliminate the dead zone, such as Figure 5 shown. Compared Figure 4 and Figure 5 It can be seen that ...

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Abstract

The invention discloses a biological reactor for animal cell culture and belongs to the field of cell culture. The biological reactor is characterized in that liquid flow of the reactor is directly promoted by reciprocating motion of a piston instead of rotation of an impeller, and the demand of rapid mixing under the condition of extremely low shear force is met; meanwhile, a disc type gas distributor is installed at the lowest part of the reactor, so that the air flow drives liquid to flow, and cells and microcarriers are ensured not to deposit; in addition, continuous centrifugation is used to concentrate the cells and excrete metabolic waste, and harm caused by cross-flow filtration on the cells is avoided. The loss caused by the shear force to the animal cells is minimized, and the activity, growth characteristics and yield of the cells can be improved; the biological reactor is suitable for continuous operation, long-time operation and repeated operation, equipment cost is reduced, generation of solid industrial waste is reduced, and secondary pollution is not caused.

Description

technical field [0001] The invention relates to a bioreactor for animal cell culture, especially suitable for high-density culture of medium-scale animal cells, especially as a seed reactor or expansion reactor for high-density and large-scale culture of edible animal muscle cells , belonging to the field of cell culture. Background technique [0002] Bioreactors are used to provide the cultured cells with an optimal environment suitable for their growth or product production. These environmental parameters include but are not limited to dissolved oxygen (DO), partial pressure of carbon dioxide (pCO 2 ), acidity (pH), osmotic pressure, ionic strength, substrate concentration, temperature, pressure, shear stress, etc. In the prior art, the way to keep these parameters constant (meaning not changing with time, or changing with time according to a specified curve) and uniform (meaning not changing with space, or changing with space according to a specified form) is generally t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/02C12M1/04C12M3/00C12M1/00
CPCC12M25/00C12M27/02C12M29/06
Inventor 李雪良陈坚堵国成李江华周景文张国强赵鑫锐孙秀兰
Owner 迪必尔生物工程(上海)有限公司