Boar sperm preservative as well as preparation method and application thereof
A technology of pig sperm and preservative, applied in the field of animal husbandry, can solve problems such as microbial pollution and affecting the quality of semen preservation, achieve the effects of inhibiting the number of bacteria, improving sperm vitality and fertilization ability, and slowing down the phenomenon of hypoxia
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[0022] The present invention provides the preparation method of above-mentioned pig sperm preservative, comprises the steps:
[0023] (1) Sodium citrate, citric acid and trishydroxymethylaminomethane are pulverized respectively to obtain pulverized sodium citrate, pulverized citric acid and pulverized trishydroxymethylaminomethane;
[0024] (2) the sodium citrate of described pulverization, the citric acid of pulverization, trishydroxymethyl aminomethane of pulverization, glucose, sodium bicarbonate, EDTA and sodium carboxymethylcellulose are sterilized respectively, obtain the sodium citrate after disinfection , sterilized citric acid, sterilized Tris, sterilized glucose, sterilized sodium bicarbonate, sterilized EDTA and sterilized sodium carboxymethyl cellulose;
[0025] (3) the sodium citrate after the disinfection, the citric acid after the disinfection, the tris after the disinfection, the glucose after the disinfection, the sodium bicarbonate after the disinfection, the...
Embodiment 1
[0035] (1) Accurately weigh 1 to 200 parts of porcine sperm preservative composition, each part of porcine sperm preservative composition includes: glucose 11.5g, sodium bicarbonate 1.25g, sodium citrate 11.7g, EDTA 2.3g, citric acid 4.1g, Tris (Tris) 6.5g, L-cysteine 0.15g, Levofloxacin 0.1g, Carmellose Sodium 4g, Bovine Serum Albumin 2g, Banqing Baidu Oral solution 1ml.
[0036] (2) Grind sodium citrate, citric acid and tris (Tris) with a soybean milk grinder for 10-20 minutes.
[0037] (3) The crushed sodium citrate, citric acid and Tris (Tris), glucose, sodium bicarbonate, EDTA and sodium carboxymethylcellulose were dry-heat sterilized (at 70°C) 1 hour.
[0038] (4) The above-mentioned sterilized medicine is mixed with L-cysteine, levofloxacin, bovine serum albumin and Banqing Baidu oral liquid, and poured into a sterilized small mixer (230L, 220v small Mixer, the maximum mixing capacity is 150 catties, the rotation speed is 40-50r / min), and the stirring time is at lea...
Embodiment 2
[0041] Preparation of Porcine Sperm Diluent
[0042] The following medicines: glucose 11.5g, sodium bicarbonate 1.25g, sodium citrate 11.7g, EDTA 2.3g, citric acid 4.1g, Tris (Tris) 6.5g, L-cysteine 0.15g, Levofloxacin 0.1g, carboxymethylcellulose sodium 4g, bovine serum albumin 2g, Banqingbaidu oral liquid 1ml, put in a 1000mL sterilized bottle, and then add 1000mL double distilled water into the sterilized bottle, Finally, heat, dissolve and equilibrate in a water bath at 35°C for more than 1 hour before use.
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