Boar sperm preservative as well as preparation method and application thereof

A technology of pig sperm and preservative, applied in the field of animal husbandry, can solve problems such as microbial pollution and affecting the quality of semen preservation, achieve the effects of inhibiting the number of bacteria, improving sperm vitality and fertilization ability, and slowing down the phenomenon of hypoxia

Pending Publication Date: 2019-09-27
INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Diluent raw materials are essential components for semen preservation, but in the production process, due to environmental...

Method used

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  • Boar sperm preservative as well as preparation method and application thereof
  • Boar sperm preservative as well as preparation method and application thereof
  • Boar sperm preservative as well as preparation method and application thereof

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preparation example Construction

[0022] The present invention provides the preparation method of above-mentioned pig sperm preservative, comprises the steps:

[0023] (1) Sodium citrate, citric acid and trishydroxymethylaminomethane are pulverized respectively to obtain pulverized sodium citrate, pulverized citric acid and pulverized trishydroxymethylaminomethane;

[0024] (2) the sodium citrate of described pulverization, the citric acid of pulverization, trishydroxymethyl aminomethane of pulverization, glucose, sodium bicarbonate, EDTA and sodium carboxymethylcellulose are sterilized respectively, obtain the sodium citrate after disinfection , sterilized citric acid, sterilized Tris, sterilized glucose, sterilized sodium bicarbonate, sterilized EDTA and sterilized sodium carboxymethyl cellulose;

[0025] (3) the sodium citrate after the disinfection, the citric acid after the disinfection, the tris after the disinfection, the glucose after the disinfection, the sodium bicarbonate after the disinfection, the...

Embodiment 1

[0035] (1) Accurately weigh 1 to 200 parts of porcine sperm preservative composition, each part of porcine sperm preservative composition includes: glucose 11.5g, sodium bicarbonate 1.25g, sodium citrate 11.7g, EDTA 2.3g, citric acid 4.1g, Tris (Tris) 6.5g, L-cysteine ​​0.15g, Levofloxacin 0.1g, Carmellose Sodium 4g, Bovine Serum Albumin 2g, Banqing Baidu Oral solution 1ml.

[0036] (2) Grind sodium citrate, citric acid and tris (Tris) with a soybean milk grinder for 10-20 minutes.

[0037] (3) The crushed sodium citrate, citric acid and Tris (Tris), glucose, sodium bicarbonate, EDTA and sodium carboxymethylcellulose were dry-heat sterilized (at 70°C) 1 hour.

[0038] (4) The above-mentioned sterilized medicine is mixed with L-cysteine, levofloxacin, bovine serum albumin and Banqing Baidu oral liquid, and poured into a sterilized small mixer (230L, 220v small Mixer, the maximum mixing capacity is 150 catties, the rotation speed is 40-50r / min), and the stirring time is at lea...

Embodiment 2

[0041] Preparation of Porcine Sperm Diluent

[0042] The following medicines: glucose 11.5g, sodium bicarbonate 1.25g, sodium citrate 11.7g, EDTA 2.3g, citric acid 4.1g, Tris (Tris) 6.5g, L-cysteine ​​0.15g, Levofloxacin 0.1g, carboxymethylcellulose sodium 4g, bovine serum albumin 2g, Banqingbaidu oral liquid 1ml, put in a 1000mL sterilized bottle, and then add 1000mL double distilled water into the sterilized bottle, Finally, heat, dissolve and equilibrate in a water bath at 35°C for more than 1 hour before use.

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Abstract

The invention provides a boar sperm preservative as well as a preparation method and application thereof, and belongs to the technical field of livestock breeding. The boar sperm preservative provided by the invention is prepared from glucose, sodium hydrogen carbonate, sodium citrate, EDTA, citric acid, trihydroxymethyl aminomethane, L-cysteine, levofloxacin, sodium carboxymethylcellulose, bovine serum albumin and Banqing toxin-removing oral liquid. Compared with a conventional diluent Zorlesco, the boar sperm preservative provided by the invention has a better preservation effect of boar sperms, can improve the antibacterial ability of boar semen, and prolongs the storage time of the boar sperms.

Description

technical field [0001] The invention relates to the technical field of animal husbandry, in particular to a pig sperm preservation agent and its preparation method and application. Background technique [0002] In large-scale pig production, the main cause of the abnormally high culling rate of sows in pig farms is reproductive diseases, and more than 50% of reproductive diseases are caused by endometritis. According to reports, compared with normal sows, sows with endometritis had a corresponding increase of 15.81 days and 30.67 days in the first mating days and 30.67 days in postpartum conception days, and a 21.36% decrease in conception rate during estrus. The increase was 0.51 times, which had a huge impact on the economic benefits of the pig farm. [0003] Studies have shown that bacteria in the diluent or semen is the primary reason for the expansion and spread of metritis. The diluent is an essential component in the preservation of semen. It can dilute, buffer, nou...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 张家庆邢宝松王献伟陈俊峰王晓锋白献晓牛刚马强高彬文任巧玲白红杰王璟徐照学
Owner INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE HENAN ACAD OF AGRI SCI
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