A kind of gold nanoprobe for detecting benzoyl peroxide and its preparation method and use

A technology of benzoyl peroxide and nano-probes, which is applied in the direction of material analysis by observing the influence of chemical indicators, metal processing equipment, and analysis by making materials undergo chemical reactions, which can solve false positives, interference, etc. problem, to achieve high accuracy, good selectivity and sensitivity, and good sensitivity

Active Publication Date: 2020-02-18
BEIJING CENT FOR DISEASE PREVENTION & CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, aggregate-induced color changes are easily disturbed by various microenvironmental factors, salt concentrations, and interfering substances, thus having strong false positive results

Method used

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  • A kind of gold nanoprobe for detecting benzoyl peroxide and its preparation method and use
  • A kind of gold nanoprobe for detecting benzoyl peroxide and its preparation method and use
  • A kind of gold nanoprobe for detecting benzoyl peroxide and its preparation method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 14

[0065] Example 1 4-Mercaptophenylboronic acid-modified gold nanoparticles (MPBA-AuNPs 65 ) preparation and characterization

[0066] S1: Prepare a citrate-stabilized AuNPs sol according to the method disclosed in the literature Grabar, K.C.; Freeman, R.G.; Hommer, M.B.; Natan, M.J., Anal.Chem. 1995, 67, 735-743.

[0067] S2: TA ethanol solution (0.2mL, 0.1mol L -1 ) and MPBA ethanol solution (0.8mL, 0.1mol L -1 ) into the reaction flask and mix evenly, and dropwise added the citrate-stabilized AuNPs sol (100mL, 10nmol L -1 , pH9.0), magnetically stirred for 2 hours;

[0068] S3: Centrifuge the AuNPs sol obtained in the previous step (15700g, 10 minutes), discard the supernatant, and resuspend the deposited AuNPs in ultrapure water to obtain MPBA-AuNPs 65 .

[0069] The shape and particle size of gold nanoparticles were observed by transmission electron microscopy, and the results were as follows: figure 2 As shown, MPBA-AuNPs 65 The shape is uniform and the particle ...

Embodiment 2

[0070] Example 2 Dopamine-modified gold nanoparticles (DPA-AuNPs 12 ) preparation and characterization

[0071] S1: According to the literature Bastus, N.G.; Comenge, J.; Puntes, V., Langmuir 2011, 27, 11098–11105. The method of kinetically controlled seed growth was prepared to prepare citrate-stabilized AuNPs, and the particle size of AuNPs was about 12nm;

[0072] S2: TA ethanol solution (0.2mL, 0.1mol L- 1 ) was added dropwise to the citrate-stabilized AuNPs sol (100 mL, 10 nmol L- 1 , pH 9.0), incubated at room temperature for 2 hours, centrifuged, and resuspended the deposited AuNPs in ultrapure water to obtain TA-modified AuNPs;

[0073] S3: The TA-modified AuNPs obtained in the previous step were mixed with sulfo-NHS (10mg mL -1 ) and EDC (20mg mL -1 ) in 1.0mL 10mM MES (morpholinoethanesulfonic acid) buffer for 30 minutes, remove the supernatant, add DPA (10mg) dispersed in 10mM PBS solution, react at room temperature for 2 hours, and use 0.5% glycine quenched ...

Embodiment 3

[0075] Example 3 Gold nanoparticle probes (BE-AuNPs 12 / 65 ) preparation and characterization

[0076] S1: MPBA-AuNPs prepared in Example 1 65 (1.0OD, 545nm) and the DPA-AuNPs prepared in Example 2 12 (2.0OD, 523nm) dispersed in NaHCO 3 (pH 8.5 10mL, 1mmol L -1 ) buffer solution, incubated for 2 hours, a boronate bond was formed between the two gold nanoparticles;

[0077] S2: Centrifuge (6000g, 10 minutes), discard the supernatant, wash the sediment with water, and redisperse in NaHCO 3 Solution (pH8.6, 10mL, 1mmol L -1 ), gold nanoprobes (BE-AuNPs 12 / 65 ).

[0078] MPBA-AuNPs 65 and DPA-AuNPs 12 After physical mixing in deionized water, the morphology and particle size of gold nanoparticles were observed by transmission electron microscopy, and the results were as follows: Figure 4 As shown, MPBA-AuNPs with larger particle size 65 and DPA-AuNPs with smaller particle size 12 Separately dispersed without any connection phenomenon; BE-AuNPs 12 / 65 Transmission ele...

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Abstract

The invention discloses a gold nanoprobe for detecting benzoyl peroxide and its preparation method and application. The nanoprobe is a gold nanoparticle assembly with a "nuclear-satellite" structure, modified with a boronic acid group The gold nanoparticles of the compounds act as "cores" (C‑AuNPs), and the gold nanoparticles of compounds modified to react with boronic acid groups to form boronate serve as "satellites" (S‑AuNPs), and the gold nanoparticles of the "cores" Nanoparticles are connected to the gold nanoparticles of the "satellite" by forming boronate groups. The nanoprobes can be used for colorimetric detection of BPO at the nanomolar level without complicated instruments, and the detection speed is fast and the accuracy is good , High sensitivity. Without knowing the specific concentration of BPO, you can qualitatively / semi-quantitatively judge whether the sample contains BPO according to the color change, as well as the approximate content range, and judge whether the BPO content in the sample exceeds the standard.

Description

technical field [0001] The invention belongs to the field of food safety detection, and in particular relates to a gold nanoprobe for detecting benzoyl peroxide, a preparation method and application thereof. Background technique [0002] Benzoyl peroxide (BPO) is a highly active oxidizing agent. Due to its good oxidative bleaching performance, it was first used for oil bleaching, and then widely used as an additive for wheat flour. It not only has a bleaching effect, but also can greatly shorten the flour the maturation cycle. The mechanism of benzoyl peroxide bleaching flour is: benzoyl peroxide reacts under the action of water and enzymes in the flour, releasing active oxygen to oxidize a very small number of unsaturated fat-soluble pigments with conjugated double bonds in the flour , that is, β-carotene and other colored ingredients reduce the pigment content in the flour and increase the whiteness. [0003] However, it was later discovered that adding excessive amounts...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B22F1/00B22F1/02G01N21/31G01N21/78
CPCG01N21/78G01N21/31B22F1/07B22F1/102B22F1/14B22F1/054
Inventor 孙洁芳邵兵张晶
Owner BEIJING CENT FOR DISEASE PREVENTION & CONTROL
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