Preparation method of chemical luminescence kit for placenta growth factors

Abstract

The invention discloses a preparation method of a chemical luminescence kit for placenta growth factors. The preparation method comprises the following steps of preparing streptavidin magnetic particles, and preparing a streptavidin magnetic particle suspension liquid, preparing a placenta growth factor marked by biotin, preparing a placenta growth factor antibody marked by a chemical luminescencesubstance and the like; the streptavidin magnetic particles have high affinity between streptavidin and biotin, so that any biotin-labeled molecules can be bound, and the streptavidin magnetic particles can be used for immunization and molecular detection; the preparation method adopts the polystyrene magnetic particles, and has the characteristics of uniform particle size, large specific surfacearea and regular morphology, so that the target molecule can be rapidly and efficiently captured and magnetic separation is realized; and the magnetic particle chemiluminescence method provided by the invention has strong specificity, accuracy and quickness, short in detection time, accurate in detection result, high in repeatability and the like.

Description

technical field [0001] The invention relates to the field of in vitro diagnosis, in particular to a preparation method of a chemiluminescent kit of placental growth factor. Background technique [0002] Placental growth factor (PLGF) was first isolated and purified from human placental cDNA library by Maglione et al. in 1991. PLGF is mainly synthesized by syncytiotrophoblast cells and can bind to tyrosinase receptors located in trophoblast cells and vascular endothelial cells. It is a protein that has autocrine effects on trophoblast cell functions and paracrine effects on angiogenesis. PLGF has a unique regulatory effect on the function of trophoblast cells and endothelial cells, and can promote angiogenesis. Detecting the blood PLGF level of pregnant women can be used clinically to identify the oxygen supply pressure of placental syncytiotrophoblast cells, and to predict, identify and monitor treatment of preeclampsia. [0003] So far, the methods used to detect placenta...

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Problems solved by technology

[0003] So far, the methods used to detect placental growth factor residues in human serum mainly include: enzyme-linked immunoassay (ELISA). However, although the detection price of ELISA is low and fast, the sensitivity is not enough, and it is only suitable for the detection of trace substances. Detection and identification; the method adopted in the present invention is the direct chemiluminescence method, and the use of acridinium ester as a chemiluminescent marker has obvious advantages, mainly in that the reaction does not require a catalyst, as long as the alkaline environment can be carried out, the reaction is rapid, and can be completely captured Photons generated by reaction, low background luminescence, high signal-to-noise ratio, less interference factors, good reagent stability, simple system, low cost of excitation solution, acridinium ester is easy to bind with protein, and the photon yield does not decrease after the association