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Method for cultivating Beauveria bassiana spore powder in a fungus bag

A technology of Beauveria bassiana and spore powder is applied in the field of microbial culture, which can solve problems such as breeding loss, bacterial contamination, bacterial silkworm disease, etc., and achieve the effects of low time cost, isolation of pollution, and easy operation.

Active Publication Date: 2021-04-09
四川省农业科学院经济作物育种栽培研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of artificial silkworm production, due to the unstable quality of Beauveria bassiana coccidioid spore powder and serious contamination by miscellaneous bacteria, there are risks of bacterial silkworm disease and incomplete rigidity, resulting in breeding losses

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A method for cultivating Beauveria bassiana spore powder in bacteria bags, comprising the following steps:

[0037] 1) Liquid medium preparation and sterilization

[0038] Liquid media require carbon and nitrogen sources and a small amount of trace elements. Carbohydrates can be used as the energy source for its growth, and agricultural by-products containing starch and sugar can be used. The organic nitrogen source can be egg white, yeast extract, corn steep liquor, soybean meal, etc., which are beneficial to the growth of Beauveria bassiana. In this embodiment, SDY medium is selected, and the ingredients are: 40 g of white sugar, 10 g of yeast extract powder, 10 g of peptone, and 1000 mL of water.

[0039] Weigh all kinds of raw materials according to the formula, put them into the pot together, boil for 5 minutes, make up to 1000mL with boiling water, stir evenly, and then put it into a glass triangle bottle with a liquid capacity of 200-250mL, and use a sterile air...

Embodiment 2

[0049] A method for cultivating Beauveria bassiana spore powder in bacteria bags, comprising the following steps:

[0050] 1) Liquid medium preparation and sterilization

[0051] Liquid media require carbon and nitrogen sources and a small amount of trace elements. Carbohydrates are used as the energy source for its growth, and agricultural by-products containing starch and sugars can be used. Protein aging, yeast extract, corn steep liquor, soybean meal, etc. are used as organic nitrogen sources, which are beneficial to the growth of Beauveria bassiana. In this example, CMA medium is selected, and the ingredients are: 20g of corn flour, 10g of wheat bran, 5g of peptone, KH 2 PO 4 3g, NH 4 NO 3 1g, MgSO 4 .7H 2 O 1g, water 1000mL.

[0052] Weigh all kinds of raw materials according to the formula, put them into the pot together, boil for 5 minutes, make up to 1000mL with boiling water, stir evenly, and then put it into a glass triangle bottle with a liquid capacity of ...

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PUM

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Abstract

The invention discloses a method for cultivating spore powder of Beauveria bassiana coccidioides in fungus bags. First, in the liquid culture stage, prepare nutrients such as carbon source and nitrogen source for the growth of Beauveria bassiana until the concentration of spores reaches 10 8 ~10 9 cells / mL transferred to solid medium. In the solid culture stage, rice is used as the culture medium. After liquid bacteria are inoculated, edible fungus cultivation bags are combined with fruiting rings and sterile air-permeable sealing films with rubber bands to form a cylindrical miniature "fermentation tank". The bacteria bag culture is used to isolate environmental microbial pollution, and it can be well ventilated, and the inner wall of the bacteria bag is used to absorb the water metabolized by Beauveria bassiana, so as to promote the rapid spore production of Beauveria bassiana. The sporulation cycle of the method is 8 days to 10 days, no need for large fermentation tank equipment, simple operation, low cost of raw materials, equipment, labor and time, and is suitable for workshop production of agricultural cooperatives. The prepared spore powder can be used for the inoculation of silkworm, a traditional Chinese medicinal material, and can also be used as a raw material to be processed into a microbial insecticide for biological control of agricultural pests.

Description

technical field [0001] The invention belongs to the technical field of microorganism cultivation, and in particular relates to a method for cultivating Beauveria bassiana spore powder in a fungus bag. Background technique [0002] Beauveria bassiana (Beauveria bassiana) belongs to Ascomycota, Hypocreaes, Cordycepsaceae, and Beauveria. It is a broad-spectrum entomopathogenic fungus, mainly asexual reproduction, producing conidia; sexual It reproduces and produces fruiting bodies, which are identified as Cordyceps coccidioides. Beauveria bassiana has been used for biological pest control worldwide. In my country, the use of Beauveria bassiana to control pine caterpillars and corn borers has achieved good pest control effects. Beauveria bassiana can also infect economic insects such as silkworms, and the dried corpses of infected silkworm larvae can be used as Chinese medicinal materials, called dead silkworms, which can dispel wind and calm convulsions when used as medicine. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N3/00A01P7/04C12R1/645
CPCC12N1/14C12N3/00
Inventor 孙佩童文龙燕梅
Owner 四川省农业科学院经济作物育种栽培研究所