Preparation method of 7-aminocephalosporanic acid

Abstract

The invention relates to a preparation method of 7-aminocephalosporanic acid. The method comprises steps as follows: a cephalosporin C filtrate is separated from fermentation liquor in a membrane filtration manner; cephalosporin C is extracted from the filtrate by adopting an organic solvent which is immiscible with water, and cephalosporin C extract liquor is obtained; cephalosporin C in the extract liquor is subjected to reverse extraction into aqueous phase, the residual organic solvent is removed by reduced-pressure distillation, and an aqueous cephalosporin C sodium salt solution with high concentration is obtained; finally, after cephalosporin C is subjected to enzyme hydrolysis, crystallization, filtering, washing and drying, 7-aminocephalosporanic acid meeting the quality standard can be obtained. With the adoption of extraction and reverse extraction, impurities in the aqueous cephalosporin C sodium salt solution are reduced substantially, and the quality of the aqueous cephalosporin C sodium salt solution is improved, so that high-quality 7-aminocephalosporanic acid is obtained by enzyme hydrolysis.

Description

technical field

[0001] The invention belongs to the technical field of pharmacy and relates to a preparation method of 7-aminocephalosporanic acid. Background technique

[0002] 7-amino-cephalosporanic acid (7-Amino-Cephalosporanic acid), the structural formula is as follows:

[0003]

[0004] 7-Aminocephalosporanic acid, referred to as 7-ACA, is the most commonly used core in cephalosporins. It has two active groups, the 3-position acetoxy group and the 7-position amino group. Different side chains are connected on the group to form cephalosporin antibiotics with different properties. Therefore, 7-ACA is a very important intermediate of cephalosporin (β-lactam) antibiotics. The cephalosporin antibiotics can effectively inhibit the synthesis of bacterial cell walls, but human cells have no cell walls, so they have excellent selectivity, good safety, and strong bactericidal power. They are currently the best type of antibiotics used clinically. occupy a very important po...

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