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A preparation method and application method of a phosphorylated protein enrichment material

A phosphorylated protein, application method technology, applied in the preparation method of peptide, chemical instrument and method, separation method and other directions, can solve the problems of weak interaction, easy loss of metal ions, etc., achieve simple synthesis process, and achieve selective separation , to achieve the effect of enrichment and purification

Active Publication Date: 2021-05-14
NORTHEASTERN UNIV LIAONING
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  • Description
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Problems solved by technology

However, the traditional metal chelating reagents, iminodiacetic acid and nitrilotriacetic acid, have weak interactions with solid-phase supports, and metal ions are easily lost. Therefore, a simple chelating reagent was chosen to develop a new The adsorption material is very important for the enrichment of phosphorylated proteins

Method used

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  • A preparation method and application method of a phosphorylated protein enrichment material
  • A preparation method and application method of a phosphorylated protein enrichment material
  • A preparation method and application method of a phosphorylated protein enrichment material

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Embodiment 1

[0069] Embodiment 1: the synthesis of titanium chelated polydopamine-polyoxometalate composite material

[0070] (1) Dissolve 33g of sodium tungstate dihydrate in 30mL of water, and add 26.5mL of 85% phosphoric acid. After magnetic stirring for 30 min, the mixed solution was transferred to a 150 mL reactor, and subjected to hydrothermal reaction at 120 °C for 12 h. After the reaction kettle was cooled to room temperature, there were light green crystals on the polytetrafluoroethylene wall of the liner, the product was taken out, 15mL of water was added, and then 10g of potassium chloride was added to form a white precipitate. After stirring for 1 h, until the mass of the white precipitate no longer changed, the precipitate was collected by suction filtration, washed with methanol three times, and dried at 60°C. Finally, dissolve the solid in 30mL of water at 70°C, the solid dissolves, and white crystals precipitate out after cooling, filter under reduced pressure, and dry at ...

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Abstract

A preparation method and application method of a phosphorylated protein enrichment material of the present invention. During the preparation, after preparing the corresponding polyoxometalate, the composite microsphere formed by the polymerization of dopamine and polyoxometalate is used as a carrier, and then metal ions are fixed on the surface of the microsphere to obtain the enriched material product. The obtained final product is used as an adsorption material for the selective separation and enrichment of phosphorylated proteins. The applied dopamine has good water solubility, non-toxic and harmless and strong metal bonding ability. In addition, both the polyoxometalate and the phosphate group have π bonds, which can cause π stacking to further promote the adsorption of phosphorylated proteins. The material has the advantages of simple synthesis process, low cost, high selectivity and the like.

Description

technical field [0001] The invention belongs to the technical field of protein separation and enrichment, and in particular relates to the preparation and application method of a phosphorylated protein enrichment material. Background technique [0002] Phosphorylated protein, as a very important post-translational modification protein, plays an important role in many biological processes, such as signal transduction, molecular recognition, cell differentiation and metabolism. Abnormal protein phosphorylation levels are involved in the pathogenesis of many human diseases, such as cancer, heart disease, and cardiovascular disease. However, the low content of phosphorylated proteins in biological samples limits their study. Therefore, in order to study the phosphorylation modification of proteins in depth, it is necessary to use enrichment and purification methods to separate low-abundance phosphorylated proteins from complex real samples. [0003] Immobilized metal ion affin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/281B01J20/30B01D15/20C07K1/22
CPCB01D15/20B01J20/281C07K1/22
Inventor 陈帅王蒙蒙王建华于永亮
Owner NORTHEASTERN UNIV LIAONING
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