Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for detecting residual validamycin on rice

A technology for Jinggangmycin and rice, which is applied in the field of detecting Jinggangmycin residues on rice, can solve the problems of accuracy and representativeness deviation of detection results, inability to analyze by gas chromatography, difficult gasification, etc., and achieve accurate measurement results and adaptability. Wide and efficient extraction effect

Inactive Publication Date: 2019-09-24
江苏恒生检测有限公司
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, for some target pesticides that are difficult to extract from crops, it is often impossible to eliminate matrix interference or achieve a good extraction effect with general methods, which leads to a greatly reduced detection effect and cannot achieve accurate determination
Moreover, Jinggangmycin A is an aminoglycoside compound, which contains multiple polar groups in the molecule, is difficult to gasify, and cannot be directly analyzed by gas chromatography. Generally, Jinggangmycin residues on rice can only be detected through soil, field water, and plants. , brown rice or rice husk and other single substrates, there are some deviations in the accuracy and representativeness of the final test results

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Detection of Jinggangmycin Residues in Field Water

[0023] Treatment of field water samples: directly measure the field water sample solution;

[0024] Concentration of field water sample solution: adjust the pH value of the field water sample solution to 2-3, then use a chromatographic column for adsorption and collection, sequentially wash with pH 3.5 phosphate buffer solution and ethanol to remove impurities, and then use eluent For elution, the eluent is a mixed solution of 1mol / L ammonia water and ethanol, the volume ratio of ammonia water and ethanol is 4:1, and the elution rate is 0.8mL / min, and the obtained eluent is concentrated by a rotary evaporator to nearly dryness , and then blow dry with nitrogen or air, quantitatively add 2 mL of pH 7.0 disodium hydrogen phosphate-potassium hydrogen phosphate buffer solution and ultrasonically dissolve to obtain a sample concentrate of Tianshui;

[0025] Detection: After the sample concentrate of Tianshui was...

Embodiment 2

[0026] Jinggangmycin residual detection in the plant of embodiment 2

[0027] Treatment of plant samples: Weigh 2.5g of plant samples, place them in a 50mL centrifuge tube, add 15mL of water to infiltrate, then ultrasonicate for 60min, then add 10mL of methanol to extract by vortexing and oscillating for 60min, then centrifuge at 4000r / min for 5min and then absorb For the supernatant, repeat the above steps twice, and combine the supernatant to obtain the sample solution of the plant;

[0028] Concentration of the plant sample solution: Adjust the pH of the plant sample solution to 2-3, then use a chromatographic column for adsorption and collection, sequentially wash with pH 3.5 phosphate buffer solution and ethanol to remove impurities, and then wash with eluent Eluting, the eluent is a mixed solution of 1mol / L ammonia water and ethanol, the volume ratio of ammonia water and ethanol is 4:1, and the elution rate is 0.8mL / min. Blow dry with nitrogen or air, quantitatively add...

Embodiment 3

[0032] Example 3 Detection of Jinggangmycin residues in rice husk

[0033] Treatment of rice husk samples: Weigh 2.5g of rice husk samples, place them in a 50mL centrifuge tube, add 15mL of water to infiltrate them, then ultrasonicate for 60min, then add 10mL of methanol to extract by vortexing and shaking for 60min, and then centrifuge at a speed of 4000r / min for 5min Then draw the supernatant, repeat the above steps 2 times, and combine the supernatant to obtain the sample solution of rice husk;

[0034]Concentration of the rice husk sample solution: adjust the pH value of the rice husk sample solution to 2-3, then use a chromatographic column for adsorption and collection, successively wash with pH 3.5 phosphate buffer solution and ethanol to remove impurities, and then wash with eluent For elution, the eluent is a mixed solution of 1mol / L ammonia water and ethanol, the volume ratio of ammonia water and ethanol is 4:1, and the elution rate is 0.8mL / min, and the obtained elu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for detecting residual validamycin on rice, comprising the following steps of S1, sample processing: directly measuring a field water sample solution; weighing a certain number of plant, rice hull, soil or brown rice samples, placing the sample in a centrifuge tube, adding water to soak the sample, performing a ultrasonic treatment, adding methanol to perform orbital shaking extraction, then performing a centrifuging treatment, taking the supernatant, repeating the step 1 to 3 times, combining the supernatant to obtain a sample solution; S2, sample solution concentration: subjecting each sample solution to adsorption collection by a chromatographic column, and concentrating an eluent by a rotary evaporator; S3: detection: detecting the filtered concentrated liquid of the field water sample by high-performance liquid chromatography, and detecting the filtered concentrated liquid of the plant, rice hull, soil or brown rice samples. The method achieves high accuracy, good precision, a low detection limit and a recovery rate of 100+ / -30%, and satisfies the technical requirements of pesticide residue analysis and detection.

Description

technical field [0001] The invention belongs to the technical field of pesticide residue analysis, and in particular relates to a method for detecting Jinggangmycin residue on rice. Background technique [0002] Jinggangmycin is a water-soluble antibiotic produced by Streptomyces waterus Jinggang variety, and its active ingredients are A, B, C, D, E, F, a total of 6 components, of which Jinggangmycin A is the main active ingredient. The specification and quality of the product is also represented by the content of Jinggangmycin A. Jinggangmycin is a biogenic fungicide independently developed by my country. It is mainly used to prevent and control rice sheath blight. It has strong systemic properties and has both protective and therapeutic effects. Drug varieties. At present, for some target pesticides that are difficult to extract from crops, it is often impossible to eliminate matrix interference or achieve a good extraction effect with general methods, which leads to a gr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 查欣欣
Owner 江苏恒生检测有限公司