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Novel material based on natural diatom shell and phycobiliprotein and application

A technology of phycobiliproteins and new materials, which is applied in the direction of non-active ingredients medical preparations, medical preparations containing active ingredients, wave energy or particle radiation treatment materials, etc., can solve the problem of weak photosensitivity effect and non-structural phycocyanin Very stable, application restrictions and other issues, to achieve the effect of strong stability

Active Publication Date: 2019-10-01
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the structure of phycocyanin is not very stable, and the photosensitization effect is weak, so its application as a photosensitizer is greatly limited.

Method used

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  • Novel material based on natural diatom shell and phycobiliprotein and application
  • Novel material based on natural diatom shell and phycobiliprotein and application
  • Novel material based on natural diatom shell and phycobiliprotein and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042]A method for preparing a novel material based on natural diatom shells and phycocyanin, comprising the steps of:

[0043] (1) Prepare f / 2 seawater medium and add 30 mg / L sodium silicate to adjust the pH to 7.8-8.0. Inoculate the diatom algae species of the genus Nanofrustulum, set the culture temperature of the light incubator at 25°C, 12h, 600Lux continuous light, 12h dark treatment, and regularly collect well-growing diatoms by precipitation method (diatom optical microscope picture See figure 1 ).

[0044] (2) Settling the harvested diatom algae in a beaker for 6 hours, absorbing the supernatant, adding 10 mL of distilled water and blowing, then standing for 4 hours, absorbing the supernatant, adding 100 mL of distilled water, and standing at room temperature for four to five days, Aspirate the supernatant, add 35mL hydrogen peroxide, and treat in a water bath at 90°C for 4 hours in the dark; the pure silicon shell obtained by removing the organic matter is obtained...

Embodiment 2

[0053] A method for preparing a novel material based on natural diatom shells and phycocyanin, comprising the steps of:

[0054] (1) Prepare f / 2 seawater medium and add 30 mg / L sodium silicate to adjust the pH to 7.8-8.0. Inoculate the diatom species and set the culture temperature of the light incubator at 25°C, 12h, 600Lux continuous light, 12h dark treatment, and regularly collect diatoms by precipitation method.

[0055] (2) Settling the harvested diatom algae in a beaker for 6 hours, absorbing the supernatant, adding 10 mL of distilled water and blowing, then standing for 4 hours, absorbing the supernatant, adding 100 mL of distilled water, and standing at room temperature for four to five days, Aspirate the supernatant, add 35mL hydrogen peroxide, and treat in a 90°C water bath in the dark for 4 hours; the organic matter is removed to obtain a pure silicon shell.

[0056] (3) Piranha solution (H 2 o 2 °CH 2 SO 4 = 1°C 6 mixing) at 80°C for 30 minutes, and washed twi...

Embodiment 3

[0064] A method for preparing a novel material based on natural diatom shells and phycocyanin, comprising the steps of:

[0065] (1) Prepare f / 2 seawater medium and add 30 mg / L sodium silicate to adjust the pH to 7.8-8.0. Inoculate the diatom species and set the culture temperature of the light incubator at 25°C, 12h, 600Lux continuous light, 12h dark treatment, and regularly collect diatoms by precipitation method.

[0066] (2) Settling the harvested diatom algae in a beaker for 10 hours, absorbing the supernatant, adding 100 mL of distilled water and blowing, then standing for 6 hours, absorbing the supernatant, adding 200 mL of distilled water, and standing at room temperature for four to five days, Aspirate the supernatant, add 85mL hydrogen peroxide, and treat in a water bath at 80°C in the dark for 4 hours; the organic matter is removed to obtain a pure silicon shell.

[0067] (3) Piranha solution (H 2 o 2 :H 2 SO 4 =1:8 mixing) at 80°C for 50 minutes, and washed tw...

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Abstract

The invention discloses a novel material based on a natural diatom shell and phycobiliprotein and application. The method comprises the steps of removing organic matter contained in the diatom culturing shell; alkylating the diatom shell with APTES (3-aminopropyl trimethoxysilane), and making the alkylated diatom shell react with phycobiliprotein through BS3 (sulfosuccinyl iminooctanediate) to form covalent connection in order to achieve the purpose of fixing the phycobiliprotein to the diatom shell. The adopted diatom shell has the advantages of being convenient to obtain, relatively low in price, extremely high in biocompatibility, stability and reaction inertia and capable of achieving a certain light effect, so that a connected phycobiliprotein structure is more stable and has a sensitization effect.

Description

technical field [0001] The invention relates to the technical field of new materials, in particular to a novel material based on natural diatom shells and phycobiliproteins and its application. Background technique [0002] Phycobiliprotein is a light-harvesting pigment protein with covalently linked open-chain tetrapyrrole structure phycobilin present in cyanobacteria, red algae, cryptophyga and a few dinoflagellates; it can efficiently transfer the captured light energy to the photoreaction The center is a class of oligomer water-soluble pigment proteins, mainly including phycocyanin and phycoerythrin. Phycocyanin is the most ubiquitous phycobiliprotein and can be observed in almost all phycobiliprotein-containing organisms. Most abundant in most cyanobacterial species that grow in natural environments. These blue or blue-purple phycobiliproteins can absorb visible light from 580nm to 630nm, and emit red fluorescence intensively in the range of 635nm-645nm. Phycoerythri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K47/69A61P35/00
CPCA61K41/0057A61K47/6923A61P35/00
Inventor 赵华伟蒲洋魏梦娇李文军秦松
Owner LUDONG UNIVERSITY
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